Snake deltavirus F18-5
Average proteome isoelectric point is 8.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G1TVA1|A0A3G1TVA1_9VIRU Delta antigen OS=Snake deltavirus F18-5 OX=2419666 PE=3 SV=1
MM1 pKa = 7.07 EE2 pKa = 4.94 TPSKK6 pKa = 10.54 KK7 pKa = 10.14 QIPTPSRR14 pKa = 11.84 EE15 pKa = 4.52 DD16 pKa = 3.29 ILEE19 pKa = 3.91 QWVEE23 pKa = 3.47 LGKK26 pKa = 10.53 RR27 pKa = 11.84 KK28 pKa = 9.99 KK29 pKa = 9.97 EE30 pKa = 4.08 LEE32 pKa = 4.09 KK33 pKa = 10.69 EE34 pKa = 4.19 LQKK37 pKa = 9.68 VTKK40 pKa = 9.91 KK41 pKa = 10.19 KK42 pKa = 10.44 RR43 pKa = 11.84 KK44 pKa = 9.79 LEE46 pKa = 4.11 EE47 pKa = 3.55 QHH49 pKa = 6.62 GFLGNVLGIVRR60 pKa = 11.84 GKK62 pKa = 7.21 EE63 pKa = 3.75 QKK65 pKa = 9.97 PAATPQKK72 pKa = 9.95 KK73 pKa = 9.59 RR74 pKa = 11.84 KK75 pKa = 9.47 AEE77 pKa = 3.74 EE78 pKa = 4.07 SMDD81 pKa = 3.41 VDD83 pKa = 3.76 GGSRR87 pKa = 11.84 LPPKK91 pKa = 9.7 EE92 pKa = 3.42 IKK94 pKa = 10.15 KK95 pKa = 10.5 RR96 pKa = 11.84 IFTEE100 pKa = 3.99 EE101 pKa = 3.15 EE102 pKa = 3.72 RR103 pKa = 11.84 AEE105 pKa = 3.98 HH106 pKa = 6.53 RR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 GQLEE113 pKa = 4.04 NKK115 pKa = 9.36 KK116 pKa = 10.12 KK117 pKa = 9.88 QLEE120 pKa = 4.33 GRR122 pKa = 11.84 GKK124 pKa = 9.61 QLPEE128 pKa = 4.05 EE129 pKa = 4.2 EE130 pKa = 4.17 QKK132 pKa = 11.29 LLAEE136 pKa = 4.7 LTRR139 pKa = 11.84 KK140 pKa = 10.4 DD141 pKa = 3.54 EE142 pKa = 3.94 EE143 pKa = 4.08 RR144 pKa = 11.84 KK145 pKa = 9.71 QRR147 pKa = 11.84 FHH149 pKa = 7.58 YY150 pKa = 10.47 GGAGEE155 pKa = 4.2 VNPLEE160 pKa = 4.53 GQSRR164 pKa = 11.84 GAFGGGFVPSTQGVPEE180 pKa = 4.45 SPFHH184 pKa = 5.77 RR185 pKa = 11.84 TGTGLDD191 pKa = 3.36 VRR193 pKa = 11.84 GDD195 pKa = 3.28 KK196 pKa = 9.93 MFPP199 pKa = 3.24
Molecular weight: 22.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.849
IPC2_protein 8.946
IPC_protein 8.858
Toseland 10.145
ProMoST 9.545
Dawson 10.204
Bjellqvist 9.736
Wikipedia 10.277
Rodwell 10.906
Grimsley 10.218
Solomon 10.233
Lehninger 10.233
Nozaki 10.101
DTASelect 9.736
Thurlkill 10.101
EMBOSS 10.511
Sillero 10.116
Patrickios 10.643
IPC_peptide 10.248
IPC2_peptide 7.966
IPC2.peptide.svr19 7.994
Protein with the highest isoelectric point:
>tr|A0A3G1TVA1|A0A3G1TVA1_9VIRU Delta antigen OS=Snake deltavirus F18-5 OX=2419666 PE=3 SV=1
MM1 pKa = 7.07 EE2 pKa = 4.94 TPSKK6 pKa = 10.54 KK7 pKa = 10.14 QIPTPSRR14 pKa = 11.84 EE15 pKa = 4.52 DD16 pKa = 3.29 ILEE19 pKa = 3.91 QWVEE23 pKa = 3.47 LGKK26 pKa = 10.53 RR27 pKa = 11.84 KK28 pKa = 9.99 KK29 pKa = 9.97 EE30 pKa = 4.08 LEE32 pKa = 4.09 KK33 pKa = 10.69 EE34 pKa = 4.19 LQKK37 pKa = 9.68 VTKK40 pKa = 9.91 KK41 pKa = 10.19 KK42 pKa = 10.44 RR43 pKa = 11.84 KK44 pKa = 9.79 LEE46 pKa = 4.11 EE47 pKa = 3.55 QHH49 pKa = 6.62 GFLGNVLGIVRR60 pKa = 11.84 GKK62 pKa = 7.21 EE63 pKa = 3.75 QKK65 pKa = 9.97 PAATPQKK72 pKa = 9.95 KK73 pKa = 9.59 RR74 pKa = 11.84 KK75 pKa = 9.47 AEE77 pKa = 3.74 EE78 pKa = 4.07 SMDD81 pKa = 3.41 VDD83 pKa = 3.76 GGSRR87 pKa = 11.84 LPPKK91 pKa = 9.7 EE92 pKa = 3.42 IKK94 pKa = 10.15 KK95 pKa = 10.5 RR96 pKa = 11.84 IFTEE100 pKa = 3.99 EE101 pKa = 3.15 EE102 pKa = 3.72 RR103 pKa = 11.84 AEE105 pKa = 3.98 HH106 pKa = 6.53 RR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 GQLEE113 pKa = 4.04 NKK115 pKa = 9.36 KK116 pKa = 10.12 KK117 pKa = 9.88 QLEE120 pKa = 4.33 GRR122 pKa = 11.84 GKK124 pKa = 9.61 QLPEE128 pKa = 4.05 EE129 pKa = 4.2 EE130 pKa = 4.17 QKK132 pKa = 11.29 LLAEE136 pKa = 4.7 LTRR139 pKa = 11.84 KK140 pKa = 10.4 DD141 pKa = 3.54 EE142 pKa = 3.94 EE143 pKa = 4.08 RR144 pKa = 11.84 KK145 pKa = 9.71 QRR147 pKa = 11.84 FHH149 pKa = 7.58 YY150 pKa = 10.47 GGAGEE155 pKa = 4.2 VNPLEE160 pKa = 4.53 GQSRR164 pKa = 11.84 GAFGGGFVPSTQGVPEE180 pKa = 4.45 SPFHH184 pKa = 5.77 RR185 pKa = 11.84 TGTGLDD191 pKa = 3.36 VRR193 pKa = 11.84 GDD195 pKa = 3.28 KK196 pKa = 9.93 MFPP199 pKa = 3.24
Molecular weight: 22.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.849
IPC2_protein 8.946
IPC_protein 8.858
Toseland 10.145
ProMoST 9.545
Dawson 10.204
Bjellqvist 9.736
Wikipedia 10.277
Rodwell 10.906
Grimsley 10.218
Solomon 10.233
Lehninger 10.233
Nozaki 10.101
DTASelect 9.736
Thurlkill 10.101
EMBOSS 10.511
Sillero 10.116
Patrickios 10.643
IPC_peptide 10.248
IPC2_peptide 7.966
IPC2.peptide.svr19 7.994
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
199
199
199
199.0
22.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.518 ± 0.0
0.0 ± 0.0
3.015 ± 0.0
14.07 ± 0.0
3.518 ± 0.0
11.055 ± 0.0
2.01 ± 0.0
2.513 ± 0.0
13.568 ± 0.0
8.04 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.508 ± 0.0
1.508 ± 0.0
6.533 ± 0.0
6.533 ± 0.0
9.045 ± 0.0
3.518 ± 0.0
4.523 ± 0.0
4.523 ± 0.0
0.503 ± 0.0
0.503 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here