Capybara microvirus Cap1_SP_159
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W597|A0A4P8W597_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_159 OX=2585395 PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 9.65 PDD4 pKa = 4.23 SPDD7 pKa = 3.2 STHH10 pKa = 7.1 DD11 pKa = 3.03 RR12 pKa = 11.84 TYY14 pKa = 11.35 LVAVRR19 pKa = 11.84 DD20 pKa = 3.95 KK21 pKa = 10.57 STPQCIMFPRR31 pKa = 11.84 LLPMGAIRR39 pKa = 11.84 QYY41 pKa = 11.19 KK42 pKa = 9.08 HH43 pKa = 7.22 DD44 pKa = 4.05 IGNFILKK51 pKa = 10.46 SPDD54 pKa = 2.93 SAYY57 pKa = 10.74 ALYY60 pKa = 9.83 PEE62 pKa = 5.54 EE63 pKa = 3.9 YY64 pKa = 10.23 AIVCIEE70 pKa = 4.15 IDD72 pKa = 3.35 NANDD76 pKa = 3.27 FFEE79 pKa = 4.8 VCDD82 pKa = 4.52 FRR84 pKa = 11.84 DD85 pKa = 3.77 CKK87 pKa = 10.63 FLAEE91 pKa = 3.85 EE92 pKa = 4.26 AQRR95 pKa = 11.84 KK96 pKa = 5.33 TSEE99 pKa = 4.1 VKK101 pKa = 9.98 TDD103 pKa = 3.29 EE104 pKa = 4.86 DD105 pKa = 4.03 NNN107 pKa = 3.94
Molecular weight: 12.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.61
IPC2_protein 4.685
IPC_protein 4.584
Toseland 4.418
ProMoST 4.724
Dawson 4.558
Bjellqvist 4.698
Wikipedia 4.457
Rodwell 4.431
Grimsley 4.329
Solomon 4.546
Lehninger 4.507
Nozaki 4.673
DTASelect 4.876
Thurlkill 4.444
EMBOSS 4.469
Sillero 4.711
Patrickios 3.694
IPC_peptide 4.558
IPC2_peptide 4.698
IPC2.peptide.svr19 4.695
Protein with the highest isoelectric point:
>tr|A0A4P8W4F9|A0A4P8W4F9_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_159 OX=2585395 PE=4 SV=1
MM1 pKa = 7.4 SWLKK5 pKa = 10.77 KK6 pKa = 10.29 AFKK9 pKa = 10.12 KK10 pKa = 9.91 IKK12 pKa = 10.06 KK13 pKa = 8.8 AVKK16 pKa = 10.32 NPTHH20 pKa = 6.62 SKK22 pKa = 10.73 AFRR25 pKa = 11.84 HH26 pKa = 5.37 VLNATSGGLAGEE38 pKa = 4.48 IAHH41 pKa = 6.76 HH42 pKa = 6.15 LNKK45 pKa = 9.96 ARR47 pKa = 11.84 RR48 pKa = 11.84 KK49 pKa = 9.34 VGLSGKK55 pKa = 8.82 SIKK58 pKa = 10.27 SALSSRR64 pKa = 11.84 ATGASNTALTLSGSSMSRR82 pKa = 11.84 QPMGNTGFHH91 pKa = 7.02 RR92 pKa = 11.84 YY93 pKa = 9.24 GMM95 pKa = 4.22
Molecular weight: 10.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.209
IPC2_protein 10.643
IPC_protein 11.871
Toseland 12.076
ProMoST 12.501
Dawson 12.091
Bjellqvist 12.032
Wikipedia 12.515
Rodwell 12.193
Grimsley 12.12
Solomon 12.53
Lehninger 12.442
Nozaki 12.076
DTASelect 12.032
Thurlkill 12.076
EMBOSS 12.559
Sillero 12.076
Patrickios 11.93
IPC_peptide 12.544
IPC2_peptide 11.491
IPC2.peptide.svr19 8.864
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1504
95
556
250.7
28.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.713 ± 0.888
1.862 ± 0.632
6.25 ± 0.767
5.585 ± 0.703
4.787 ± 0.773
6.25 ± 0.673
2.992 ± 0.335
5.519 ± 0.431
5.718 ± 0.947
8.71 ± 0.724
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.928 ± 0.373
5.319 ± 1.128
5.253 ± 0.713
3.457 ± 0.4
5.452 ± 0.211
8.112 ± 0.57
5.12 ± 0.225
4.987 ± 0.543
1.197 ± 0.262
3.79 ± 0.389
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here