Streptococcus virus 858
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B0YL82|B0YL82_9CAUD Orf32 OS=Streptococcus virus 858 OX=459845 PE=4 SV=1
MM1 pKa = 7.13 VNWVDD6 pKa = 4.56 ANGNDD11 pKa = 4.18 LPDD14 pKa = 4.84 GADD17 pKa = 3.18 QDD19 pKa = 4.92 FKK21 pKa = 11.67 AGMYY25 pKa = 10.17 FSFSGDD31 pKa = 3.22 EE32 pKa = 4.18 TNITDD37 pKa = 3.65 TGEE40 pKa = 3.72 GGYY43 pKa = 10.61 YY44 pKa = 9.76 GGYY47 pKa = 8.0 YY48 pKa = 9.41 YY49 pKa = 10.76 RR50 pKa = 11.84 RR51 pKa = 11.84 FEE53 pKa = 4.17 FGQFGPVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.857
IPC2_protein 3.77
IPC_protein 3.706
Toseland 3.49
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.719
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.834
Patrickios 0.095
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.757
Protein with the highest isoelectric point:
>tr|B0YL52|B0YL52_9CAUD Orf2 OS=Streptococcus virus 858 OX=459845 PE=4 SV=1
MM1 pKa = 6.88 FVSWVKK7 pKa = 9.47 TKK9 pKa = 10.51 KK10 pKa = 9.95 DD11 pKa = 3.14 RR12 pKa = 11.84 HH13 pKa = 5.0 NCRR16 pKa = 11.84 HH17 pKa = 4.94 FMKK20 pKa = 10.63 VNTTIISEE28 pKa = 4.24 RR29 pKa = 11.84 LVQCYY34 pKa = 8.99 CRR36 pKa = 11.84 KK37 pKa = 10.06 LMRR40 pKa = 11.84 KK41 pKa = 7.06 QHH43 pKa = 6.18 SNVANASFEE52 pKa = 4.43 NIHH55 pKa = 6.89 AGEE58 pKa = 4.18 RR59 pKa = 11.84 LHH61 pKa = 7.1 TIALNRR67 pKa = 11.84 KK68 pKa = 8.14
Molecular weight: 8.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.575
IPC_protein 9.838
Toseland 10.76
ProMoST 10.365
Dawson 10.818
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 11.257
Grimsley 10.847
Solomon 10.906
Lehninger 10.891
Nozaki 10.76
DTASelect 10.438
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.745
Patrickios 11.023
IPC_peptide 10.921
IPC2_peptide 9.502
IPC2.peptide.svr19 8.588
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
11098
40
1617
241.3
26.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.794 ± 1.213
0.595 ± 0.144
5.893 ± 0.454
6.398 ± 0.739
4.01 ± 0.18
7.109 ± 0.6
1.252 ± 0.176
6.713 ± 0.407
7.668 ± 0.678
7.38 ± 0.4
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.433 ± 0.292
5.668 ± 0.376
2.82 ± 0.277
3.766 ± 0.255
4.316 ± 0.421
7.272 ± 0.597
6.902 ± 0.46
6.695 ± 0.298
1.289 ± 0.21
4.028 ± 0.456
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here