Duck circovirus
Average proteome isoelectric point is 7.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I2CZK4|I2CZK4_9CIRC Capsid protein OS=Duck circovirus OX=324685 GN=Cap PE=3 SV=1
MM1 pKa = 7.62 AKK3 pKa = 10.03 SGNYY7 pKa = 9.34 SYY9 pKa = 11.03 KK10 pKa = 10.22 RR11 pKa = 11.84 WVFTINNPTFEE22 pKa = 5.38 DD23 pKa = 4.37 YY24 pKa = 11.38 VHH26 pKa = 6.99 VLEE29 pKa = 6.12 FCTLDD34 pKa = 3.2 NCKK37 pKa = 9.85 FAIVGEE43 pKa = 4.22 EE44 pKa = 3.75 KK45 pKa = 10.66 GANGTPHH52 pKa = 6.68 LQGFLNLRR60 pKa = 11.84 SNARR64 pKa = 11.84 AAALEE69 pKa = 3.96 EE70 pKa = 4.42 SLGGRR75 pKa = 11.84 AWLSRR80 pKa = 11.84 ARR82 pKa = 11.84 GSDD85 pKa = 3.31 EE86 pKa = 5.71 DD87 pKa = 4.0 NEE89 pKa = 4.41 EE90 pKa = 4.14 YY91 pKa = 10.26 CAKK94 pKa = 10.09 EE95 pKa = 3.72 STYY98 pKa = 10.94 LRR100 pKa = 11.84 VGEE103 pKa = 4.2 PVSKK107 pKa = 10.61 GRR109 pKa = 11.84 SSDD112 pKa = 3.5 LAEE115 pKa = 4.15 ATSAVMAGVPLTEE128 pKa = 4.41 VARR131 pKa = 11.84 KK132 pKa = 9.21 FPTTYY137 pKa = 10.62 VIFGRR142 pKa = 11.84 GLEE145 pKa = 4.13 RR146 pKa = 11.84 LRR148 pKa = 11.84 HH149 pKa = 6.05 LIVEE153 pKa = 4.56 TQRR156 pKa = 11.84 DD157 pKa = 3.66 WKK159 pKa = 9.91 TEE161 pKa = 4.0 VIVLIGPPGTGKK173 pKa = 10.04 SRR175 pKa = 11.84 YY176 pKa = 9.4 AFEE179 pKa = 4.95 FPAEE183 pKa = 3.93 NKK185 pKa = 9.86 YY186 pKa = 10.59 YY187 pKa = 10.65 KK188 pKa = 10.16 PRR190 pKa = 11.84 GKK192 pKa = 8.91 WWDD195 pKa = 3.9 GYY197 pKa = 10.85 SGNDD201 pKa = 3.3 VVVMDD206 pKa = 5.83 DD207 pKa = 3.98 FYY209 pKa = 11.92 GWLPYY214 pKa = 10.72 DD215 pKa = 4.0 DD216 pKa = 5.54 LLRR219 pKa = 11.84 ITDD222 pKa = 4.33 RR223 pKa = 11.84 YY224 pKa = 8.36 PLRR227 pKa = 11.84 VEE229 pKa = 4.85 FKK231 pKa = 10.75 GGMTQFVAKK240 pKa = 9.46 TLIITSNRR248 pKa = 11.84 EE249 pKa = 3.17 PRR251 pKa = 11.84 DD252 pKa = 3.33 WYY254 pKa = 10.83 KK255 pKa = 11.13 SEE257 pKa = 4.41 FDD259 pKa = 3.61 LSALYY264 pKa = 10.34 RR265 pKa = 11.84 RR266 pKa = 11.84 INKK269 pKa = 9.29 YY270 pKa = 10.12 LVYY273 pKa = 10.73 NIDD276 pKa = 3.46 KK277 pKa = 10.73 YY278 pKa = 11.39 EE279 pKa = 4.14 PAQACTLPFPINYY292 pKa = 9.74
Molecular weight: 33.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.674
IPC2_protein 6.868
IPC_protein 7.073
Toseland 6.781
ProMoST 7.688
Dawson 7.673
Bjellqvist 7.6
Wikipedia 7.6
Rodwell 7.644
Grimsley 6.825
Solomon 7.775
Lehninger 7.79
Nozaki 7.805
DTASelect 7.761
Thurlkill 7.79
EMBOSS 7.907
Sillero 8.039
Patrickios 4.088
IPC_peptide 7.775
IPC2_peptide 7.088
IPC2.peptide.svr19 7.327
Protein with the highest isoelectric point:
>tr|I2CZK4|I2CZK4_9CIRC Capsid protein OS=Duck circovirus OX=324685 GN=Cap PE=3 SV=1
MM1 pKa = 8.09 RR2 pKa = 11.84 GRR4 pKa = 11.84 TYY6 pKa = 10.46 RR7 pKa = 11.84 RR8 pKa = 11.84 AYY10 pKa = 9.23 RR11 pKa = 11.84 GRR13 pKa = 11.84 RR14 pKa = 11.84 KK15 pKa = 9.89 RR16 pKa = 11.84 RR17 pKa = 11.84 GLRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 FRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 LRR29 pKa = 11.84 IARR32 pKa = 11.84 PRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 FSVVTYY42 pKa = 10.07 KK43 pKa = 9.3 VTRR46 pKa = 11.84 NTVFGFFGSQTGPTAAGKK64 pKa = 8.0 WQSLSLEE71 pKa = 4.75 DD72 pKa = 3.59 GAQYY76 pKa = 10.5 TDD78 pKa = 3.7 PPARR82 pKa = 11.84 GNNICGLNMRR92 pKa = 11.84 WAMFGDD98 pKa = 3.86 TNSYY102 pKa = 7.24 MTGSTPFYY110 pKa = 9.79 HH111 pKa = 6.53 YY112 pKa = 10.3 PYY114 pKa = 9.79 DD115 pKa = 3.64 YY116 pKa = 11.93 YY117 pKa = 10.47 MIKK120 pKa = 10.47 GVAITLRR127 pKa = 11.84 PAYY130 pKa = 10.29 NIYY133 pKa = 10.31 QKK135 pKa = 11.11 SKK137 pKa = 7.74 TQGSTVIDD145 pKa = 3.45 KK146 pKa = 10.75 DD147 pKa = 3.68 GQIVKK152 pKa = 9.55 TSTTGWSIDD161 pKa = 3.79 PYY163 pKa = 10.79 GSTSSRR169 pKa = 11.84 RR170 pKa = 11.84 TWDD173 pKa = 3.07 PSRR176 pKa = 11.84 VHH178 pKa = 6.3 RR179 pKa = 11.84 RR180 pKa = 11.84 YY181 pKa = 10.13 FIPKK185 pKa = 9.62 PIIQRR190 pKa = 11.84 AGEE193 pKa = 4.15 GTKK196 pKa = 10.74 YY197 pKa = 9.78 STFFLGGRR205 pKa = 11.84 NFTWINCAQDD215 pKa = 3.56 QVVHH219 pKa = 6.74 YY220 pKa = 10.14 GMGMSLRR227 pKa = 11.84 KK228 pKa = 9.19 PDD230 pKa = 3.16 NTTGVNAQYY239 pKa = 10.62 DD240 pKa = 3.7 IEE242 pKa = 4.33 AQFTFYY248 pKa = 10.69 IKK250 pKa = 10.53 FGQFTGFF257 pKa = 3.35
Molecular weight: 29.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 10.218
IPC_protein 11.082
Toseland 10.862
ProMoST 10.833
Dawson 10.994
Bjellqvist 10.818
Wikipedia 11.301
Rodwell 11.052
Grimsley 11.067
Solomon 11.184
Lehninger 11.125
Nozaki 10.847
DTASelect 10.818
Thurlkill 10.891
EMBOSS 11.301
Sillero 10.935
Patrickios 10.701
IPC_peptide 11.184
IPC2_peptide 9.984
IPC2.peptide.svr19 7.972
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
549
257
292
274.5
31.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.193 ± 0.762
1.093 ± 0.211
4.918 ± 0.428
4.554 ± 2.275
5.464 ± 0.511
8.743 ± 0.923
1.093 ± 0.05
4.736 ± 0.478
5.282 ± 0.412
5.829 ± 1.824
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.004 ± 0.484
4.372 ± 0.323
4.918 ± 0.167
2.914 ± 1.179
10.018 ± 1.896
5.829 ± 0.267
7.65 ± 1.395
5.464 ± 0.795
2.186 ± 0.161
6.74 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here