Carrot red leaf virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 7.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q670I1|Q670I1_9LUTE Coat protein OS=Carrot red leaf virus OX=66200 GN=p3 PE=4 SV=1
MM1 pKa = 8.2DD2 pKa = 4.61SPRR5 pKa = 11.84EE6 pKa = 3.94QQEE9 pKa = 4.15LNPWLLSTHH18 pKa = 6.04LQGAHH23 pKa = 5.55VDD25 pKa = 3.61VHH27 pKa = 6.5GLSQSLWYY35 pKa = 9.91KK36 pKa = 9.9PHH38 pKa = 6.92EE39 pKa = 4.38LDD41 pKa = 5.25EE42 pKa = 6.43DD43 pKa = 4.35DD44 pKa = 5.78EE45 pKa = 4.71DD46 pKa = 6.2AEE48 pKa = 4.36DD49 pKa = 3.73AALVTHH55 pKa = 6.38EE56 pKa = 4.03QWEE59 pKa = 4.39DD60 pKa = 3.49PEE62 pKa = 5.51AEE64 pKa = 4.13SVARR68 pKa = 11.84SLCFRR73 pKa = 11.84RR74 pKa = 11.84TTSRR78 pKa = 11.84AAPVEE83 pKa = 4.27SSPSARR89 pKa = 11.84IYY91 pKa = 9.51QTTLLSAMDD100 pKa = 3.87YY101 pKa = 10.68SRR103 pKa = 11.84HH104 pKa = 4.48STNIKK109 pKa = 10.12SQTCEE114 pKa = 3.74YY115 pKa = 8.26VTSRR119 pKa = 11.84KK120 pKa = 8.6PHH122 pKa = 5.31QPPVARR128 pKa = 11.84SLMSWTPVVNSINFRR143 pKa = 11.84AQSTNSGSSRR153 pKa = 11.84MVKK156 pKa = 10.14PLGALKK162 pKa = 10.55RR163 pKa = 11.84LMGRR167 pKa = 11.84SGTPLRR173 pKa = 11.84RR174 pKa = 11.84TNSGSSGRR182 pKa = 11.84EE183 pKa = 3.34MEE185 pKa = 4.48VIRR188 pKa = 11.84RR189 pKa = 3.84

Molecular weight: 21.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q670I2|Q670I2_9LUTE Readthrough protein OS=Carrot red leaf virus OX=66200 GN=p5 PE=3 SV=1
MM1 pKa = 7.21ATYY4 pKa = 10.96GLTKK8 pKa = 10.09RR9 pKa = 11.84ATGIKK14 pKa = 9.88PMVVVNTPSRR24 pKa = 11.84RR25 pKa = 11.84PRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84TRR31 pKa = 11.84TKK33 pKa = 10.12PIVVVQTARR42 pKa = 11.84TGRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84GRR53 pKa = 11.84GSGNPRR59 pKa = 11.84TMGGSGGGISSQKK72 pKa = 10.3FVFSKK77 pKa = 11.19DD78 pKa = 3.4NIAGSSGGVLTFGKK92 pKa = 10.25DD93 pKa = 3.58LSDD96 pKa = 4.34HH97 pKa = 6.6PAFSNGLLKK106 pKa = 10.69AFHH109 pKa = 6.29QYY111 pKa = 10.85KK112 pKa = 7.29ITNMRR117 pKa = 11.84VRR119 pKa = 11.84YY120 pKa = 9.57ISEE123 pKa = 4.23APSTAGGSISYY134 pKa = 9.94EE135 pKa = 3.8LDD137 pKa = 3.45PSCEE141 pKa = 3.99LDD143 pKa = 3.55KK144 pKa = 11.43LSSTIYY150 pKa = 10.67KK151 pKa = 10.45FGVLKK156 pKa = 10.76NGQASWSAEE165 pKa = 3.91KK166 pKa = 10.66INGKK170 pKa = 7.67EE171 pKa = 3.68WHH173 pKa = 6.89SSSEE177 pKa = 3.86NQFRR181 pKa = 11.84FLWKK185 pKa = 10.69GNGSNSTVGSFEE197 pKa = 3.46IHH199 pKa = 4.83YY200 pKa = 8.62TCLFQNPKK208 pKa = 10.32

Molecular weight: 22.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3011

189

1045

501.8

55.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.978 ± 0.596

1.661 ± 0.376

4.351 ± 0.743

5.679 ± 0.472

4.052 ± 0.303

7.572 ± 0.68

2.325 ± 0.284

4.517 ± 0.296

5.812 ± 0.73

9.0 ± 0.964

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.86 ± 0.212

4.915 ± 0.235

5.878 ± 0.652

3.753 ± 0.132

5.978 ± 0.808

9.698 ± 0.645

6.211 ± 0.434

5.878 ± 0.132

1.727 ± 0.212

3.122 ± 0.476
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski