Escherichia phage YDC107_1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ravinvirus; unclassified Ravinvirus

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R9YJJ5|A0A2R9YJJ5_9CAUD Tail attachment protein OS=Escherichia phage YDC107_1 OX=2056767 GN=FII PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 9.4HH3 pKa = 5.21TEE5 pKa = 3.58LRR7 pKa = 11.84AAVLDD12 pKa = 3.88ALEE15 pKa = 4.33KK16 pKa = 10.45HH17 pKa = 6.2DD18 pKa = 4.25TGATLFDD25 pKa = 3.55GRR27 pKa = 11.84PAVFDD32 pKa = 4.27EE33 pKa = 4.49EE34 pKa = 4.78DD35 pKa = 3.86FPAIAVYY42 pKa = 8.59LTGAEE47 pKa = 4.22YY48 pKa = 9.85TGEE51 pKa = 4.09EE52 pKa = 4.61LDD54 pKa = 3.6SDD56 pKa = 3.79TWQAEE61 pKa = 3.69LHH63 pKa = 6.35IEE65 pKa = 4.19VFLPAQVPDD74 pKa = 4.05SEE76 pKa = 4.67LDD78 pKa = 2.98SWMEE82 pKa = 3.72SRR84 pKa = 11.84IYY86 pKa = 10.48PVMSDD91 pKa = 3.25IPALSDD97 pKa = 5.02LITSMVASGYY107 pKa = 10.36DD108 pKa = 3.32YY109 pKa = 11.21RR110 pKa = 11.84RR111 pKa = 11.84DD112 pKa = 3.5DD113 pKa = 5.7DD114 pKa = 4.64AGLWSSADD122 pKa = 3.34LTYY125 pKa = 11.13VITYY129 pKa = 9.4EE130 pKa = 3.98MM131 pKa = 4.92

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6TC89|A0A2I6TC89_9CAUD Portal protein OS=Escherichia phage YDC107_1 OX=2056767 GN=YDC107_5375 PE=3 SV=1
MM1 pKa = 7.53LFAIPRR7 pKa = 11.84SQRR10 pKa = 11.84QQRR13 pKa = 11.84WNITDD18 pKa = 2.93GRR20 pKa = 11.84LDD22 pKa = 3.53VGFHH26 pKa = 5.81KK27 pKa = 11.01VFGIAIRR34 pKa = 11.84RR35 pKa = 11.84QPDD38 pKa = 2.95KK39 pKa = 11.17CVALLMLYY47 pKa = 10.05EE48 pKa = 4.54IGINTQQHH56 pKa = 5.87FGITNTGRR64 pKa = 11.84LHH66 pKa = 6.9HH67 pKa = 6.58IHH69 pKa = 6.51LADD72 pKa = 3.23VVAAHH77 pKa = 7.64RR78 pKa = 11.84IHH80 pKa = 7.58DD81 pKa = 4.4GPLKK85 pKa = 9.9GQCFPAPFLVCGFFRR100 pKa = 11.84EE101 pKa = 4.09IVIGIRR107 pKa = 11.84PFNGGLWLRR116 pKa = 11.84PEE118 pKa = 4.11QGTKK122 pKa = 10.19PGADD126 pKa = 3.25KK127 pKa = 10.64MPQPVNLTVRR137 pKa = 11.84YY138 pKa = 8.47IHH140 pKa = 6.5SATPVSVHH148 pKa = 6.14PARR151 pKa = 11.84LLCSWLL157 pKa = 3.58

Molecular weight: 17.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

13102

60

1327

218.4

24.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.228 ± 0.55

1.229 ± 0.151

5.984 ± 0.219

6.663 ± 0.312

3.793 ± 0.223

6.61 ± 0.358

1.763 ± 0.134

5.098 ± 0.245

5.617 ± 0.3

8.487 ± 0.224

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.007 ± 0.155

3.809 ± 0.206

3.908 ± 0.211

4.205 ± 0.267

7.243 ± 0.276

6.564 ± 0.271

5.709 ± 0.291

6.549 ± 0.26

1.572 ± 0.142

2.961 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski