Streptococcus phage Javan100

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ASU0|A0A4D6ASU0_9CAUD PBECR4 domain-containing protein OS=Streptococcus phage Javan100 OX=2547973 GN=Javan100_0011 PE=4 SV=1
MM1 pKa = 7.65ALFGVDD7 pKa = 2.95ISEE10 pKa = 4.39HH11 pKa = 5.92NGFIDD16 pKa = 5.2FDD18 pKa = 3.8QLKK21 pKa = 10.24NNVDD25 pKa = 3.94FVIIRR30 pKa = 11.84SSWGSFAEE38 pKa = 4.11DD39 pKa = 2.86LRR41 pKa = 11.84ARR43 pKa = 11.84RR44 pKa = 11.84NASEE48 pKa = 4.39CEE50 pKa = 4.02RR51 pKa = 11.84VGIPYY56 pKa = 10.47GFYY59 pKa = 10.13HH60 pKa = 7.08YY61 pKa = 10.86SYY63 pKa = 11.36ACNLGDD69 pKa = 4.38AQAEE73 pKa = 4.31VNAFLNFTRR82 pKa = 11.84QFHH85 pKa = 6.86PSMPLYY91 pKa = 10.19IDD93 pKa = 4.09MEE95 pKa = 4.63DD96 pKa = 3.69ADD98 pKa = 3.86GWKK101 pKa = 10.51ANNGGVSWEE110 pKa = 4.16TSTAICRR117 pKa = 11.84LFCDD121 pKa = 3.54HH122 pKa = 7.02VEE124 pKa = 4.24AAGYY128 pKa = 7.34WAGVYY133 pKa = 10.58ASLYY137 pKa = 8.35WFQNMGDD144 pKa = 3.96LSRR147 pKa = 11.84YY148 pKa = 6.54TNWVAQWQVTACSVPTDD165 pKa = 2.8IWQFTSDD172 pKa = 4.49GIVGGISGRR181 pKa = 11.84VDD183 pKa = 3.41SNYY186 pKa = 9.58MYY188 pKa = 10.89RR189 pKa = 11.84DD190 pKa = 3.31LRR192 pKa = 11.84SVYY195 pKa = 9.03TGQVPEE201 pKa = 4.6PRR203 pKa = 11.84SEE205 pKa = 4.14VPQQATAPVSTRR217 pKa = 11.84TYY219 pKa = 9.43TVQEE223 pKa = 4.27GDD225 pKa = 3.71TLSAIAALFGTSYY238 pKa = 11.26QEE240 pKa = 3.52LAAINGITNPDD251 pKa = 4.44LIYY254 pKa = 10.24PGQVLQVTGSTQASSSTTYY273 pKa = 9.86TVEE276 pKa = 4.56SGDD279 pKa = 3.91TLSAIADD286 pKa = 3.72MYY288 pKa = 8.7GTNYY292 pKa = 9.32QHH294 pKa = 7.39LAALNGIEE302 pKa = 4.67NPDD305 pKa = 4.24LIYY308 pKa = 10.36PGQVLRR314 pKa = 11.84IEE316 pKa = 4.31

Molecular weight: 34.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AP41|A0A4D6AP41_9CAUD Putative DNA packaging protein OS=Streptococcus phage Javan100 OX=2547973 GN=Javan100_0022 PE=4 SV=1
MM1 pKa = 7.57IMTGFTKK8 pKa = 10.49RR9 pKa = 11.84LPVALIPEE17 pKa = 4.16QFLISSVRR25 pKa = 11.84KK26 pKa = 10.45DD27 pKa = 3.53MVHH30 pKa = 5.67HH31 pKa = 6.83RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84PVTIPLQAPYY44 pKa = 10.86AEE46 pKa = 4.24GMKK49 pKa = 10.1LQKK52 pKa = 9.22TKK54 pKa = 10.67PSFVPPP60 pKa = 4.03

Molecular weight: 6.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

10275

41

1097

277.7

31.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.842 ± 0.628

0.759 ± 0.139

5.382 ± 0.371

8.214 ± 0.347

3.766 ± 0.249

6.491 ± 0.284

1.81 ± 0.173

6.034 ± 0.391

7.562 ± 0.336

9.82 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.219

4.321 ± 0.206

3.017 ± 0.239

4.652 ± 0.224

4.837 ± 0.36

6.511 ± 0.322

5.83 ± 0.407

6.735 ± 0.226

1.547 ± 0.093

3.601 ± 0.353
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski