Burkholderia glumae (strain BGR1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; Burkholderia glumae

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5660 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5AN22|C5AN22_BURGB Transcriptional regulator/antitoxin MazE OS=Burkholderia glumae (strain BGR1) OX=626418 GN=bglu_1p0480 PE=4 SV=1
MM1 pKa = 6.57TTLTVGGYY9 pKa = 10.0SLGNLDD15 pKa = 5.05GISSVAAAAQQYY27 pKa = 10.1NDD29 pKa = 3.51NRR31 pKa = 11.84TTSNLINYY39 pKa = 7.3QAALANAAASATSLIPGEE57 pKa = 4.32GAAFAGNAIAANIKK71 pKa = 10.16NLSQNHH77 pKa = 4.76STMSASDD84 pKa = 3.87FEE86 pKa = 4.68SSAIAIAGALATLVGQGVEE105 pKa = 4.0ISGLAAANINPLVGGGVFTLGEE127 pKa = 4.44FINAVGFVISAAGVVIDD144 pKa = 4.83SGTIAQIATQSLQALGLAPSPSGQYY169 pKa = 10.78SPVTLSSSNFICDD182 pKa = 3.25ATNAATMWDD191 pKa = 3.43IVKK194 pKa = 10.61GDD196 pKa = 3.45NTEE199 pKa = 4.35QINSAPSNNGSGLNISNSIGGDD221 pKa = 3.21IIVAPGSPGSPYY233 pKa = 9.76TFSISGSDD241 pKa = 3.67NNIMGINSTPITGSAVGVNVSGSGNTINAGTGTSVSLSNTNGNFDD286 pKa = 3.83VVNSNGDD293 pKa = 3.19QFNQGGGISLASNTQANVNGNGNGISVANGDD324 pKa = 3.6SMGAYY329 pKa = 10.16GGGNTINAASNSLVVASSTNGAFDD353 pKa = 4.55TINTNGNTFGGTVANGQGTGVWLNQDD379 pKa = 2.45AQANVNGNNNGISVTSGDD397 pKa = 3.39SMGAYY402 pKa = 10.17GGGNTINAASNSLVVASGTNGSYY425 pKa = 9.37DD426 pKa = 3.58TINANGNSFGGTTANGQGTGVWLDD450 pKa = 3.59KK451 pKa = 11.06NVQANVNGNNIGISVTSGDD470 pKa = 3.39SMGAYY475 pKa = 10.17GGGNTINATPNSVVYY490 pKa = 10.5ASGTNGSYY498 pKa = 9.37DD499 pKa = 3.58TINANGNSFGGAAANGQGTGVWLDD523 pKa = 3.59KK524 pKa = 11.06NVQANVNGNNIGISVTSGDD543 pKa = 3.39SMGAYY548 pKa = 10.17GGGNTINATPNSVVYY563 pKa = 10.5ASGTNGSYY571 pKa = 9.37DD572 pKa = 3.58TINANGNSFGGAAANGQGTGVWLDD596 pKa = 3.59KK597 pKa = 11.06NVQANVNGNNIGISVTSGDD616 pKa = 3.39SMGAYY621 pKa = 10.17GGGNTINATPNSVVYY636 pKa = 10.5ASGTNGSYY644 pKa = 9.37DD645 pKa = 3.58TINANGNSFGGTAANGQGTGVWLDD669 pKa = 3.44KK670 pKa = 11.0NVQVNVNGAGNGIALNSGDD689 pKa = 3.79SVGVYY694 pKa = 10.6GGGNVVDD701 pKa = 4.74AAPITTTAIYY711 pKa = 7.72NTNGNAVVVNATGDD725 pKa = 3.48LFGAVTANGQGSGIWLGQNAQATVNGNGNGIGLTAGATLTATGNSDD771 pKa = 3.17IVNAAAGSVVNVSGTGDD788 pKa = 3.89EE789 pKa = 4.5INGSNSIINIAGNNEE804 pKa = 4.35TIWLSGEE811 pKa = 4.41SNVVTVTGQNVTVYY825 pKa = 10.41AQNSTVNFVGSAANNNNNKK844 pKa = 10.4LIGVKK849 pKa = 8.8NTGSGWSSNDD859 pKa = 2.21AWYY862 pKa = 10.59ALTGSSHH869 pKa = 7.59PIGATAPFQLPVFTDD884 pKa = 4.51PSASVPGDD892 pKa = 3.52NVPNATDD899 pKa = 3.81PVWIFIPYY907 pKa = 8.35TYY909 pKa = 10.31SPSGEE914 pKa = 4.6PILSEE919 pKa = 4.46PIIVTAPPDD928 pKa = 3.6VGDD931 pKa = 4.24SDD933 pKa = 5.91PIILNLNGDD942 pKa = 4.04KK943 pKa = 11.1VQTTALEE950 pKa = 4.53GSTTYY955 pKa = 11.04FDD957 pKa = 4.12MEE959 pKa = 4.35NEE961 pKa = 4.26GEE963 pKa = 4.23KK964 pKa = 10.76NQTAWGTTGEE974 pKa = 4.69GYY976 pKa = 10.55LVYY979 pKa = 10.71DD980 pKa = 4.69PEE982 pKa = 5.81DD983 pKa = 3.44VDD985 pKa = 3.66NAAVVTKK992 pKa = 10.3DD993 pKa = 3.17AQLVGGFGALQTMAQQFDD1011 pKa = 4.27GTGHH1015 pKa = 7.03GDD1017 pKa = 3.53LTVADD1022 pKa = 4.66ALWANLKK1029 pKa = 9.88VWVDD1033 pKa = 3.38KK1034 pKa = 10.82TGSGNFQSGQLMSLDD1049 pKa = 3.53QLGITSIDD1057 pKa = 3.54LDD1059 pKa = 4.06GNQTDD1064 pKa = 4.27RR1065 pKa = 11.84NSNGNKK1071 pKa = 9.86IITDD1075 pKa = 3.64SSFTRR1080 pKa = 11.84ADD1082 pKa = 3.36GSRR1085 pKa = 11.84GDD1087 pKa = 3.47IAGVDD1092 pKa = 3.76LMFSRR1097 pKa = 11.84NDD1099 pKa = 3.41TPSLVDD1105 pKa = 3.57AQVHH1109 pKa = 5.17NLISAMSLAGAAPGGQTSTGVADD1132 pKa = 4.16QNNLAALALPSHH1144 pKa = 6.97

Molecular weight: 113.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5AEN1|C5AEN1_BURGB Na+-driven multidrug efflux pump OS=Burkholderia glumae (strain BGR1) OX=626418 GN=bglu_1g11880 PE=4 SV=1
MM1 pKa = 7.98PLRR4 pKa = 11.84LPSRR8 pKa = 11.84PNRR11 pKa = 11.84AARR14 pKa = 11.84SLSRR18 pKa = 11.84LDD20 pKa = 3.38ARR22 pKa = 11.84RR23 pKa = 11.84TARR26 pKa = 11.84TLRR29 pKa = 11.84HH30 pKa = 4.77HH31 pKa = 5.71QARR34 pKa = 11.84ARR36 pKa = 11.84ALATRR41 pKa = 11.84PAAPTSRR48 pKa = 11.84LDD50 pKa = 3.56GLRR53 pKa = 11.84GWLHH57 pKa = 6.28SLLATMRR64 pKa = 11.84AHH66 pKa = 7.31AGARR70 pKa = 11.84RR71 pKa = 11.84LGLPAALLRR80 pKa = 11.84KK81 pKa = 9.28PSSRR85 pKa = 11.84RR86 pKa = 11.84HH87 pKa = 3.91AHH89 pKa = 5.15GPRR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84VNRR97 pKa = 11.84PRR99 pKa = 11.84RR100 pKa = 11.84LAASAGWFAFGTRR113 pKa = 3.72

Molecular weight: 12.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5660

0

5660

1927278

25

7806

340.5

36.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.074 ± 0.059

0.927 ± 0.01

5.499 ± 0.027

5.024 ± 0.034

3.462 ± 0.02

8.422 ± 0.035

2.306 ± 0.016

4.337 ± 0.024

2.626 ± 0.029

10.333 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.127 ± 0.014

2.522 ± 0.027

5.382 ± 0.031

3.499 ± 0.024

7.652 ± 0.045

5.403 ± 0.046

5.225 ± 0.036

7.428 ± 0.028

1.38 ± 0.013

2.371 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski