Rubella virus (strain TO-336 vaccine) (RUBV)
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q99IE7|POLN_RUBVO Non-structural polyprotein p200 OS=Rubella virus (strain TO-336 vaccine) OX=376265 PE=3 SV=1
MM1 pKa = 7.71 EE2 pKa = 5.59 KK3 pKa = 10.76 LLDD6 pKa = 3.75 EE7 pKa = 4.46 VLAPGGPYY15 pKa = 10.65 NLTVGSWVRR24 pKa = 11.84 DD25 pKa = 3.58 HH26 pKa = 6.65 VRR28 pKa = 11.84 SIVEE32 pKa = 4.2 GAWEE36 pKa = 3.97 VRR38 pKa = 11.84 DD39 pKa = 3.87 VVTAAQKK46 pKa = 9.64 RR47 pKa = 11.84 AIVAVIPRR55 pKa = 11.84 PVFTQMQVSDD65 pKa = 4.63 HH66 pKa = 6.89 PALHH70 pKa = 6.81 AISRR74 pKa = 11.84 YY75 pKa = 7.37 TRR77 pKa = 11.84 RR78 pKa = 11.84 HH79 pKa = 4.96 WIEE82 pKa = 3.82 WGPKK86 pKa = 8.11 EE87 pKa = 4.09 ALHH90 pKa = 6.11 VLIDD94 pKa = 4.06 PSPGLLRR101 pKa = 11.84 EE102 pKa = 4.03 VARR105 pKa = 11.84 VEE107 pKa = 4.17 RR108 pKa = 11.84 RR109 pKa = 11.84 WVALCLHH116 pKa = 5.78 RR117 pKa = 11.84 TARR120 pKa = 11.84 KK121 pKa = 8.85 LATALAEE128 pKa = 4.22 TASEE132 pKa = 4.08 AWHH135 pKa = 6.78 ADD137 pKa = 3.39 YY138 pKa = 11.49 VCALRR143 pKa = 11.84 GAPSGPFYY151 pKa = 10.84 VHH153 pKa = 7.22 PEE155 pKa = 4.01 DD156 pKa = 4.07 VPHH159 pKa = 7.09 GGRR162 pKa = 11.84 AVADD166 pKa = 3.92 RR167 pKa = 11.84 CLLYY171 pKa = 8.38 YY172 pKa = 10.44 TPMQMCEE179 pKa = 3.9 LMRR182 pKa = 11.84 TIDD185 pKa = 3.47 ATLLVAVDD193 pKa = 3.96 LWPVALAAHH202 pKa = 6.44 VGDD205 pKa = 4.78 DD206 pKa = 3.79 WDD208 pKa = 4.24 DD209 pKa = 4.92 LGIAWHH215 pKa = 7.03 LDD217 pKa = 3.12 HH218 pKa = 8.02 DD219 pKa = 5.09 GGCPADD225 pKa = 3.91 CRR227 pKa = 11.84 GAGAGPTPGYY237 pKa = 8.64 TRR239 pKa = 11.84 PCTTRR244 pKa = 11.84 IYY246 pKa = 10.69 QVLPDD251 pKa = 3.75 TAHH254 pKa = 7.08 PGRR257 pKa = 11.84 LYY259 pKa = 10.75 RR260 pKa = 11.84 CGPRR264 pKa = 11.84 LWTRR268 pKa = 11.84 DD269 pKa = 3.44 CAVAEE274 pKa = 4.43 LSWEE278 pKa = 4.34 VAQHH282 pKa = 6.43 CGHH285 pKa = 6.04 QARR288 pKa = 11.84 VRR290 pKa = 11.84 AVRR293 pKa = 11.84 CTLPIRR299 pKa = 11.84 HH300 pKa = 5.65 VRR302 pKa = 11.84 SLQPSARR309 pKa = 11.84 VRR311 pKa = 11.84 LPDD314 pKa = 3.8 LVHH317 pKa = 6.25 LAEE320 pKa = 4.2 VGRR323 pKa = 11.84 WRR325 pKa = 11.84 WFSLPRR331 pKa = 11.84 PVFQRR336 pKa = 11.84 MLSYY340 pKa = 10.97 CKK342 pKa = 9.59 TLSPDD347 pKa = 2.7 AYY349 pKa = 9.65 YY350 pKa = 10.61 SEE352 pKa = 5.11 RR353 pKa = 11.84 VFKK356 pKa = 10.61 FKK358 pKa = 10.86 NALSHH363 pKa = 6.94 SITLAGNVLQEE374 pKa = 4.17 GWKK377 pKa = 8.35 GTCAEE382 pKa = 4.25 EE383 pKa = 4.64 DD384 pKa = 3.6 ALCAYY389 pKa = 9.74 VAFRR393 pKa = 11.84 AWQSNARR400 pKa = 11.84 LAGIMKK406 pKa = 9.82 SAKK409 pKa = 9.66 RR410 pKa = 11.84 CAADD414 pKa = 3.49 SLSVAGWLDD423 pKa = 4.11 TIWDD427 pKa = 4.34 AIKK430 pKa = 10.52 RR431 pKa = 11.84 FFGSVPLAEE440 pKa = 5.39 RR441 pKa = 11.84 MEE443 pKa = 4.26 EE444 pKa = 4.13 WEE446 pKa = 3.68 QDD448 pKa = 3.2 AAVAAFDD455 pKa = 4.82 RR456 pKa = 11.84 GPLEE460 pKa = 4.6 DD461 pKa = 4.54 GGRR464 pKa = 11.84 HH465 pKa = 6.49 LDD467 pKa = 3.53 TVQPPKK473 pKa = 10.67 SPPRR477 pKa = 11.84 PEE479 pKa = 4.29 IAATWIVHH487 pKa = 5.91 AASADD492 pKa = 3.9 RR493 pKa = 11.84 HH494 pKa = 5.74 CACAPRR500 pKa = 11.84 RR501 pKa = 11.84 DD502 pKa = 3.87 APRR505 pKa = 11.84 EE506 pKa = 4.03 RR507 pKa = 11.84 PSAPAGPPDD516 pKa = 4.98 DD517 pKa = 4.57 EE518 pKa = 5.48 ALIPPWLFAEE528 pKa = 4.51 RR529 pKa = 11.84 RR530 pKa = 11.84 ALRR533 pKa = 11.84 CRR535 pKa = 11.84 EE536 pKa = 3.62 WDD538 pKa = 3.91 FEE540 pKa = 4.26 ALRR543 pKa = 11.84 ARR545 pKa = 11.84 ADD547 pKa = 3.53 TAAAPAPLAPRR558 pKa = 11.84 PARR561 pKa = 11.84 CPTVLYY567 pKa = 9.65 RR568 pKa = 11.84 HH569 pKa = 6.49 PAHH572 pKa = 6.22 YY573 pKa = 10.3 GPWLTLDD580 pKa = 3.8 EE581 pKa = 5.22 PGEE584 pKa = 4.15 ADD586 pKa = 3.94 AALVLCDD593 pKa = 4.92 PLGQPLRR600 pKa = 11.84 GPEE603 pKa = 3.51 RR604 pKa = 11.84 HH605 pKa = 5.75 FAAGAHH611 pKa = 5.67 MCAQARR617 pKa = 11.84 GLQAFVRR624 pKa = 11.84 VVPPPEE630 pKa = 4.46 RR631 pKa = 11.84 PWADD635 pKa = 2.87 GGARR639 pKa = 11.84 AWAKK643 pKa = 9.52 FFRR646 pKa = 11.84 GCAWAQRR653 pKa = 11.84 LLGEE657 pKa = 4.44 PAVMHH662 pKa = 6.68 LPYY665 pKa = 10.2 TDD667 pKa = 3.86 GDD669 pKa = 4.21 VPQLIALALRR679 pKa = 11.84 TLAQQGAALALSVRR693 pKa = 11.84 DD694 pKa = 4.07 LPGGAAFDD702 pKa = 4.45 ANAVTAAVRR711 pKa = 11.84 AGPGQSAATSPPPGDD726 pKa = 3.65 PPPPRR731 pKa = 11.84 RR732 pKa = 11.84 ARR734 pKa = 11.84 RR735 pKa = 11.84 SQRR738 pKa = 11.84 HH739 pKa = 4.91 LDD741 pKa = 3.56 ARR743 pKa = 11.84 GTPPPAPARR752 pKa = 11.84 DD753 pKa = 4.12 PPPPAPSPPAPPRR766 pKa = 11.84 AGDD769 pKa = 3.56 PVLPTSAGPADD780 pKa = 4.18 RR781 pKa = 11.84 ARR783 pKa = 11.84 HH784 pKa = 5.65 AEE786 pKa = 4.05 LEE788 pKa = 4.4 VAYY791 pKa = 9.7 EE792 pKa = 4.16 PSDD795 pKa = 3.66 PPTPTKK801 pKa = 10.7 ADD803 pKa = 3.78 PDD805 pKa = 3.85 SDD807 pKa = 3.51 IVEE810 pKa = 4.52 SYY812 pKa = 11.12 ARR814 pKa = 11.84 AAGPVHH820 pKa = 6.59 LRR822 pKa = 11.84 VRR824 pKa = 11.84 DD825 pKa = 3.66 IMDD828 pKa = 4.72 PPPGCKK834 pKa = 9.68 VVVNAANEE842 pKa = 4.04 GLLAGSGVCGAIFANATAALAADD865 pKa = 4.4 CRR867 pKa = 11.84 RR868 pKa = 11.84 LAPCPTGEE876 pKa = 3.96 AVATPGHH883 pKa = 5.99 GCGYY887 pKa = 6.82 THH889 pKa = 7.85 IIHH892 pKa = 6.97 AVAPRR897 pKa = 11.84 RR898 pKa = 11.84 PRR900 pKa = 11.84 DD901 pKa = 3.3 PAALEE906 pKa = 3.91 EE907 pKa = 4.8 GEE909 pKa = 4.41 ALLEE913 pKa = 3.74 RR914 pKa = 11.84 AYY916 pKa = 10.93 RR917 pKa = 11.84 SIVALAAARR926 pKa = 11.84 RR927 pKa = 11.84 WACVACPLLGAGVYY941 pKa = 9.14 GWSAAEE947 pKa = 4.07 SLRR950 pKa = 11.84 AALAATRR957 pKa = 11.84 AEE959 pKa = 3.91 PAEE962 pKa = 4.06 RR963 pKa = 11.84 VSLHH967 pKa = 5.83 ICHH970 pKa = 7.53 PDD972 pKa = 3.32 RR973 pKa = 11.84 ATLTHH978 pKa = 6.69 ASVLVGAGLAARR990 pKa = 11.84 RR991 pKa = 11.84 VSPPPTEE998 pKa = 4.4 PLASCPAGDD1007 pKa = 4.29 PGRR1010 pKa = 11.84 PAQRR1014 pKa = 11.84 SASPPATPLGDD1025 pKa = 3.31 ATAPEE1030 pKa = 3.96 PRR1032 pKa = 11.84 GCQGCEE1038 pKa = 3.34 LCRR1041 pKa = 11.84 YY1042 pKa = 7.42 TRR1044 pKa = 11.84 VTNDD1048 pKa = 3.0 RR1049 pKa = 11.84 AYY1051 pKa = 11.06 VNLWLEE1057 pKa = 4.12 RR1058 pKa = 11.84 DD1059 pKa = 3.45 RR1060 pKa = 11.84 GATSWAMRR1068 pKa = 11.84 IPEE1071 pKa = 4.12 VVVYY1075 pKa = 10.46 GPEE1078 pKa = 3.64 HH1079 pKa = 6.77 LATHH1083 pKa = 6.88 FPLNHH1088 pKa = 6.07 YY1089 pKa = 10.36 SVLKK1093 pKa = 8.84 PAEE1096 pKa = 3.98 VRR1098 pKa = 11.84 PPRR1101 pKa = 11.84 GMCGSDD1107 pKa = 2.65 MWRR1110 pKa = 11.84 CRR1112 pKa = 11.84 GWQGMPQVRR1121 pKa = 11.84 CTPSNAHH1128 pKa = 6.82 AALCRR1133 pKa = 11.84 TGVPPRR1139 pKa = 11.84 VSTRR1143 pKa = 11.84 GGEE1146 pKa = 4.12 LDD1148 pKa = 5.74 PNTCWLRR1155 pKa = 11.84 AAASVAQAARR1165 pKa = 11.84 ACGAYY1170 pKa = 9.25 TSAGCPKK1177 pKa = 9.83 CAYY1180 pKa = 9.86 GRR1182 pKa = 11.84 ALSEE1186 pKa = 4.06 ARR1188 pKa = 11.84 THH1190 pKa = 6.6 EE1191 pKa = 4.44 DD1192 pKa = 3.2 FAALSQRR1199 pKa = 11.84 WSASHH1204 pKa = 7.08 ADD1206 pKa = 3.64 ASPDD1210 pKa = 3.49 GTGDD1214 pKa = 3.63 PLDD1217 pKa = 4.33 PLMEE1221 pKa = 4.39 TVGCACSRR1229 pKa = 11.84 VWVGSEE1235 pKa = 4.02 HH1236 pKa = 6.71 EE1237 pKa = 4.68 APPDD1241 pKa = 3.45 HH1242 pKa = 6.86 LLVSLHH1248 pKa = 6.58 RR1249 pKa = 11.84 APNGPWGVVLEE1260 pKa = 4.42 VRR1262 pKa = 11.84 ARR1264 pKa = 11.84 PEE1266 pKa = 4.02 GGNPTGHH1273 pKa = 6.37 FVCAVGGGPRR1283 pKa = 11.84 RR1284 pKa = 11.84 VSDD1287 pKa = 5.06 RR1288 pKa = 11.84 PHH1290 pKa = 6.35 LWLAVPLSRR1299 pKa = 11.84 GGGTCAATDD1308 pKa = 3.42 EE1309 pKa = 4.83 GLAQAYY1315 pKa = 10.4 YY1316 pKa = 11.02 DD1317 pKa = 3.83 DD1318 pKa = 5.35 LEE1320 pKa = 4.14 VRR1322 pKa = 11.84 RR1323 pKa = 11.84 LGDD1326 pKa = 3.33 DD1327 pKa = 3.13 AMARR1331 pKa = 11.84 AALASVQRR1339 pKa = 11.84 PRR1341 pKa = 11.84 KK1342 pKa = 8.19 GPYY1345 pKa = 9.63 NIRR1348 pKa = 11.84 VWDD1351 pKa = 3.9 MAAGAGKK1358 pKa = 6.44 TTRR1361 pKa = 11.84 ILAAFTRR1368 pKa = 11.84 EE1369 pKa = 3.88 DD1370 pKa = 4.89 LYY1372 pKa = 11.75 VCPTNALLHH1381 pKa = 6.44 EE1382 pKa = 4.52 IQAKK1386 pKa = 9.83 LRR1388 pKa = 11.84 ARR1390 pKa = 11.84 DD1391 pKa = 3.4 IDD1393 pKa = 3.62 IKK1395 pKa = 11.14 NAATYY1400 pKa = 9.76 EE1401 pKa = 4.06 RR1402 pKa = 11.84 ALTKK1406 pKa = 10.32 PLAAYY1411 pKa = 9.61 RR1412 pKa = 11.84 RR1413 pKa = 11.84 IYY1415 pKa = 10.07 IDD1417 pKa = 3.12 EE1418 pKa = 4.52 AFTLGGEE1425 pKa = 4.15 YY1426 pKa = 10.46 CAFVASQTTAEE1437 pKa = 4.54 VICVGDD1443 pKa = 3.81 RR1444 pKa = 11.84 DD1445 pKa = 3.75 QCGPHH1450 pKa = 5.97 YY1451 pKa = 10.93 ANNCRR1456 pKa = 11.84 TPVPDD1461 pKa = 3.06 RR1462 pKa = 11.84 WPTEE1466 pKa = 3.98 RR1467 pKa = 11.84 SRR1469 pKa = 11.84 HH1470 pKa = 4.09 TWRR1473 pKa = 11.84 FPDD1476 pKa = 3.38 CWAARR1481 pKa = 11.84 LRR1483 pKa = 11.84 AGLDD1487 pKa = 3.21 YY1488 pKa = 10.84 DD1489 pKa = 4.04 IEE1491 pKa = 4.5 GEE1493 pKa = 4.21 RR1494 pKa = 11.84 TGTFACNLWDD1504 pKa = 3.68 GRR1506 pKa = 11.84 QVDD1509 pKa = 3.61 LHH1511 pKa = 6.44 LAFSRR1516 pKa = 11.84 EE1517 pKa = 4.05 TVRR1520 pKa = 11.84 RR1521 pKa = 11.84 LHH1523 pKa = 5.94 EE1524 pKa = 4.56 AGIRR1528 pKa = 11.84 AYY1530 pKa = 7.9 TVRR1533 pKa = 11.84 EE1534 pKa = 4.03 AQGMSVGTACIHH1546 pKa = 6.02 VGRR1549 pKa = 11.84 DD1550 pKa = 3.41 GTDD1553 pKa = 2.74 VALALTRR1560 pKa = 11.84 DD1561 pKa = 3.67 LAIVSLTRR1569 pKa = 11.84 ASDD1572 pKa = 3.29 ALYY1575 pKa = 10.56 LHH1577 pKa = 6.35 EE1578 pKa = 6.29 LEE1580 pKa = 5.76 DD1581 pKa = 4.27 GSLRR1585 pKa = 11.84 AAGLSAFLDD1594 pKa = 3.67 AGALAEE1600 pKa = 4.42 LKK1602 pKa = 9.91 EE1603 pKa = 4.4 VPAGIDD1609 pKa = 2.75 RR1610 pKa = 11.84 VVAVEE1615 pKa = 3.9 QAPPPLPPADD1625 pKa = 5.41 GIPEE1629 pKa = 4.28 AQDD1632 pKa = 3.3 VPPFCPRR1639 pKa = 11.84 TLEE1642 pKa = 3.85 EE1643 pKa = 4.42 LVFGRR1648 pKa = 11.84 AGHH1651 pKa = 5.81 PHH1653 pKa = 5.8 YY1654 pKa = 11.2 ADD1656 pKa = 3.67 LNRR1659 pKa = 11.84 VTEE1662 pKa = 4.08 GEE1664 pKa = 4.01 RR1665 pKa = 11.84 EE1666 pKa = 3.87 VRR1668 pKa = 11.84 YY1669 pKa = 9.27 MRR1671 pKa = 11.84 ISRR1674 pKa = 11.84 HH1675 pKa = 5.41 LLNKK1679 pKa = 9.92 NHH1681 pKa = 6.58 TEE1683 pKa = 3.94 MPGTEE1688 pKa = 3.61 RR1689 pKa = 11.84 VLSAVCAVRR1698 pKa = 11.84 RR1699 pKa = 11.84 YY1700 pKa = 9.74 RR1701 pKa = 11.84 AGEE1704 pKa = 4.25 DD1705 pKa = 3.34 GSTLRR1710 pKa = 11.84 TAVARR1715 pKa = 11.84 QHH1717 pKa = 6.07 PRR1719 pKa = 11.84 PFRR1722 pKa = 11.84 QIPPPRR1728 pKa = 11.84 VTAGVAQEE1736 pKa = 3.53 WRR1738 pKa = 11.84 MTYY1741 pKa = 10.01 LRR1743 pKa = 11.84 EE1744 pKa = 4.55 RR1745 pKa = 11.84 IDD1747 pKa = 3.37 LTDD1750 pKa = 3.44 VYY1752 pKa = 10.55 TQMGVAARR1760 pKa = 11.84 EE1761 pKa = 3.97 LTDD1764 pKa = 3.8 RR1765 pKa = 11.84 YY1766 pKa = 10.35 ARR1768 pKa = 11.84 RR1769 pKa = 11.84 YY1770 pKa = 8.82 PEE1772 pKa = 4.43 IFAGMCTAQSLSVPAFLKK1790 pKa = 10.13 ATLKK1794 pKa = 10.98 CVDD1797 pKa = 3.54 AALGPRR1803 pKa = 11.84 DD1804 pKa = 4.17 TEE1806 pKa = 4.3 DD1807 pKa = 2.96 CHH1809 pKa = 6.94 AAQGKK1814 pKa = 10.04 AGLEE1818 pKa = 3.43 IRR1820 pKa = 11.84 AWAKK1824 pKa = 9.92 EE1825 pKa = 3.98 WVQVMSPHH1833 pKa = 5.8 FRR1835 pKa = 11.84 AIQKK1839 pKa = 10.13 IIMRR1843 pKa = 11.84 ALRR1846 pKa = 11.84 PQFLVAAGHH1855 pKa = 5.81 TEE1857 pKa = 4.04 PEE1859 pKa = 3.7 VDD1861 pKa = 3.49 AWWQAHH1867 pKa = 4.59 YY1868 pKa = 6.67 TTNAIEE1874 pKa = 4.06 VDD1876 pKa = 4.03 FTEE1879 pKa = 5.07 FDD1881 pKa = 3.67 MNQTLATRR1889 pKa = 11.84 DD1890 pKa = 3.45 VEE1892 pKa = 4.55 LEE1894 pKa = 3.62 ISAALLGLPCAEE1906 pKa = 5.29 DD1907 pKa = 3.79 YY1908 pKa = 10.3 RR1909 pKa = 11.84 ALRR1912 pKa = 11.84 AGSYY1916 pKa = 7.78 CTLRR1920 pKa = 11.84 EE1921 pKa = 4.15 LGSTEE1926 pKa = 4.12 TGCEE1930 pKa = 3.84 RR1931 pKa = 11.84 TSGEE1935 pKa = 4.31 PATLLHH1941 pKa = 5.85 NTTVAMCMAMRR1952 pKa = 11.84 MVPKK1956 pKa = 10.0 GVRR1959 pKa = 11.84 WAGIFQGDD1967 pKa = 4.13 DD1968 pKa = 3.14 MVIFLPEE1975 pKa = 4.09 GARR1978 pKa = 11.84 SAALKK1983 pKa = 7.02 WTPAEE1988 pKa = 3.87 VGLFGFHH1995 pKa = 6.58 IPVKK1999 pKa = 10.0 HH2000 pKa = 5.99 VSTPTPSFCGHH2011 pKa = 6.39 VGTAAGLFHH2020 pKa = 7.58 DD2021 pKa = 5.18 VMHH2024 pKa = 6.08 QAIKK2028 pKa = 9.64 VLCRR2032 pKa = 11.84 RR2033 pKa = 11.84 FDD2035 pKa = 3.99 PDD2037 pKa = 3.33 VLEE2040 pKa = 4.42 EE2041 pKa = 4.05 QQVALLDD2048 pKa = 3.84 RR2049 pKa = 11.84 LRR2051 pKa = 11.84 GVYY2054 pKa = 9.61 AALPDD2059 pKa = 3.93 TVAANAAYY2067 pKa = 10.16 YY2068 pKa = 10.03 DD2069 pKa = 4.03 YY2070 pKa = 11.21 SAEE2073 pKa = 3.92 RR2074 pKa = 11.84 VLAIVRR2080 pKa = 11.84 EE2081 pKa = 4.2 LTAYY2085 pKa = 10.49 ARR2087 pKa = 11.84 GRR2089 pKa = 11.84 GLDD2092 pKa = 3.4 HH2093 pKa = 7.49 PATIGALEE2101 pKa = 4.76 EE2102 pKa = 4.08 IQTPYY2107 pKa = 11.16 ARR2109 pKa = 11.84 ANLHH2113 pKa = 6.84 DD2114 pKa = 4.79 ADD2116 pKa = 3.96
Molecular weight: 230.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.687
IPC2_protein 6.751
IPC_protein 6.678
Toseland 6.649
ProMoST 7.205
Dawson 7.22
Bjellqvist 7.512
Wikipedia 7.176
Rodwell 7.234
Grimsley 6.751
Solomon 7.22
Lehninger 7.234
Nozaki 7.834
DTASelect 7.541
Thurlkill 7.585
EMBOSS 7.556
Sillero 7.746
Patrickios 4.202
IPC_peptide 7.234
IPC2_peptide 7.454
IPC2.peptide.svr19 7.005
Protein with the highest isoelectric point:
>sp|Q99IE7|POLN_RUBVO Non-structural polyprotein p200 OS=Rubella virus (strain TO-336 vaccine) OX=376265 PE=3 SV=1
MM1 pKa = 7.88 ASTTPITMEE10 pKa = 4.54 DD11 pKa = 3.35 LQKK14 pKa = 11.25 ALEE17 pKa = 4.36 AQSRR21 pKa = 11.84 ALRR24 pKa = 11.84 AEE26 pKa = 4.19 LAAGASQSRR35 pKa = 11.84 RR36 pKa = 11.84 PRR38 pKa = 11.84 PPRR41 pKa = 11.84 QRR43 pKa = 11.84 DD44 pKa = 3.23 SSTSGDD50 pKa = 3.43 DD51 pKa = 3.11 SGRR54 pKa = 11.84 DD55 pKa = 3.08 SGGPRR60 pKa = 11.84 RR61 pKa = 11.84 RR62 pKa = 11.84 RR63 pKa = 11.84 GNRR66 pKa = 11.84 GRR68 pKa = 11.84 GQLRR72 pKa = 11.84 DD73 pKa = 3.49 WSRR76 pKa = 11.84 APPPPEE82 pKa = 3.53 EE83 pKa = 4.09 RR84 pKa = 11.84 QEE86 pKa = 4.21 SRR88 pKa = 11.84 SQTPAPKK95 pKa = 9.84 PSRR98 pKa = 11.84 APPQQPQPPRR108 pKa = 11.84 MQTGRR113 pKa = 11.84 GGSAPRR119 pKa = 11.84 PEE121 pKa = 5.32 LGPPTNPFQAAVARR135 pKa = 11.84 GLRR138 pKa = 11.84 PPLHH142 pKa = 7.23 DD143 pKa = 5.91 PDD145 pKa = 4.32 TEE147 pKa = 4.56 APTEE151 pKa = 4.1 ACVTSWLWSEE161 pKa = 4.41 GEE163 pKa = 3.99 GAVFYY168 pKa = 10.76 RR169 pKa = 11.84 VDD171 pKa = 3.36 LHH173 pKa = 5.51 FTNLGTPPLDD183 pKa = 3.76 EE184 pKa = 5.58 DD185 pKa = 4.75 GRR187 pKa = 11.84 WDD189 pKa = 3.56 PALMYY194 pKa = 9.86 NPCGPEE200 pKa = 3.78 PPAHH204 pKa = 6.01 VVRR207 pKa = 11.84 AYY209 pKa = 9.36 NQPAGDD215 pKa = 3.71 VRR217 pKa = 11.84 GVWGKK222 pKa = 9.18 GEE224 pKa = 3.9 RR225 pKa = 11.84 TYY227 pKa = 11.51 AEE229 pKa = 3.35 QDD231 pKa = 3.21 FRR233 pKa = 11.84 VGGTRR238 pKa = 11.84 WHH240 pKa = 6.89 RR241 pKa = 11.84 LLRR244 pKa = 11.84 MPVRR248 pKa = 11.84 GLDD251 pKa = 3.66 GDD253 pKa = 4.26 SAPLPPHH260 pKa = 4.04 TTEE263 pKa = 4.95 RR264 pKa = 11.84 IEE266 pKa = 4.17 TRR268 pKa = 11.84 SARR271 pKa = 11.84 HH272 pKa = 5.06 PWRR275 pKa = 11.84 IRR277 pKa = 11.84 FGAPQAFLAGLLLAAVAVGTARR299 pKa = 11.84 AGLQPRR305 pKa = 11.84 ADD307 pKa = 3.43 MAAPPTLPQPPRR319 pKa = 11.84 AHH321 pKa = 5.75 GQHH324 pKa = 6.36 YY325 pKa = 9.52 GHH327 pKa = 7.04 HH328 pKa = 6.01 HH329 pKa = 6.07 HH330 pKa = 6.44 QLPFLGHH337 pKa = 7.13 DD338 pKa = 3.65 GHH340 pKa = 7.75 HH341 pKa = 6.75 GGTLRR346 pKa = 11.84 VGQHH350 pKa = 5.88 HH351 pKa = 7.05 RR352 pKa = 11.84 NASDD356 pKa = 3.26 VLPGHH361 pKa = 6.94 WLQGGWGCYY370 pKa = 9.07 NLSDD374 pKa = 3.64 WHH376 pKa = 6.35 QGTHH380 pKa = 4.52 VCHH383 pKa = 6.52 TKK385 pKa = 10.7 HH386 pKa = 5.63 MDD388 pKa = 4.14 FWCVEE393 pKa = 3.88 HH394 pKa = 7.37 DD395 pKa = 4.24 RR396 pKa = 11.84 PPPATPTPFTTAANSTTAATPATAPAPCHH425 pKa = 6.46 AGLNDD430 pKa = 3.35 SCGGFLSGCGPMRR443 pKa = 11.84 LRR445 pKa = 11.84 HH446 pKa = 5.91 GADD449 pKa = 3.3 TRR451 pKa = 11.84 CGRR454 pKa = 11.84 LICGLSTTAQYY465 pKa = 10.77 PPTRR469 pKa = 11.84 FGCAMRR475 pKa = 11.84 WGLPPWEE482 pKa = 4.51 LVVLTARR489 pKa = 11.84 PEE491 pKa = 4.35 DD492 pKa = 3.34 GWTCRR497 pKa = 11.84 GVPAHH502 pKa = 6.99 PGTRR506 pKa = 11.84 CPEE509 pKa = 4.06 LVSPMGRR516 pKa = 11.84 ATCSPASALWLATANALSLDD536 pKa = 3.5 HH537 pKa = 7.05 ALAAFVLLVPWVLIFMVCRR556 pKa = 11.84 RR557 pKa = 11.84 ACRR560 pKa = 11.84 RR561 pKa = 11.84 RR562 pKa = 11.84 GAAAALTAVVLQGYY576 pKa = 8.06 NPPAYY581 pKa = 10.07 GEE583 pKa = 3.94 EE584 pKa = 4.14 AFTYY588 pKa = 10.53 LCTAPGCATQTPVPVRR604 pKa = 11.84 LAGVRR609 pKa = 11.84 FEE611 pKa = 4.53 SKK613 pKa = 10.26 IVDD616 pKa = 4.12 GGCFAPWDD624 pKa = 3.95 LEE626 pKa = 4.09 ATGACICEE634 pKa = 4.0 IPTDD638 pKa = 4.14 VSCEE642 pKa = 3.9 GLGAWVPTAPCARR655 pKa = 11.84 IWNGTQRR662 pKa = 11.84 ACTFWAVNAYY672 pKa = 10.54 SSGGYY677 pKa = 9.27 AQLASYY683 pKa = 8.26 FNPGGSYY690 pKa = 10.01 YY691 pKa = 10.4 KK692 pKa = 10.04 QYY694 pKa = 10.97 HH695 pKa = 5.26 PTACEE700 pKa = 3.68 VEE702 pKa = 4.34 PAFGHH707 pKa = 6.57 SDD709 pKa = 3.42 AACWGFPTDD718 pKa = 3.12 TVMSVFALASYY729 pKa = 9.01 VQHH732 pKa = 5.58 PHH734 pKa = 4.67 KK735 pKa = 9.38 TVRR738 pKa = 11.84 VKK740 pKa = 10.22 FHH742 pKa = 6.51 TEE744 pKa = 3.31 TRR746 pKa = 11.84 TVWQLSVAGASCNVTTEE763 pKa = 4.51 HH764 pKa = 7.04 PFCNTPHH771 pKa = 6.4 GQLEE775 pKa = 4.65 VQVPPDD781 pKa = 4.01 PGDD784 pKa = 3.36 LVEE787 pKa = 5.54 YY788 pKa = 10.46 IMNYY792 pKa = 9.1 TGNQQSRR799 pKa = 11.84 WGLGSPNCHH808 pKa = 5.85 GPDD811 pKa = 3.22 WASPVCQRR819 pKa = 11.84 HH820 pKa = 5.96 SPDD823 pKa = 3.14 CSRR826 pKa = 11.84 LVGATPEE833 pKa = 4.11 RR834 pKa = 11.84 PRR836 pKa = 11.84 LRR838 pKa = 11.84 LVDD841 pKa = 4.37 ADD843 pKa = 4.5 DD844 pKa = 4.4 PLLRR848 pKa = 11.84 TAPGPGEE855 pKa = 4.05 VWVTPVIGSQARR867 pKa = 11.84 KK868 pKa = 9.54 CGLHH872 pKa = 6.14 IRR874 pKa = 11.84 AGPYY878 pKa = 9.39 GHH880 pKa = 7.13 ATVEE884 pKa = 3.93 MPEE887 pKa = 4.5 WIRR890 pKa = 11.84 AHH892 pKa = 5.09 TTSDD896 pKa = 2.86 PWHH899 pKa = 6.82 PPGPLGLKK907 pKa = 9.86 FKK909 pKa = 8.93 TVRR912 pKa = 11.84 PVALPRR918 pKa = 11.84 ALAPPRR924 pKa = 11.84 NVRR927 pKa = 11.84 VTGCYY932 pKa = 9.26 QCGTPALVEE941 pKa = 4.09 GLAPGGGNCHH951 pKa = 6.28 LTLNGEE957 pKa = 4.42 DD958 pKa = 3.38 VGAFPPGKK966 pKa = 9.85 FVTAALLNTPPPYY979 pKa = 9.86 QVSCGGEE986 pKa = 3.85 SDD988 pKa = 3.51 RR989 pKa = 11.84 ASARR993 pKa = 11.84 VIDD996 pKa = 4.35 PAAQSFTGVVYY1007 pKa = 8.46 GTHH1010 pKa = 4.96 TTAVSEE1016 pKa = 4.5 TRR1018 pKa = 11.84 QTWAEE1023 pKa = 3.59 WAAAHH1028 pKa = 6.66 WWQLTLGAICALLLAGLLACCAKK1051 pKa = 10.17 CLYY1054 pKa = 9.35 YY1055 pKa = 10.72 LRR1057 pKa = 11.84 GAIAPRR1063 pKa = 3.64
Molecular weight: 114.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.545
IPC2_protein 7.571
IPC_protein 7.322
Toseland 6.956
ProMoST 8.009
Dawson 8.024
Bjellqvist 8.609
Wikipedia 7.922
Rodwell 8.039
Grimsley 7.044
Solomon 8.039
Lehninger 8.068
Nozaki 8.916
DTASelect 8.214
Thurlkill 8.258
EMBOSS 8.229
Sillero 8.639
Patrickios 4.024
IPC_peptide 8.039
IPC2_peptide 8.331
IPC2.peptide.svr19 8.223
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3179
1063
2116
1589.5
172.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.967 ± 1.306
3.303 ± 0.251
5.127 ± 0.641
5.002 ± 0.573
2.233 ± 0.167
8.147 ± 0.943
3.555 ± 0.267
2.296 ± 0.431
1.447 ± 0.122
8.367 ± 0.305
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.478 ± 0.088
1.636 ± 0.288
9.405 ± 0.822
2.957 ± 0.491
9.154 ± 0.631
4.215 ± 0.318
6.071 ± 0.742
6.637 ± 0.336
2.485 ± 0.235
2.517 ± 0.09
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here