Citrus leprosis virus N
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6AWM3|A0A1W6AWM3_9RHAB Phosphoprotein OS=Citrus leprosis virus N OX=1956177 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.55 ASSVMIDD8 pKa = 3.58 YY9 pKa = 7.67 TTVIPAYY16 pKa = 10.04 QATPTEE22 pKa = 4.17 NQVASSAPTPKK33 pKa = 10.28 QYY35 pKa = 10.28 TRR37 pKa = 11.84 AAAVAVPIVKK47 pKa = 10.0 IPPPTDD53 pKa = 3.11 NPQDD57 pKa = 3.3 LVTRR61 pKa = 11.84 FEE63 pKa = 4.53 TLMSTASSILTKK75 pKa = 10.82 AEE77 pKa = 3.69 LAEE80 pKa = 3.83 IMGIGFCITPPGMDD94 pKa = 4.09 EE95 pKa = 4.0 PAMMMVAVGAVTQGLTMGQIPATYY119 pKa = 10.05 DD120 pKa = 3.08 SRR122 pKa = 11.84 AAAANAMINIDD133 pKa = 3.84 PDD135 pKa = 3.63 EE136 pKa = 5.17 EE137 pKa = 4.29 EE138 pKa = 5.29 DD139 pKa = 5.34 KK140 pKa = 11.47 SDD142 pKa = 3.79 TPKK145 pKa = 10.88 KK146 pKa = 9.61 PAEE149 pKa = 3.92 EE150 pKa = 4.27 SAGSKK155 pKa = 9.86 AAAITYY161 pKa = 9.66 ICLSLLRR168 pKa = 11.84 LCVKK172 pKa = 10.35 EE173 pKa = 3.65 VDD175 pKa = 3.65 TFLKK179 pKa = 10.66 GVQQLKK185 pKa = 10.23 SSYY188 pKa = 8.97 HH189 pKa = 7.18 ILMGAHH195 pKa = 6.1 SEE197 pKa = 4.35 YY198 pKa = 8.35 MTSFQYY204 pKa = 10.81 SRR206 pKa = 11.84 GMCSNIASLFNQCEE220 pKa = 4.07 DD221 pKa = 3.25 LRR223 pKa = 11.84 RR224 pKa = 11.84 TLAHH228 pKa = 6.4 HH229 pKa = 6.55 CAIADD234 pKa = 3.76 EE235 pKa = 5.13 TYY237 pKa = 8.57 HH238 pKa = 5.88 QARR241 pKa = 11.84 NVHH244 pKa = 5.68 GPLRR248 pKa = 11.84 FLILQHH254 pKa = 6.3 MDD256 pKa = 3.23 LTGMIPYY263 pKa = 10.65 GMYY266 pKa = 9.83 IDD268 pKa = 5.41 LKK270 pKa = 10.37 MGLPLLSPGLLLTWLHH286 pKa = 6.74 DD287 pKa = 3.51 AHH289 pKa = 6.96 VSNVLALISEE299 pKa = 4.54 INTKK303 pKa = 10.38 YY304 pKa = 9.55 DD305 pKa = 3.21 TLTSGDD311 pKa = 3.72 RR312 pKa = 11.84 FWRR315 pKa = 11.84 YY316 pKa = 9.71 SRR318 pKa = 11.84 GIDD321 pKa = 3.15 PGFFLPLQQSKK332 pKa = 10.89 CYY334 pKa = 10.39 ILIARR339 pKa = 11.84 MADD342 pKa = 2.76 ILVRR346 pKa = 11.84 SGIVSVTQYY355 pKa = 11.13 SDD357 pKa = 3.18 PRR359 pKa = 11.84 NARR362 pKa = 11.84 CIADD366 pKa = 3.54 KK367 pKa = 8.94 TTVKK371 pKa = 10.56 AQAEE375 pKa = 4.37 IFGKK379 pKa = 9.04 EE380 pKa = 3.4 FWAAYY385 pKa = 9.79 SSLSGSSADD394 pKa = 3.56 SGPVAKK400 pKa = 9.72 TIASGSRR407 pKa = 11.84 TGNITVKK414 pKa = 10.64 RR415 pKa = 11.84 NLHH418 pKa = 6.0 PRR420 pKa = 11.84 APPPAPVVNMPPPQSSAPGALDD442 pKa = 3.76 EE443 pKa = 5.13 MVDD446 pKa = 4.42 DD447 pKa = 6.78 DD448 pKa = 4.56 EE449 pKa = 4.95 AQQ451 pKa = 3.07
Molecular weight: 48.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.451
IPC2_protein 5.461
IPC_protein 5.474
Toseland 5.779
ProMoST 5.766
Dawson 5.664
Bjellqvist 5.715
Wikipedia 5.652
Rodwell 5.639
Grimsley 5.881
Solomon 5.664
Lehninger 5.652
Nozaki 5.906
DTASelect 6.097
Thurlkill 6.033
EMBOSS 6.008
Sillero 5.995
Patrickios 3.846
IPC_peptide 5.69
IPC2_peptide 6.008
IPC2.peptide.svr19 5.976
Protein with the highest isoelectric point:
>tr|A0A1W6AWM7|A0A1W6AWM7_9RHAB Movement protein OS=Citrus leprosis virus N OX=1956177 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.29 SASSSSTLSEE11 pKa = 4.13 EE12 pKa = 4.27 CTVMSVRR19 pKa = 11.84 SRR21 pKa = 11.84 ITITSTEE28 pKa = 4.29 KK29 pKa = 10.31 IVPGPDD35 pKa = 2.48 ITAKK39 pKa = 10.3 IIQSLSLVNSKK50 pKa = 10.74 AKK52 pKa = 10.77 APDD55 pKa = 3.96 GEE57 pKa = 4.63 DD58 pKa = 3.46 VQGDD62 pKa = 3.67 WAQMLEE68 pKa = 4.37 EE69 pKa = 4.19 VAKK72 pKa = 10.64 LSKK75 pKa = 10.05 PQISGPFVDD84 pKa = 5.42 KK85 pKa = 10.97 DD86 pKa = 3.61 LEE88 pKa = 4.38 RR89 pKa = 11.84 GNIYY93 pKa = 9.92 SLNMIIGGILDD104 pKa = 3.95 SQSGRR109 pKa = 11.84 AGKK112 pKa = 10.75 VMTLRR117 pKa = 11.84 TGGLYY122 pKa = 7.68 DD123 pKa = 3.55 TPNYY127 pKa = 9.57 HH128 pKa = 6.59 INGRR132 pKa = 11.84 SLAPKK137 pKa = 9.49 INKK140 pKa = 8.88 DD141 pKa = 3.09 ASLEE145 pKa = 4.0 ITATVATVKK154 pKa = 10.6 RR155 pKa = 11.84 PVPLCKK161 pKa = 10.01 QKK163 pKa = 10.44 IQDD166 pKa = 4.03 YY167 pKa = 10.42 IILLKK172 pKa = 10.83 VVINPPTTGRR182 pKa = 11.84 GRR184 pKa = 11.84 SGG186 pKa = 2.94
Molecular weight: 19.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.732
IPC2_protein 8.521
IPC_protein 8.492
Toseland 9.297
ProMoST 8.99
Dawson 9.516
Bjellqvist 9.253
Wikipedia 9.633
Rodwell 9.867
Grimsley 9.545
Solomon 9.604
Lehninger 9.589
Nozaki 9.443
DTASelect 9.18
Thurlkill 9.385
EMBOSS 9.706
Sillero 9.502
Patrickios 5.461
IPC_peptide 9.604
IPC2_peptide 7.819
IPC2.peptide.svr19 7.803
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3606
186
1879
601.0
67.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.491 ± 0.994
1.886 ± 0.149
5.824 ± 0.214
5.241 ± 0.396
2.884 ± 0.32
6.378 ± 0.604
2.94 ± 0.34
7.404 ± 0.386
5.574 ± 0.511
8.735 ± 0.507
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.966 ± 0.149
3.966 ± 0.308
4.853 ± 0.575
2.856 ± 0.431
5.186 ± 0.719
8.93 ± 0.449
6.378 ± 0.56
6.517 ± 0.358
1.581 ± 0.206
3.411 ± 0.42
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here