Lactobacillus phage SAC12B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Tybeckvirus; Lactobacillus virus SAC12B

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 191 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5FFM5|A0A4Y5FFM5_9CAUD Uncharacterized protein OS=Lactobacillus phage SAC12B OX=2510941 GN=SAC12B_0123 PE=4 SV=1
MM1 pKa = 7.74TDD3 pKa = 2.98EE4 pKa = 4.58QYY6 pKa = 11.61EE7 pKa = 4.23FLDD10 pKa = 3.52KK11 pKa = 10.95LRR13 pKa = 11.84NALQEE18 pKa = 4.11QPEE21 pKa = 4.19EE22 pKa = 4.11PNYY25 pKa = 10.73EE26 pKa = 3.94NYY28 pKa = 10.55EE29 pKa = 4.05NGNLDD34 pKa = 3.49EE35 pKa = 6.38FYY37 pKa = 11.05DD38 pKa = 5.08LKK40 pKa = 11.25KK41 pKa = 10.26KK42 pKa = 8.47WQVVQDD48 pKa = 3.44IYY50 pKa = 11.51FLLEE54 pKa = 3.95NQDD57 pKa = 3.62YY58 pKa = 8.66MM59 pKa = 5.03

Molecular weight: 7.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5FIF1|A0A4Y5FIF1_9CAUD Uncharacterized protein OS=Lactobacillus phage SAC12B OX=2510941 GN=SAC12B_0095 PE=4 SV=1
MM1 pKa = 7.36VLLCIVSSLLILFILIFVLSYY22 pKa = 10.52LYY24 pKa = 10.43HH25 pKa = 6.57KK26 pKa = 10.06RR27 pKa = 11.84TIRR30 pKa = 11.84KK31 pKa = 8.17IEE33 pKa = 3.6KK34 pKa = 8.58RR35 pKa = 11.84NLYY38 pKa = 9.15VRR40 pKa = 11.84RR41 pKa = 11.84KK42 pKa = 9.82LNHH45 pKa = 6.13INNKK49 pKa = 8.82IVEE52 pKa = 4.13RR53 pKa = 11.84RR54 pKa = 11.84LNIEE58 pKa = 4.03QANKK62 pKa = 10.33

Molecular weight: 7.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

191

0

191

41259

31

1169

216.0

24.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.86 ± 0.191

0.572 ± 0.048

7.317 ± 0.169

5.96 ± 0.207

4.016 ± 0.109

5.662 ± 0.218

1.345 ± 0.073

7.693 ± 0.2

8.558 ± 0.242

8.304 ± 0.185

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.167 ± 0.077

7.809 ± 0.185

3.037 ± 0.126

2.979 ± 0.145

3.001 ± 0.132

8.299 ± 0.217

6.042 ± 0.234

5.936 ± 0.17

1.04 ± 0.064

5.405 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski