Kordiimonadales bacterium JCM 17845
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2941 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5A7N134|A0A5A7N134_9PROT Tryptophan synthase beta chain OS=Kordiimonadales bacterium JCM 17845 OX=1236967 GN=trpB PE=3 SV=1
MM1 pKa = 7.94 GYY3 pKa = 10.51 GLPAAIGAQLAFPDD17 pKa = 4.2 ALVIDD22 pKa = 4.3 IAGEE26 pKa = 3.74 ASIQMNIQEE35 pKa = 4.91 LATATQYY42 pKa = 11.26 RR43 pKa = 11.84 LPVKK47 pKa = 10.37 IFILNNEE54 pKa = 4.03 YY55 pKa = 10.22 MGMVRR60 pKa = 11.84 QWQEE64 pKa = 3.23 LTYY67 pKa = 9.9 EE68 pKa = 4.1 SRR70 pKa = 11.84 YY71 pKa = 9.7 SEE73 pKa = 4.71 SYY75 pKa = 10.83 SDD77 pKa = 3.82 SLPDD81 pKa = 3.79 FVKK84 pKa = 10.66 LAEE87 pKa = 4.51 AYY89 pKa = 9.23 GWTGLRR95 pKa = 11.84 ITDD98 pKa = 3.98 ASRR101 pKa = 11.84 LDD103 pKa = 5.06 DD104 pKa = 4.6 MIQTMIEE111 pKa = 4.11 TPGPVLVDD119 pKa = 3.35 CQVAQLEE126 pKa = 4.63 NCFPMVPSGAAHH138 pKa = 6.56 NEE140 pKa = 4.22 MILGPEE146 pKa = 5.0 DD147 pKa = 3.01 ISAPLSSDD155 pKa = 2.83 AQALVV160 pKa = 3.14
Molecular weight: 17.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.784
IPC2_protein 3.999
IPC_protein 3.935
Toseland 3.745
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.808
Rodwell 3.757
Grimsley 3.656
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.19
Thurlkill 3.783
EMBOSS 3.821
Sillero 4.05
Patrickios 0.947
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.934
Protein with the highest isoelectric point:
>tr|A0A5A7MYW0|A0A5A7MYW0_9PROT Molybdopterin molybdenumtransferase OS=Kordiimonadales bacterium JCM 17845 OX=1236967 GN=JCM17845_18050 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.09 QPSKK9 pKa = 8.8 IVRR12 pKa = 11.84 KK13 pKa = 9.24 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.55 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 NVLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.77 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2941
0
2941
832409
40
1629
283.0
30.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.365 ± 0.057
0.859 ± 0.015
6.419 ± 0.035
5.631 ± 0.042
3.886 ± 0.037
8.176 ± 0.042
2.236 ± 0.024
5.428 ± 0.031
3.845 ± 0.037
10.341 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.745 ± 0.021
2.807 ± 0.032
4.913 ± 0.036
3.471 ± 0.028
6.691 ± 0.037
5.831 ± 0.033
5.034 ± 0.031
6.808 ± 0.04
1.271 ± 0.016
2.24 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here