Panine betaherpesvirus 2 (Chimpanzee cytomegalovirus)
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 169 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8QRY2|Q8QRY2_9BETA Envelope protein UL131A OS=Panine betaherpesvirus 2 OX=188763 GN=UL131A PE=4 SV=1
MM1 pKa = 7.29 SLFYY5 pKa = 10.64 RR6 pKa = 11.84 IVALGALGALTWYY19 pKa = 5.51 TTNVIGEE26 pKa = 4.33 LAEE29 pKa = 4.61 GSCVANQPLEE39 pKa = 4.27 EE40 pKa = 6.15 GDD42 pKa = 3.79 DD43 pKa = 3.62 EE44 pKa = 6.39 GYY46 pKa = 11.44 GDD48 pKa = 5.7 DD49 pKa = 4.34 PALTHH54 pKa = 6.58 EE55 pKa = 4.57 DD56 pKa = 3.21 RR57 pKa = 11.84 DD58 pKa = 4.23 YY59 pKa = 10.89 RR60 pKa = 11.84 ALWAFSLVICGTLLVTCVLL79 pKa = 4.0
Molecular weight: 8.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.878
IPC2_protein 3.948
IPC_protein 3.821
Toseland 3.643
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.668
Grimsley 3.567
Solomon 3.795
Lehninger 3.745
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.757
Sillero 3.948
Patrickios 0.223
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.868
Protein with the highest isoelectric point:
>tr|Q8QRS9|Q8QRS9_9BETA Protein US31 OS=Panine betaherpesvirus 2 OX=188763 GN=US31 PE=4 SV=1
MM1 pKa = 7.44 NIEE4 pKa = 4.36 KK5 pKa = 10.39 LVNLLGLLCWIAAQTVSRR23 pKa = 11.84 IAPHH27 pKa = 6.67 ARR29 pKa = 11.84 GLSPDD34 pKa = 3.66 QLHH37 pKa = 5.92 GLYY40 pKa = 10.2 RR41 pKa = 11.84 GLHH44 pKa = 5.97 LEE46 pKa = 4.06 LLEE49 pKa = 4.17 PARR52 pKa = 11.84 PQAANRR58 pKa = 11.84 SVRR61 pKa = 11.84 DD62 pKa = 3.25 RR63 pKa = 11.84 GGPATCQEE71 pKa = 4.17 EE72 pKa = 4.39 KK73 pKa = 10.82 DD74 pKa = 3.74 RR75 pKa = 11.84 GGSFPRR81 pKa = 11.84 FSGFRR86 pKa = 11.84 ARR88 pKa = 11.84 RR89 pKa = 11.84 VPSHH93 pKa = 6.51 GSQPRR98 pKa = 11.84 GSDD101 pKa = 3.16 TLPALRR107 pKa = 11.84 FQLPEE112 pKa = 3.9 RR113 pKa = 11.84 VLLCLNRR120 pKa = 11.84 GCHH123 pKa = 5.41 GLYY126 pKa = 10.54 AVVEE130 pKa = 4.51 RR131 pKa = 11.84 PRR133 pKa = 11.84 LVGVPVSNSTVLPVLWLKK151 pKa = 10.83 PGATWEE157 pKa = 4.34 NGTAPIRR164 pKa = 11.84 GPEE167 pKa = 3.84 EE168 pKa = 3.69 RR169 pKa = 4.65
Molecular weight: 18.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.355
IPC_protein 10.379
Toseland 10.511
ProMoST 10.76
Dawson 10.613
Bjellqvist 10.423
Wikipedia 10.877
Rodwell 10.584
Grimsley 10.672
Solomon 10.804
Lehninger 10.76
Nozaki 10.57
DTASelect 10.394
Thurlkill 10.526
EMBOSS 10.935
Sillero 10.57
Patrickios 10.35
IPC_peptide 10.804
IPC2_peptide 9.882
IPC2.peptide.svr19 8.823
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
169
0
169
65757
58
2275
389.1
43.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.987 ± 0.191
2.395 ± 0.096
5.029 ± 0.114
5.478 ± 0.156
3.743 ± 0.123
6.443 ± 0.203
2.996 ± 0.099
3.435 ± 0.133
2.97 ± 0.139
10.104 ± 0.221
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.147 ± 0.081
3.187 ± 0.127
6.232 ± 0.211
3.691 ± 0.186
7.693 ± 0.19
7.798 ± 0.209
6.627 ± 0.236
7.281 ± 0.178
1.431 ± 0.087
3.33 ± 0.106
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here