Penicilliopsis zonata CBS 506.65
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9870 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L9SG18|A0A1L9SG18_9EURO Uncharacterized protein OS=Penicilliopsis zonata CBS 506.65 OX=1073090 GN=ASPZODRAFT_2071155 PE=4 SV=1
MM1 pKa = 7.76 PSVQSTLIGLTLGAALAAAAPAPSRR26 pKa = 11.84 VSEE29 pKa = 4.08 LVKK32 pKa = 10.72 KK33 pKa = 10.55 SSCTFTEE40 pKa = 4.25 AAQVSEE46 pKa = 4.7 SKK48 pKa = 10.58 SSCSTIVIDD57 pKa = 5.59 SITVPAGEE65 pKa = 4.43 TLDD68 pKa = 4.15 LTDD71 pKa = 3.87 LTEE74 pKa = 4.45 GTTVTFKK81 pKa = 10.68 GTTTWEE87 pKa = 4.04 YY88 pKa = 11.12 EE89 pKa = 4.15 EE90 pKa = 4.36 WDD92 pKa = 4.01 GPLLEE97 pKa = 4.83 ISGTKK102 pKa = 8.31 ITVNQEE108 pKa = 3.0 SDD110 pKa = 3.29 AVLDD114 pKa = 4.27 GNGAAWWDD122 pKa = 3.97 GEE124 pKa = 4.16 GSNGGKK130 pKa = 8.26 TKK132 pKa = 10.59 PKK134 pKa = 10.07 FFYY137 pKa = 10.52 AHH139 pKa = 6.64 SLDD142 pKa = 4.11 DD143 pKa = 3.93 SVISGLYY150 pKa = 9.52 IKK152 pKa = 8.11 NTPVQCFSIEE162 pKa = 4.06 SDD164 pKa = 3.12 NLLIEE169 pKa = 5.15 DD170 pKa = 3.95 VTIDD174 pKa = 5.14 DD175 pKa = 4.86 SDD177 pKa = 5.08 GDD179 pKa = 4.06 TDD181 pKa = 5.23 DD182 pKa = 5.75 LGHH185 pKa = 6.06 NTDD188 pKa = 4.0 GFDD191 pKa = 3.38 IGEE194 pKa = 4.25 STYY197 pKa = 9.71 ITITGATVYY206 pKa = 10.89 NQDD209 pKa = 3.35 DD210 pKa = 3.99 CVAINSGEE218 pKa = 3.97 NIYY221 pKa = 10.72 FSGGTCSGGHH231 pKa = 5.98 GLSIGSVGGRR241 pKa = 11.84 SDD243 pKa = 3.45 NTVKK247 pKa = 10.74 NVTFIDD253 pKa = 3.81 STVTDD258 pKa = 3.64 SEE260 pKa = 4.23 NGVRR264 pKa = 11.84 IKK266 pKa = 9.73 TVYY269 pKa = 10.39 DD270 pKa = 3.23 ATGTVEE276 pKa = 5.9 DD277 pKa = 3.94 ITYY280 pKa = 11.17 SNIKK284 pKa = 10.28 LSGISDD290 pKa = 3.74 YY291 pKa = 11.71 GLVIEE296 pKa = 4.83 QDD298 pKa = 4.03 YY299 pKa = 11.49 EE300 pKa = 4.38 NGDD303 pKa = 3.66 PTGTPSNGVTISGVTLTEE321 pKa = 3.71 ITGSVDD327 pKa = 3.16 DD328 pKa = 5.1 DD329 pKa = 3.75 AVEE332 pKa = 4.32 IYY334 pKa = 10.02 ILCGDD339 pKa = 4.84 GSCSDD344 pKa = 3.37 WTVSDD349 pKa = 3.62 VDD351 pKa = 3.34 ITGGKK356 pKa = 8.21 TSDD359 pKa = 3.09 ACEE362 pKa = 4.09 NVPSTISCTT371 pKa = 3.55
Molecular weight: 38.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.49
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.075
Thurlkill 3.554
EMBOSS 3.668
Sillero 3.846
Patrickios 0.82
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.742
Protein with the highest isoelectric point:
>tr|A0A1L9SPB4|A0A1L9SPB4_9EURO Uncharacterized protein OS=Penicilliopsis zonata CBS 506.65 OX=1073090 GN=ASPZODRAFT_13846 PE=3 SV=1
SS1 pKa = 6.85 HH2 pKa = 7.5 KK3 pKa = 10.71 SFRR6 pKa = 11.84 TKK8 pKa = 10.45 QKK10 pKa = 9.84 LAKK13 pKa = 9.55 AQKK16 pKa = 8.59 QNRR19 pKa = 11.84 PIPQWIRR26 pKa = 11.84 LRR28 pKa = 11.84 TGNTIRR34 pKa = 11.84 YY35 pKa = 5.79 NAKK38 pKa = 8.89 RR39 pKa = 11.84 RR40 pKa = 11.84 HH41 pKa = 4.14 WRR43 pKa = 11.84 KK44 pKa = 7.41 TRR46 pKa = 11.84 LGII49 pKa = 4.46
Molecular weight: 6.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9869
1
9870
4774852
49
9981
483.8
53.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.773 ± 0.028
1.268 ± 0.009
5.625 ± 0.018
6.184 ± 0.027
3.75 ± 0.016
6.716 ± 0.022
2.429 ± 0.011
4.825 ± 0.019
4.305 ± 0.025
9.339 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.136 ± 0.009
3.409 ± 0.014
5.972 ± 0.026
4.081 ± 0.017
6.259 ± 0.021
8.411 ± 0.029
6.016 ± 0.016
6.258 ± 0.018
1.462 ± 0.009
2.782 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here