Hubei sobemo-like virus 18

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE41|A0A1L3KE41_9VIRU C2H2-type domain-containing protein OS=Hubei sobemo-like virus 18 OX=1923203 PE=4 SV=1
MM1 pKa = 7.45RR2 pKa = 11.84TMIMQNFLDD11 pKa = 3.74LHH13 pKa = 6.81RR14 pKa = 11.84DD15 pKa = 3.51ARR17 pKa = 11.84WDD19 pKa = 3.5LDD21 pKa = 3.52EE22 pKa = 6.58DD23 pKa = 4.16FDD25 pKa = 4.82SFEE28 pKa = 4.2GFMRR32 pKa = 11.84SLKK35 pKa = 10.68RR36 pKa = 11.84IDD38 pKa = 4.15LSSNPGYY45 pKa = 10.08PYY47 pKa = 10.16MRR49 pKa = 11.84GCTSNRR55 pKa = 11.84QMLCYY60 pKa = 10.17KK61 pKa = 10.58NSDD64 pKa = 3.23QFDD67 pKa = 4.16PNKK70 pKa = 10.17VSILWDD76 pKa = 3.11IVRR79 pKa = 11.84QKK81 pKa = 11.39LNGSGADD88 pKa = 3.86PIKK91 pKa = 10.86LFVKK95 pKa = 10.05PEE97 pKa = 3.75PHH99 pKa = 6.54KK100 pKa = 10.61KK101 pKa = 10.38SKK103 pKa = 10.49IEE105 pKa = 3.79EE106 pKa = 3.71KK107 pKa = 10.11RR108 pKa = 11.84YY109 pKa = 10.25RR110 pKa = 11.84LISSVSLADD119 pKa = 3.39QLVDD123 pKa = 3.39HH124 pKa = 6.43MLFGKK129 pKa = 10.06MNDD132 pKa = 3.48KK133 pKa = 10.99LIASWPYY140 pKa = 10.53LPSKK144 pKa = 10.29VGWSFIGGGWKK155 pKa = 8.92TMPKK159 pKa = 9.78GGSWLAIDD167 pKa = 4.19KK168 pKa = 10.86SSWDD172 pKa = 3.16WTVQLWLLDD181 pKa = 3.71LVLQARR187 pKa = 11.84YY188 pKa = 8.59EE189 pKa = 4.16LCSTRR194 pKa = 11.84GEE196 pKa = 4.54VVDD199 pKa = 4.66RR200 pKa = 11.84WLKK203 pKa = 7.61MASMRR208 pKa = 11.84YY209 pKa = 8.39RR210 pKa = 11.84QLFKK214 pKa = 10.95EE215 pKa = 4.7PIFITSGGLLLKK227 pKa = 10.03QKK229 pKa = 10.3RR230 pKa = 11.84HH231 pKa = 4.98GVQKK235 pKa = 10.26SGCVNTIADD244 pKa = 3.81NSMMQSLLHH253 pKa = 6.35IRR255 pKa = 11.84VCLEE259 pKa = 3.81TKK261 pKa = 10.31QSVGVLYY268 pKa = 11.35SMGDD272 pKa = 3.42DD273 pKa = 3.82TLQQAPEE280 pKa = 4.04RR281 pKa = 11.84QNDD284 pKa = 3.74YY285 pKa = 11.4LSVLSKK291 pKa = 11.08YY292 pKa = 10.69CIIKK296 pKa = 10.24QADD299 pKa = 3.72ALVEE303 pKa = 3.96FAGFRR308 pKa = 11.84FDD310 pKa = 3.59GMRR313 pKa = 11.84VEE315 pKa = 4.25PVYY318 pKa = 10.62HH319 pKa = 6.59GKK321 pKa = 9.14HH322 pKa = 5.67AFNLLHH328 pKa = 7.45ADD330 pKa = 3.74PKK332 pKa = 10.95VLSDD336 pKa = 3.87LAKK339 pKa = 10.34SYY341 pKa = 10.61PLLYY345 pKa = 10.19HH346 pKa = 6.92RR347 pKa = 11.84SSNRR351 pKa = 11.84AWFRR355 pKa = 11.84EE356 pKa = 4.2LFLDD360 pKa = 3.54WHH362 pKa = 6.63GEE364 pKa = 4.23VPSIDD369 pKa = 4.05YY370 pKa = 10.78LDD372 pKa = 4.51SIYY375 pKa = 11.09DD376 pKa = 3.52GFEE379 pKa = 3.57

Molecular weight: 43.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE41|A0A1L3KE41_9VIRU C2H2-type domain-containing protein OS=Hubei sobemo-like virus 18 OX=1923203 PE=4 SV=1
MM1 pKa = 7.68EE2 pKa = 5.24PQPQVFPVEE11 pKa = 4.02EE12 pKa = 4.25GVYY15 pKa = 10.57CEE17 pKa = 4.07PTLDD21 pKa = 4.17GFVEE25 pKa = 4.71KK26 pKa = 10.1IWAKK30 pKa = 9.27IKK32 pKa = 10.56KK33 pKa = 8.79VSLVIWRR40 pKa = 11.84VWLLLVHH47 pKa = 6.72AWLYY51 pKa = 8.47YY52 pKa = 5.27TTKK55 pKa = 10.8DD56 pKa = 3.33YY57 pKa = 11.23FKK59 pKa = 10.68EE60 pKa = 4.14KK61 pKa = 10.38EE62 pKa = 4.2EE63 pKa = 4.18KK64 pKa = 10.27PEE66 pKa = 3.58VWFPRR71 pKa = 11.84LRR73 pKa = 11.84NQFNDD78 pKa = 3.09LSSILFKK85 pKa = 11.15LSLTFDD91 pKa = 4.21LEE93 pKa = 4.24WFVLVVTTTSLAFLTFVFVIKK114 pKa = 10.56RR115 pKa = 11.84SRR117 pKa = 11.84KK118 pKa = 6.4TLMMIRR124 pKa = 11.84GVRR127 pKa = 11.84FEE129 pKa = 4.53AMVEE133 pKa = 3.97GSDD136 pKa = 4.16FVKK139 pKa = 10.93GDD141 pKa = 3.15IPKK144 pKa = 10.02YY145 pKa = 9.56QVAVMKK151 pKa = 10.69AGLLRR156 pKa = 11.84DD157 pKa = 3.44VHH159 pKa = 7.82VGFGIRR165 pKa = 11.84VNNFLVVPTHH175 pKa = 6.81VINEE179 pKa = 4.13VGNAMILKK187 pKa = 9.73KK188 pKa = 10.31KK189 pKa = 10.41IKK191 pKa = 9.47IQVSMGQRR199 pKa = 11.84IISDD203 pKa = 3.77YY204 pKa = 11.4VSDD207 pKa = 4.07LTYY210 pKa = 11.08LPISNQIWADD220 pKa = 3.57LGVTKK225 pKa = 10.89SNLAKK230 pKa = 10.57EE231 pKa = 4.56SLNLAAMATIVGNGGQTTGLLRR253 pKa = 11.84KK254 pKa = 9.41CAIPFFYY261 pKa = 9.55TYY263 pKa = 10.58SASTVPGMSGAAYY276 pKa = 9.76AINNIVHH283 pKa = 7.58AIHH286 pKa = 6.63CGVVGEE292 pKa = 4.32NNSAISTVVVNKK304 pKa = 9.92EE305 pKa = 3.44LSKK308 pKa = 10.67IMKK311 pKa = 9.76GEE313 pKa = 3.9AVGATSISSNVDD325 pKa = 3.24MSWLPDD331 pKa = 3.63VEE333 pKa = 5.3NVWNDD338 pKa = 4.39DD339 pKa = 3.78LFEE342 pKa = 5.67DD343 pKa = 4.8YY344 pKa = 10.99NDD346 pKa = 3.71KK347 pKa = 10.95KK348 pKa = 10.74EE349 pKa = 4.01KK350 pKa = 10.4RR351 pKa = 11.84LIAARR356 pKa = 11.84EE357 pKa = 3.93KK358 pKa = 10.99HH359 pKa = 5.42GDD361 pKa = 3.3KK362 pKa = 10.97YY363 pKa = 11.45NPPTSGWSAMMDD375 pKa = 3.39YY376 pKa = 11.12DD377 pKa = 5.72DD378 pKa = 5.96NFAEE382 pKa = 4.55QYY384 pKa = 10.63GEE386 pKa = 4.07SSSGSKK392 pKa = 10.45SSLNVGKK399 pKa = 10.13NKK401 pKa = 10.5SPVKK405 pKa = 10.81VRR407 pKa = 11.84ITPQNNTDD415 pKa = 3.59TEE417 pKa = 4.54SEE419 pKa = 4.1IEE421 pKa = 4.65VYY423 pKa = 9.97VHH425 pKa = 6.84PADD428 pKa = 4.64AKK430 pKa = 9.65PTTKK434 pKa = 10.42SQLEE438 pKa = 4.2DD439 pKa = 3.87KK440 pKa = 10.66ISRR443 pKa = 11.84TEE445 pKa = 3.9NGSLEE450 pKa = 4.03NLHH453 pKa = 6.89LRR455 pKa = 11.84IQRR458 pKa = 11.84LEE460 pKa = 4.06GEE462 pKa = 4.29VFKK465 pKa = 10.77IAKK468 pKa = 9.43CQVCGMWVNQLSKK481 pKa = 10.45HH482 pKa = 5.54VKK484 pKa = 10.11DD485 pKa = 3.3MHH487 pKa = 6.35KK488 pKa = 10.89EE489 pKa = 3.87KK490 pKa = 10.28MSYY493 pKa = 10.34PCGKK497 pKa = 9.84CSAICRR503 pKa = 11.84SKK505 pKa = 11.15EE506 pKa = 3.63KK507 pKa = 10.36LASHH511 pKa = 6.56MLSHH515 pKa = 7.27PEE517 pKa = 3.63RR518 pKa = 11.84PKK520 pKa = 10.71CDD522 pKa = 2.94RR523 pKa = 11.84CGGFFRR529 pKa = 11.84RR530 pKa = 11.84EE531 pKa = 3.72EE532 pKa = 3.88NLVNHH537 pKa = 7.14RR538 pKa = 11.84MTCLPKK544 pKa = 10.31VRR546 pKa = 11.84FNARR550 pKa = 11.84NEE552 pKa = 4.0EE553 pKa = 4.27VVVVGEE559 pKa = 4.16SAIPSDD565 pKa = 3.87FKK567 pKa = 11.11TSVKK571 pKa = 9.99VDD573 pKa = 3.0KK574 pKa = 10.95KK575 pKa = 11.18DD576 pKa = 3.6FLGKK580 pKa = 10.18QGSQRR585 pKa = 11.84KK586 pKa = 7.89NLRR589 pKa = 11.84SSKK592 pKa = 8.97KK593 pKa = 7.86TSIASGEE600 pKa = 4.05KK601 pKa = 10.38DD602 pKa = 3.4LLTSQLEE609 pKa = 4.35FQSAMLKK616 pKa = 10.19SQDD619 pKa = 3.9AMLEE623 pKa = 4.03LLRR626 pKa = 11.84GLALGTNGQNLVTSQNN642 pKa = 3.28

Molecular weight: 72.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1021

379

642

510.5

58.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.093 ± 0.491

1.665 ± 0.046

5.877 ± 1.148

5.583 ± 0.915

4.212 ± 0.154

6.17 ± 0.091

2.253 ± 0.217

4.995 ± 0.138

8.227 ± 0.621

9.696 ± 1.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.428 ± 0.298

4.701 ± 0.864

3.722 ± 0.133

3.624 ± 0.337

4.995 ± 0.605

8.619 ± 0.198

4.016 ± 0.924

7.835 ± 1.292

2.253 ± 0.514

3.036 ± 0.519
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski