Aeromonas phage Ahp2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4ET72|A0A2H4ET72_9CAUD Putative terminase large subunit OS=Aeromonas phage Ahp2 OX=1873997 GN=Ahp2_15 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.19LDD4 pKa = 3.66SDD6 pKa = 4.18QNQTLLTFISEE17 pKa = 3.72NWEE20 pKa = 3.89QFVGHH25 pKa = 5.55VAGHH29 pKa = 6.12NGVTEE34 pKa = 4.21EE35 pKa = 4.27EE36 pKa = 4.26AEE38 pKa = 4.17EE39 pKa = 3.88LADD42 pKa = 5.05AISEE46 pKa = 4.19ALEE49 pKa = 3.87EE50 pKa = 4.03AVMYY54 pKa = 10.07DD55 pKa = 3.11

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4ET78|A0A2H4ET78_9CAUD Uncharacterized protein OS=Aeromonas phage Ahp2 OX=1873997 GN=Ahp2_74 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 10.4RR4 pKa = 11.84PQRR7 pKa = 11.84TALIATCIKK16 pKa = 9.45TGKK19 pKa = 7.52EE20 pKa = 3.58TLYY23 pKa = 10.53PSIRR27 pKa = 11.84EE28 pKa = 4.23CADD31 pKa = 2.98QGHH34 pKa = 6.57FSYY37 pKa = 10.77QYY39 pKa = 10.12VQQCLRR45 pKa = 11.84GLIPRR50 pKa = 11.84HH51 pKa = 5.81GGHH54 pKa = 6.12TFRR57 pKa = 11.84AVSGVRR63 pKa = 11.84PVEE66 pKa = 3.93KK67 pKa = 10.24VRR69 pKa = 11.84PRR71 pKa = 11.84VHH73 pKa = 7.29EE74 pKa = 3.88IAQLRR79 pKa = 11.84NSGHH83 pKa = 4.52TTAEE87 pKa = 3.53ISAITGLKK95 pKa = 9.42PDD97 pKa = 4.17TVQKK101 pKa = 9.78YY102 pKa = 7.02QKK104 pKa = 9.58QAARR108 pKa = 11.84LGITTHH114 pKa = 6.46IMPEE118 pKa = 3.84RR119 pKa = 5.05

Molecular weight: 13.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

14399

44

834

167.4

18.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.23 ± 0.519

1.382 ± 0.151

5.389 ± 0.232

6.598 ± 0.266

3.014 ± 0.154

7.931 ± 0.29

2.459 ± 0.187

5.278 ± 0.181

6.118 ± 0.305

8.521 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.292 ± 0.173

3.236 ± 0.153

4.181 ± 0.221

3.868 ± 0.156

5.987 ± 0.235

5.5 ± 0.239

5.848 ± 0.238

7.042 ± 0.27

1.827 ± 0.149

2.299 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski