Amino acid dipepetide frequency for Microbacterium phage Cressida

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.41AlaAla: 18.41 ± 1.582
0.951AlaCys: 0.951 ± 0.25
8.953AlaAsp: 8.953 ± 0.81
9.848AlaGlu: 9.848 ± 1.085
2.91AlaPhe: 2.91 ± 0.464
8.729AlaGly: 8.729 ± 0.896
2.742AlaHis: 2.742 ± 0.414
5.092AlaIle: 5.092 ± 0.558
3.134AlaLys: 3.134 ± 0.507
12.926AlaLeu: 12.926 ± 0.957
3.134AlaMet: 3.134 ± 0.416
3.301AlaAsn: 3.301 ± 0.464
7.386AlaPro: 7.386 ± 0.836
4.141AlaGln: 4.141 ± 0.472
9.177AlaArg: 9.177 ± 0.914
6.603AlaSer: 6.603 ± 0.754
8.897AlaThr: 8.897 ± 0.685
7.274AlaVal: 7.274 ± 0.727
2.686AlaTrp: 2.686 ± 0.425
2.798AlaTyr: 2.798 ± 0.374
0.0AlaXaa: 0.0 ± 0.0
Cys
1.231CysAla: 1.231 ± 0.28
0.168CysCys: 0.168 ± 0.144
0.616CysAsp: 0.616 ± 0.197
0.504CysGlu: 0.504 ± 0.167
0.112CysPhe: 0.112 ± 0.081
1.847CysGly: 1.847 ± 0.449
0.448CysHis: 0.448 ± 0.129
0.392CysIle: 0.392 ± 0.136
0.0CysLys: 0.0 ± 0.0
0.168CysLeu: 0.168 ± 0.092
0.056CysMet: 0.056 ± 0.057
0.056CysAsn: 0.056 ± 0.049
1.231CysPro: 1.231 ± 0.291
0.224CysGln: 0.224 ± 0.126
0.951CysArg: 0.951 ± 0.294
0.168CysSer: 0.168 ± 0.093
0.56CysThr: 0.56 ± 0.238
0.504CysVal: 0.504 ± 0.158
0.056CysTrp: 0.056 ± 0.051
0.392CysTyr: 0.392 ± 0.145
0.0CysXaa: 0.0 ± 0.0
Asp
9.289AspAla: 9.289 ± 0.897
0.839AspCys: 0.839 ± 0.205
5.372AspAsp: 5.372 ± 0.72
4.868AspGlu: 4.868 ± 0.63
1.175AspPhe: 1.175 ± 0.218
6.939AspGly: 6.939 ± 0.905
0.951AspHis: 0.951 ± 0.208
2.294AspIle: 2.294 ± 0.344
1.007AspLys: 1.007 ± 0.285
4.756AspLeu: 4.756 ± 0.568
0.951AspMet: 0.951 ± 0.169
1.119AspAsn: 1.119 ± 0.244
4.7AspPro: 4.7 ± 0.56
1.343AspGln: 1.343 ± 0.248
4.477AspArg: 4.477 ± 0.563
3.19AspSer: 3.19 ± 0.425
3.525AspThr: 3.525 ± 0.494
4.309AspVal: 4.309 ± 0.454
0.783AspTrp: 0.783 ± 0.209
1.679AspTyr: 1.679 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
9.401GluAla: 9.401 ± 0.736
0.783GluCys: 0.783 ± 0.207
3.078GluAsp: 3.078 ± 0.484
5.764GluGlu: 5.764 ± 0.597
1.511GluPhe: 1.511 ± 0.296
4.532GluGly: 4.532 ± 0.454
2.854GluHis: 2.854 ± 0.533
3.078GluIle: 3.078 ± 0.512
1.958GluLys: 1.958 ± 0.358
2.518GluLeu: 2.518 ± 0.399
1.903GluMet: 1.903 ± 0.355
1.455GluAsn: 1.455 ± 0.291
6.883GluPro: 6.883 ± 0.934
2.63GluGln: 2.63 ± 0.408
6.939GluArg: 6.939 ± 0.669
2.574GluSer: 2.574 ± 0.365
3.525GluThr: 3.525 ± 0.432
4.7GluVal: 4.7 ± 0.496
1.958GluTrp: 1.958 ± 0.366
1.623GluTyr: 1.623 ± 0.316
0.0GluXaa: 0.0 ± 0.0
Phe
2.798PheAla: 2.798 ± 0.35
0.224PheCys: 0.224 ± 0.103
1.847PheAsp: 1.847 ± 0.33
1.623PheGlu: 1.623 ± 0.264
0.504PhePhe: 0.504 ± 0.26
2.126PheGly: 2.126 ± 0.291
0.504PheHis: 0.504 ± 0.172
0.671PheIle: 0.671 ± 0.163
0.392PheLys: 0.392 ± 0.118
1.623PheLeu: 1.623 ± 0.252
0.28PheMet: 0.28 ± 0.125
0.504PheAsn: 0.504 ± 0.199
0.839PhePro: 0.839 ± 0.251
0.839PheGln: 0.839 ± 0.228
1.791PheArg: 1.791 ± 0.36
1.231PheSer: 1.231 ± 0.403
1.903PheThr: 1.903 ± 0.345
1.399PheVal: 1.399 ± 0.374
0.28PheTrp: 0.28 ± 0.114
0.392PheTyr: 0.392 ± 0.137
0.0PheXaa: 0.0 ± 0.0
Gly
8.785GlyAla: 8.785 ± 0.638
0.616GlyCys: 0.616 ± 0.198
6.155GlyAsp: 6.155 ± 0.592
6.323GlyGlu: 6.323 ± 0.601
2.462GlyPhe: 2.462 ± 0.337
8.449GlyGly: 8.449 ± 0.677
2.126GlyHis: 2.126 ± 0.448
3.749GlyIle: 3.749 ± 0.483
2.518GlyLys: 2.518 ± 0.426
6.379GlyLeu: 6.379 ± 1.0
2.686GlyMet: 2.686 ± 0.433
2.798GlyAsn: 2.798 ± 0.429
3.637GlyPro: 3.637 ± 0.514
2.35GlyGln: 2.35 ± 0.368
6.099GlyArg: 6.099 ± 0.592
3.805GlySer: 3.805 ± 0.778
7.666GlyThr: 7.666 ± 0.753
6.099GlyVal: 6.099 ± 0.572
2.686GlyTrp: 2.686 ± 0.404
2.798GlyTyr: 2.798 ± 0.466
0.0GlyXaa: 0.0 ± 0.0
His
2.182HisAla: 2.182 ± 0.464
0.168HisCys: 0.168 ± 0.103
1.679HisAsp: 1.679 ± 0.337
1.735HisGlu: 1.735 ± 0.412
0.28HisPhe: 0.28 ± 0.123
2.462HisGly: 2.462 ± 0.381
0.56HisHis: 0.56 ± 0.192
1.007HisIle: 1.007 ± 0.22
0.448HisLys: 0.448 ± 0.133
1.958HisLeu: 1.958 ± 0.342
0.28HisMet: 0.28 ± 0.137
0.56HisAsn: 0.56 ± 0.217
1.287HisPro: 1.287 ± 0.324
0.168HisGln: 0.168 ± 0.095
2.238HisArg: 2.238 ± 0.411
0.727HisSer: 0.727 ± 0.167
1.455HisThr: 1.455 ± 0.318
1.679HisVal: 1.679 ± 0.315
0.112HisTrp: 0.112 ± 0.076
0.448HisTyr: 0.448 ± 0.16
0.0HisXaa: 0.0 ± 0.0
Ile
6.659IleAla: 6.659 ± 0.702
0.112IleCys: 0.112 ± 0.071
3.245IleAsp: 3.245 ± 0.471
3.917IleGlu: 3.917 ± 0.442
0.671IlePhe: 0.671 ± 0.18
4.309IleGly: 4.309 ± 0.485
1.175IleHis: 1.175 ± 0.222
2.238IleIle: 2.238 ± 0.489
0.727IleLys: 0.727 ± 0.169
2.518IleLeu: 2.518 ± 0.384
0.895IleMet: 0.895 ± 0.189
1.175IleAsn: 1.175 ± 0.217
3.078IlePro: 3.078 ± 0.5
1.119IleGln: 1.119 ± 0.239
3.637IleArg: 3.637 ± 0.538
2.182IleSer: 2.182 ± 0.393
4.644IleThr: 4.644 ± 0.5
3.301IleVal: 3.301 ± 0.347
0.727IleTrp: 0.727 ± 0.217
0.671IleTyr: 0.671 ± 0.199
0.0IleXaa: 0.0 ± 0.0
Lys
4.253LysAla: 4.253 ± 0.549
0.168LysCys: 0.168 ± 0.092
0.895LysAsp: 0.895 ± 0.236
0.448LysGlu: 0.448 ± 0.159
0.616LysPhe: 0.616 ± 0.204
2.406LysGly: 2.406 ± 0.333
0.504LysHis: 0.504 ± 0.153
1.175LysIle: 1.175 ± 0.272
0.336LysLys: 0.336 ± 0.145
0.392LysLeu: 0.392 ± 0.292
0.895LysMet: 0.895 ± 0.233
0.895LysAsn: 0.895 ± 0.21
1.847LysPro: 1.847 ± 0.347
0.504LysGln: 0.504 ± 0.176
2.63LysArg: 2.63 ± 0.46
1.231LysSer: 1.231 ± 0.267
1.063LysThr: 1.063 ± 0.232
2.126LysVal: 2.126 ± 0.408
0.392LysTrp: 0.392 ± 0.144
0.336LysTyr: 0.336 ± 0.141
0.0LysXaa: 0.0 ± 0.0
Leu
9.009LeuAla: 9.009 ± 0.848
0.783LeuCys: 0.783 ± 0.232
4.532LeuAsp: 4.532 ± 0.39
3.525LeuGlu: 3.525 ± 0.47
1.567LeuPhe: 1.567 ± 0.506
6.099LeuGly: 6.099 ± 0.873
0.895LeuHis: 0.895 ± 0.26
3.973LeuIle: 3.973 ± 0.569
0.448LeuLys: 0.448 ± 0.154
5.428LeuLeu: 5.428 ± 0.689
0.839LeuMet: 0.839 ± 0.26
2.014LeuAsn: 2.014 ± 0.331
4.644LeuPro: 4.644 ± 0.546
2.126LeuGln: 2.126 ± 0.418
5.372LeuArg: 5.372 ± 0.663
4.7LeuSer: 4.7 ± 0.531
7.162LeuThr: 7.162 ± 0.656
4.756LeuVal: 4.756 ± 0.57
0.951LeuTrp: 0.951 ± 0.19
1.623LeuTyr: 1.623 ± 0.358
0.0LeuXaa: 0.0 ± 0.0
Met
2.686MetAla: 2.686 ± 0.339
0.112MetCys: 0.112 ± 0.095
1.287MetAsp: 1.287 ± 0.265
0.895MetGlu: 0.895 ± 0.222
0.28MetPhe: 0.28 ± 0.126
1.343MetGly: 1.343 ± 0.304
0.504MetHis: 0.504 ± 0.181
1.399MetIle: 1.399 ± 0.256
0.504MetLys: 0.504 ± 0.159
1.455MetLeu: 1.455 ± 0.318
0.224MetMet: 0.224 ± 0.095
0.727MetAsn: 0.727 ± 0.178
2.238MetPro: 2.238 ± 0.314
0.616MetGln: 0.616 ± 0.164
1.567MetArg: 1.567 ± 0.308
2.182MetSer: 2.182 ± 0.309
2.798MetThr: 2.798 ± 0.318
1.623MetVal: 1.623 ± 0.349
0.392MetTrp: 0.392 ± 0.117
0.448MetTyr: 0.448 ± 0.133
0.0MetXaa: 0.0 ± 0.0
Asn
3.078AsnAla: 3.078 ± 0.378
0.224AsnCys: 0.224 ± 0.112
0.951AsnAsp: 0.951 ± 0.211
1.175AsnGlu: 1.175 ± 0.269
0.392AsnPhe: 0.392 ± 0.158
3.134AsnGly: 3.134 ± 0.58
0.616AsnHis: 0.616 ± 0.202
1.007AsnIle: 1.007 ± 0.223
0.336AsnLys: 0.336 ± 0.145
1.735AsnLeu: 1.735 ± 0.376
0.336AsnMet: 0.336 ± 0.124
0.839AsnAsn: 0.839 ± 0.199
2.742AsnPro: 2.742 ± 0.496
0.168AsnGln: 0.168 ± 0.089
2.294AsnArg: 2.294 ± 0.362
1.399AsnSer: 1.399 ± 0.312
1.735AsnThr: 1.735 ± 0.303
1.847AsnVal: 1.847 ± 0.273
0.56AsnTrp: 0.56 ± 0.181
0.392AsnTyr: 0.392 ± 0.12
0.0AsnXaa: 0.0 ± 0.0
Pro
8.505ProAla: 8.505 ± 1.0
0.616ProCys: 0.616 ± 0.201
4.477ProAsp: 4.477 ± 0.528
7.33ProGlu: 7.33 ± 0.944
1.455ProPhe: 1.455 ± 0.327
5.428ProGly: 5.428 ± 0.583
1.175ProHis: 1.175 ± 0.295
2.574ProIle: 2.574 ± 0.322
1.735ProLys: 1.735 ± 0.338
3.861ProLeu: 3.861 ± 0.536
1.623ProMet: 1.623 ± 0.282
1.847ProAsn: 1.847 ± 0.269
3.749ProPro: 3.749 ± 0.578
1.735ProGln: 1.735 ± 0.613
3.245ProArg: 3.245 ± 0.441
2.854ProSer: 2.854 ± 0.463
5.652ProThr: 5.652 ± 0.644
4.085ProVal: 4.085 ± 0.423
1.007ProTrp: 1.007 ± 0.253
1.903ProTyr: 1.903 ± 0.333
0.0ProXaa: 0.0 ± 0.0
Gln
5.987GlnAla: 5.987 ± 0.572
0.392GlnCys: 0.392 ± 0.142
1.231GlnAsp: 1.231 ± 0.246
1.623GlnGlu: 1.623 ± 0.283
0.616GlnPhe: 0.616 ± 0.168
2.35GlnGly: 2.35 ± 0.357
0.783GlnHis: 0.783 ± 0.179
2.014GlnIle: 2.014 ± 0.29
0.504GlnLys: 0.504 ± 0.148
1.063GlnLeu: 1.063 ± 0.462
0.895GlnMet: 0.895 ± 0.203
0.783GlnAsn: 0.783 ± 0.287
1.063GlnPro: 1.063 ± 0.225
0.504GlnGln: 0.504 ± 0.167
1.735GlnArg: 1.735 ± 0.27
1.287GlnSer: 1.287 ± 0.239
2.182GlnThr: 2.182 ± 0.348
2.294GlnVal: 2.294 ± 0.318
0.951GlnTrp: 0.951 ± 0.235
0.951GlnTyr: 0.951 ± 0.184
0.0GlnXaa: 0.0 ± 0.0
Arg
8.505ArgAla: 8.505 ± 0.95
0.839ArgCys: 0.839 ± 0.221
5.204ArgAsp: 5.204 ± 0.633
5.316ArgGlu: 5.316 ± 0.731
2.014ArgPhe: 2.014 ± 0.362
5.987ArgGly: 5.987 ± 0.609
1.679ArgHis: 1.679 ± 0.34
3.413ArgIle: 3.413 ± 0.459
2.462ArgLys: 2.462 ± 0.483
6.883ArgLeu: 6.883 ± 0.638
2.35ArgMet: 2.35 ± 0.359
2.014ArgAsn: 2.014 ± 0.321
3.078ArgPro: 3.078 ± 0.447
2.966ArgGln: 2.966 ± 0.477
8.226ArgArg: 8.226 ± 0.926
3.134ArgSer: 3.134 ± 0.47
3.917ArgThr: 3.917 ± 0.514
4.812ArgVal: 4.812 ± 0.529
1.511ArgTrp: 1.511 ± 0.322
2.07ArgTyr: 2.07 ± 0.347
0.0ArgXaa: 0.0 ± 0.0
Ser
7.106SerAla: 7.106 ± 1.014
0.28SerCys: 0.28 ± 0.122
2.686SerAsp: 2.686 ± 0.397
2.798SerGlu: 2.798 ± 0.378
1.287SerPhe: 1.287 ± 0.282
5.316SerGly: 5.316 ± 0.871
1.175SerHis: 1.175 ± 0.223
3.022SerIle: 3.022 ± 0.41
1.287SerLys: 1.287 ± 0.304
3.805SerLeu: 3.805 ± 0.636
1.343SerMet: 1.343 ± 0.243
1.175SerAsn: 1.175 ± 0.289
2.798SerPro: 2.798 ± 0.334
1.119SerGln: 1.119 ± 0.192
3.078SerArg: 3.078 ± 0.416
2.238SerSer: 2.238 ± 0.499
3.861SerThr: 3.861 ± 0.599
4.029SerVal: 4.029 ± 0.499
0.727SerTrp: 0.727 ± 0.196
1.007SerTyr: 1.007 ± 0.266
0.0SerXaa: 0.0 ± 0.0
Thr
9.065ThrAla: 9.065 ± 0.779
1.007ThrCys: 1.007 ± 0.261
4.868ThrAsp: 4.868 ± 0.465
4.085ThrGlu: 4.085 ± 0.684
1.287ThrPhe: 1.287 ± 0.278
7.778ThrGly: 7.778 ± 0.712
1.007ThrHis: 1.007 ± 0.262
5.148ThrIle: 5.148 ± 0.586
1.958ThrLys: 1.958 ± 0.361
5.428ThrLeu: 5.428 ± 0.723
1.623ThrMet: 1.623 ± 0.264
1.063ThrAsn: 1.063 ± 0.23
6.995ThrPro: 6.995 ± 0.717
2.574ThrGln: 2.574 ± 0.373
4.532ThrArg: 4.532 ± 0.561
3.19ThrSer: 3.19 ± 0.326
6.043ThrThr: 6.043 ± 0.623
6.379ThrVal: 6.379 ± 0.855
1.455ThrTrp: 1.455 ± 0.259
1.175ThrTyr: 1.175 ± 0.266
0.0ThrXaa: 0.0 ± 0.0
Val
7.442ValAla: 7.442 ± 0.755
0.783ValCys: 0.783 ± 0.225
3.861ValAsp: 3.861 ± 0.47
4.141ValGlu: 4.141 ± 0.447
1.511ValPhe: 1.511 ± 0.382
5.652ValGly: 5.652 ± 0.651
1.287ValHis: 1.287 ± 0.329
3.134ValIle: 3.134 ± 0.46
2.182ValLys: 2.182 ± 0.333
4.532ValLeu: 4.532 ± 0.497
1.679ValMet: 1.679 ± 0.299
1.399ValAsn: 1.399 ± 0.24
4.365ValPro: 4.365 ± 0.484
2.35ValGln: 2.35 ± 0.342
5.148ValArg: 5.148 ± 0.581
4.253ValSer: 4.253 ± 0.494
7.386ValThr: 7.386 ± 0.68
4.029ValVal: 4.029 ± 0.445
1.007ValTrp: 1.007 ± 0.245
1.903ValTyr: 1.903 ± 0.283
0.0ValXaa: 0.0 ± 0.0
Trp
1.735TrpAla: 1.735 ± 0.3
0.392TrpCys: 0.392 ± 0.133
1.063TrpAsp: 1.063 ± 0.239
1.791TrpGlu: 1.791 ± 0.289
0.56TrpPhe: 0.56 ± 0.174
1.007TrpGly: 1.007 ± 0.23
0.336TrpHis: 0.336 ± 0.12
0.448TrpIle: 0.448 ± 0.187
0.783TrpLys: 0.783 ± 0.202
1.511TrpLeu: 1.511 ± 0.309
0.504TrpMet: 0.504 ± 0.173
0.783TrpAsn: 0.783 ± 0.21
1.063TrpPro: 1.063 ± 0.271
1.063TrpGln: 1.063 ± 0.23
1.287TrpArg: 1.287 ± 0.264
1.623TrpSer: 1.623 ± 0.292
1.511TrpThr: 1.511 ± 0.249
1.287TrpVal: 1.287 ± 0.293
0.504TrpTrp: 0.504 ± 0.179
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.966TyrAla: 2.966 ± 0.435
0.224TyrCys: 0.224 ± 0.11
1.567TyrAsp: 1.567 ± 0.315
1.958TyrGlu: 1.958 ± 0.327
0.56TyrPhe: 0.56 ± 0.206
2.35TyrGly: 2.35 ± 0.341
0.112TyrHis: 0.112 ± 0.079
1.175TyrIle: 1.175 ± 0.282
0.392TyrLys: 0.392 ± 0.143
1.511TyrLeu: 1.511 ± 0.274
0.727TyrMet: 0.727 ± 0.175
0.448TyrAsn: 0.448 ± 0.164
1.231TyrPro: 1.231 ± 0.248
0.671TyrGln: 0.671 ± 0.193
1.958TyrArg: 1.958 ± 0.328
1.567TyrSer: 1.567 ± 0.363
1.175TyrThr: 1.175 ± 0.287
1.511TyrVal: 1.511 ± 0.347
0.504TyrTrp: 0.504 ± 0.156
0.671TyrTyr: 0.671 ± 0.164
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (17872 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski