Circoviridae sp. ctTgd7

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W8P2|A0A5Q2W8P2_9CIRC Rep protein OS=Circoviridae sp. ctTgd7 OX=2656727 PE=4 SV=1
MM1 pKa = 7.53TSRR4 pKa = 11.84NYY6 pKa = 11.01CMTIWDD12 pKa = 4.72DD13 pKa = 4.01NFSEE17 pKa = 5.1ASMDD21 pKa = 3.58LSKK24 pKa = 10.61IRR26 pKa = 11.84YY27 pKa = 8.73CVFQRR32 pKa = 11.84EE33 pKa = 4.41KK34 pKa = 11.1APQTGKK40 pKa = 9.98LHH42 pKa = 4.87YY43 pKa = 9.51QGYY46 pKa = 9.54VEE48 pKa = 4.46LFKK51 pKa = 10.38PARR54 pKa = 11.84HH55 pKa = 6.2GGLKK59 pKa = 8.91TALGCSSLWAKK70 pKa = 9.82PRR72 pKa = 11.84AGSRR76 pKa = 11.84DD77 pKa = 3.35EE78 pKa = 4.18ARR80 pKa = 11.84AYY82 pKa = 10.48CMKK85 pKa = 10.67EE86 pKa = 3.71EE87 pKa = 4.18TRR89 pKa = 11.84VEE91 pKa = 4.46GPWEE95 pKa = 3.94VGTWISGPGARR106 pKa = 11.84TDD108 pKa = 3.74LEE110 pKa = 4.38NVVDD114 pKa = 4.55MIKK117 pKa = 10.46AGRR120 pKa = 11.84PKK122 pKa = 10.67EE123 pKa = 4.28EE124 pKa = 4.34IFSEE128 pKa = 4.47YY129 pKa = 10.14PEE131 pKa = 4.47TYY133 pKa = 10.39LRR135 pKa = 11.84FHH137 pKa = 6.77GGIDD141 pKa = 3.12KK142 pKa = 10.99ALAFQQKK149 pKa = 8.71PRR151 pKa = 11.84LRR153 pKa = 11.84EE154 pKa = 3.9GLEE157 pKa = 3.91VKK159 pKa = 9.83VWWGVPGAGKK169 pKa = 6.84THH171 pKa = 7.74RR172 pKa = 11.84ARR174 pKa = 11.84EE175 pKa = 3.99WLGDD179 pKa = 5.24DD180 pKa = 3.55IYY182 pKa = 11.21EE183 pKa = 4.14WPAKK187 pKa = 10.24CEE189 pKa = 3.81EE190 pKa = 3.8PRR192 pKa = 11.84GYY194 pKa = 10.5KK195 pKa = 10.14GQSCVLIDD203 pKa = 4.78EE204 pKa = 4.7FSGWLPASLFKK215 pKa = 10.63TITDD219 pKa = 3.23KK220 pKa = 10.75WPCVVKK226 pKa = 10.71VPYY229 pKa = 10.31GQVAWVPEE237 pKa = 4.41KK238 pKa = 10.64IAITSNFDD246 pKa = 3.11PVTWYY251 pKa = 10.74NNKK254 pKa = 9.36VDD256 pKa = 3.76TQAVLRR262 pKa = 11.84RR263 pKa = 11.84FTEE266 pKa = 3.9VTACVTRR273 pKa = 11.84YY274 pKa = 9.21EE275 pKa = 4.26VTGNKK280 pKa = 9.82LPP282 pKa = 3.91

Molecular weight: 32.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W8P2|A0A5Q2W8P2_9CIRC Rep protein OS=Circoviridae sp. ctTgd7 OX=2656727 PE=4 SV=1
MM1 pKa = 7.63PPRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84SSKK9 pKa = 7.67YY10 pKa = 8.61KK11 pKa = 9.43RR12 pKa = 11.84YY13 pKa = 9.98RR14 pKa = 11.84RR15 pKa = 11.84FKK17 pKa = 9.05RR18 pKa = 11.84AYY20 pKa = 9.46RR21 pKa = 11.84KK22 pKa = 10.33GGVWGLAKK30 pKa = 10.18KK31 pKa = 9.99AASSVVKK38 pKa = 10.39YY39 pKa = 9.96YY40 pKa = 11.14LNPEE44 pKa = 4.07YY45 pKa = 10.95KK46 pKa = 10.46FLDD49 pKa = 3.35SAANLNPTNSGNIVSVQSLIAYY71 pKa = 9.4GDD73 pKa = 3.61AEE75 pKa = 4.25NNRR78 pKa = 11.84NGNSIKK84 pKa = 9.16VTSMLYY90 pKa = 10.24RR91 pKa = 11.84ATATLGTGATSTKK104 pKa = 8.6YY105 pKa = 10.4RR106 pKa = 11.84IMIFTDD112 pKa = 3.63TSSAGAVPTVNTVLQAPGGANPLILAPLNRR142 pKa = 11.84NNGSRR147 pKa = 11.84FHH149 pKa = 6.88VLLDD153 pKa = 3.16RR154 pKa = 11.84TYY156 pKa = 10.99ILDD159 pKa = 3.38QDD161 pKa = 4.25DD162 pKa = 4.41PKK164 pKa = 11.53KK165 pKa = 10.27HH166 pKa = 5.16IQIFKK171 pKa = 10.56KK172 pKa = 9.2MNHH175 pKa = 6.84HH176 pKa = 5.71IHH178 pKa = 6.13YY179 pKa = 10.1LDD181 pKa = 3.4GTAAVTALGQGPIYY195 pKa = 10.25IMHH198 pKa = 7.1ISDD201 pKa = 3.58EE202 pKa = 4.29ATNYY206 pKa = 7.38PTVDD210 pKa = 2.97WNCRR214 pKa = 11.84LRR216 pKa = 11.84FLDD219 pKa = 3.54NN220 pKa = 3.52

Molecular weight: 24.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

502

220

282

251.0

28.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.968 ± 0.695

1.793 ± 0.829

4.781 ± 0.136

5.179 ± 2.362

3.187 ± 0.285

7.57 ± 0.465

1.992 ± 0.455

4.781 ± 0.698

6.972 ± 0.377

6.972 ± 0.749

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.992 ± 0.174

4.98 ± 1.982

5.578 ± 0.358

2.59 ± 0.196

6.972 ± 0.186

5.578 ± 0.768

6.773 ± 0.309

6.574 ± 0.693

2.789 ± 1.164

4.98 ± 0.575
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski