Terrisporobacter othiniensis
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3449 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B3VTF9|A0A0B3VTF9_9FIRM Uncharacterized protein OS=Terrisporobacter othiniensis OX=1577792 GN=QX51_16810 PE=3 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.48 AHH4 pKa = 6.97 VDD6 pKa = 3.26 RR7 pKa = 11.84 DD8 pKa = 3.79 TCIGCGVCPSVCPAVFEE25 pKa = 4.38 MDD27 pKa = 4.56 DD28 pKa = 3.59 EE29 pKa = 4.79 GLSHH33 pKa = 7.02 VIVDD37 pKa = 4.2 TVPADD42 pKa = 3.81 AEE44 pKa = 4.5 DD45 pKa = 4.01 DD46 pKa = 3.91 AKK48 pKa = 11.04 DD49 pKa = 3.73 AEE51 pKa = 4.63 EE52 pKa = 4.45 SCPVDD57 pKa = 4.55 AITVEE62 pKa = 4.11
Molecular weight: 6.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.77
IPC_protein 3.719
Toseland 3.503
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.948
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.427
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.075
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.846
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|A0A0B3VWW8|A0A0B3VWW8_9FIRM Transcription antiterminator LicT OS=Terrisporobacter othiniensis OX=1577792 GN=QX51_08635 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 9.0 KK9 pKa = 7.87 RR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 8.39 KK14 pKa = 8.49 EE15 pKa = 3.5 HH16 pKa = 6.11 GFRR19 pKa = 11.84 KK20 pKa = 9.77 RR21 pKa = 11.84 MKK23 pKa = 9.11 TSNGRR28 pKa = 11.84 NVLKK32 pKa = 10.5 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.35 GRR39 pKa = 11.84 NRR41 pKa = 11.84 LTHH44 pKa = 6.18
Molecular weight: 5.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 10.877
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.017
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3449
0
3449
1029502
37
1994
298.5
33.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.487 ± 0.045
1.349 ± 0.017
5.746 ± 0.037
7.421 ± 0.053
4.292 ± 0.035
6.413 ± 0.043
1.344 ± 0.015
9.961 ± 0.046
8.951 ± 0.046
9.032 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.775 ± 0.023
6.386 ± 0.04
2.723 ± 0.023
2.239 ± 0.017
3.088 ± 0.025
6.351 ± 0.038
4.873 ± 0.033
6.722 ± 0.038
0.596 ± 0.013
4.25 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here