Gossypium darwinii symptomless virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Cotton leaf curl Multan virus

Average proteome isoelectric point is 7.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8Q6Q7|B8Q6Q7_9GEMI AC4 OS=Gossypium darwinii symptomless virus OX=509531 GN=AC4 PE=3 SV=1
MM1 pKa = 7.43PSKK4 pKa = 10.64RR5 pKa = 11.84FQIYY9 pKa = 9.59SKK11 pKa = 10.96NYY13 pKa = 7.65FLTYY17 pKa = 9.19PKK19 pKa = 10.53CSLTKK24 pKa = 10.66EE25 pKa = 4.11EE26 pKa = 5.14ALSQIQNLQTPTNKK40 pKa = 9.9KK41 pKa = 9.7FIKK44 pKa = 9.59ICKK47 pKa = 8.28EE48 pKa = 3.47LHH50 pKa = 6.41EE51 pKa = 4.9NGEE54 pKa = 4.16PHH56 pKa = 6.66LHH58 pKa = 6.03VLIQFEE64 pKa = 4.78GKK66 pKa = 9.95YY67 pKa = 9.77KK68 pKa = 10.46CQNQRR73 pKa = 11.84FFDD76 pKa = 4.04LVSPTRR82 pKa = 11.84SAHH85 pKa = 4.86FHH87 pKa = 6.55PNIQGAKK94 pKa = 9.4SSSDD98 pKa = 3.13VKK100 pKa = 11.13DD101 pKa = 4.27YY102 pKa = 11.02IDD104 pKa = 4.6KK105 pKa = 11.38DD106 pKa = 3.5GDD108 pKa = 3.68TLEE111 pKa = 4.14WGEE114 pKa = 3.84FQIDD118 pKa = 3.21GRR120 pKa = 11.84SARR123 pKa = 11.84GGQQTANDD131 pKa = 4.05AYY133 pKa = 10.69AAALNAGSKK142 pKa = 10.29SEE144 pKa = 3.84ALRR147 pKa = 11.84VIKK150 pKa = 10.2EE151 pKa = 3.88LAPKK155 pKa = 10.6DD156 pKa = 3.7FVLQFHH162 pKa = 6.76NLNANQSKK170 pKa = 10.22IFQEE174 pKa = 4.35PPAPYY179 pKa = 9.18ISPFSRR185 pKa = 11.84SSFDD189 pKa = 3.35QVPEE193 pKa = 3.95EE194 pKa = 4.36LEE196 pKa = 3.76VWAIDD201 pKa = 4.05NVVDD205 pKa = 4.08PAARR209 pKa = 11.84PLRR212 pKa = 11.84PRR214 pKa = 11.84SIVIEE219 pKa = 4.26GDD221 pKa = 3.21SRR223 pKa = 11.84TGKK226 pKa = 8.52TMWARR231 pKa = 11.84SLGPHH236 pKa = 6.71NYY238 pKa = 10.18LCGHH242 pKa = 7.38LDD244 pKa = 4.18LSPKK248 pKa = 10.15VYY250 pKa = 11.07SNDD253 pKa = 2.25AWYY256 pKa = 10.92NVIDD260 pKa = 4.79DD261 pKa = 4.33VDD263 pKa = 3.73PHH265 pKa = 6.08FLKK268 pKa = 10.67HH269 pKa = 5.9FKK271 pKa = 10.69EE272 pKa = 4.43FMGAQRR278 pKa = 11.84DD279 pKa = 3.81WQSNTKK285 pKa = 9.6YY286 pKa = 10.57GKK288 pKa = 9.12PVQIKK293 pKa = 10.41GGIPTIFLCNPGPHH307 pKa = 6.2SSYY310 pKa = 11.39KK311 pKa = 10.58EE312 pKa = 3.76FLDD315 pKa = 3.97EE316 pKa = 4.83EE317 pKa = 4.8KK318 pKa = 10.25NTALKK323 pKa = 10.38NWAVKK328 pKa = 10.17NAIFITLEE336 pKa = 4.07GPLYY340 pKa = 10.88SGTNQSTAQGSEE352 pKa = 3.9EE353 pKa = 3.7AHH355 pKa = 5.77QEE357 pKa = 4.01EE358 pKa = 5.21EE359 pKa = 4.37SRR361 pKa = 11.84SS362 pKa = 3.67

Molecular weight: 41.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8Q6Q4|B8Q6Q4_9GEMI Replication enhancer OS=Gossypium darwinii symptomless virus OX=509531 GN=AC3 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 10.21RR4 pKa = 11.84AADD7 pKa = 3.55IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFGSPYY27 pKa = 7.75TNRR30 pKa = 11.84VAAPIVRR37 pKa = 11.84VTKK40 pKa = 9.91QQAWTNRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.8PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.57RR60 pKa = 11.84SPDD63 pKa = 3.29VPKK66 pKa = 10.81GCEE69 pKa = 4.39GPCKK73 pKa = 10.05VQSFEE78 pKa = 4.02SRR80 pKa = 11.84HH81 pKa = 5.54DD82 pKa = 3.51VVHH85 pKa = 6.75IGKK88 pKa = 9.41VMCISDD94 pKa = 3.38VMRR97 pKa = 11.84GVGLTHH103 pKa = 7.69RR104 pKa = 11.84IGKK107 pKa = 8.54RR108 pKa = 11.84FCVKK112 pKa = 9.96SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.3KK148 pKa = 10.52PHH150 pKa = 7.78DD151 pKa = 4.09FGEE154 pKa = 4.35VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.57NSHH172 pKa = 6.59RR173 pKa = 11.84DD174 pKa = 3.15RR175 pKa = 11.84YY176 pKa = 9.17QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.68KK201 pKa = 9.39FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.95ATLKK245 pKa = 10.04SPIYY249 pKa = 10.32FYY251 pKa = 10.95DD252 pKa = 3.68YY253 pKa = 10.98VANN256 pKa = 4.0

Molecular weight: 29.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1123

100

362

187.2

21.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.076 ± 0.86

2.315 ± 0.644

4.63 ± 0.391

4.898 ± 0.861

4.274 ± 0.447

4.987 ± 0.325

3.651 ± 0.268

4.63 ± 0.566

6.233 ± 0.913

7.302 ± 1.153

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.226 ± 0.624

6.055 ± 0.533

5.877 ± 0.577

5.788 ± 0.871

6.768 ± 1.028

8.37 ± 1.211

5.432 ± 0.894

6.055 ± 1.26

1.336 ± 0.118

4.096 ± 0.554
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski