Nocardioides iriomotensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4477 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1Z0W4|A0A4V1Z0W4_9ACTN DUF4386 domain-containing protein OS=Nocardioides iriomotensis OX=715784 GN=ETU37_22405 PE=4 SV=1
MM1 pKa = 6.84NRR3 pKa = 11.84KK4 pKa = 5.51WTAAAAAVSGMVLTLSACGGGADD27 pKa = 3.94GTSAGGDD34 pKa = 3.72YY35 pKa = 10.53PSEE38 pKa = 4.37DD39 pKa = 4.1MDD41 pKa = 3.32WTIAFGPGGGNDD53 pKa = 2.59IMARR57 pKa = 11.84TMVDD61 pKa = 2.86ILQKK65 pKa = 10.79NDD67 pKa = 4.05LYY69 pKa = 10.71PEE71 pKa = 4.52NIVVEE76 pKa = 4.3NKK78 pKa = 10.13EE79 pKa = 4.24GGSGATGWGYY89 pKa = 11.26LFGQSGTGYY98 pKa = 10.71GISTTSGSFITTPLQADD115 pKa = 4.11TGWEE119 pKa = 4.08PTDD122 pKa = 3.76FTPVGLMAADD132 pKa = 3.78YY133 pKa = 10.62ALLMTAGDD141 pKa = 4.84GEE143 pKa = 4.39WSTYY147 pKa = 9.87DD148 pKa = 3.04EE149 pKa = 4.13WVEE152 pKa = 3.86YY153 pKa = 10.34AKK155 pKa = 10.95SKK157 pKa = 9.24GTVAVGGIGTVNVDD171 pKa = 3.72YY172 pKa = 10.72IVQQMVADD180 pKa = 4.06ANGYY184 pKa = 9.53KK185 pKa = 9.15IDD187 pKa = 3.7YY188 pKa = 9.91VPYY191 pKa = 9.78NEE193 pKa = 4.44EE194 pKa = 3.86GQMQTSLLSGAIDD207 pKa = 3.99AMISNPGSIMGQIEE221 pKa = 4.47GGQMNALLFTGPEE234 pKa = 3.98RR235 pKa = 11.84LDD237 pKa = 3.71ALADD241 pKa = 3.54VPTAEE246 pKa = 4.81EE247 pKa = 4.44EE248 pKa = 4.78GISDD252 pKa = 3.89IPSMPRR258 pKa = 11.84GLILPPDD265 pKa = 4.16APEE268 pKa = 4.68EE269 pKa = 4.03AQQWWIDD276 pKa = 3.49TMKK279 pKa = 10.74EE280 pKa = 4.13VVTTPEE286 pKa = 3.65WQDD289 pKa = 3.62YY290 pKa = 11.09LDD292 pKa = 4.05EE293 pKa = 5.04NYY295 pKa = 10.74LLKK298 pKa = 10.72DD299 pKa = 3.98EE300 pKa = 4.68KK301 pKa = 10.42WGEE304 pKa = 4.17DD305 pKa = 3.21FTAYY309 pKa = 10.89LDD311 pKa = 3.89DD312 pKa = 4.11TQSSFEE318 pKa = 4.11EE319 pKa = 4.08QLTEE323 pKa = 5.06LGALL327 pKa = 3.75

Molecular weight: 35.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q5J3I6|A0A4Q5J3I6_9ACTN MaoC-like domain-containing protein OS=Nocardioides iriomotensis OX=715784 GN=ETU37_12315 PE=3 SV=1
MM1 pKa = 7.56PARR4 pKa = 11.84RR5 pKa = 11.84HH6 pKa = 4.53GTHH9 pKa = 7.02PRR11 pKa = 11.84RR12 pKa = 11.84APRR15 pKa = 11.84AAAPRR20 pKa = 11.84SPARR24 pKa = 11.84RR25 pKa = 11.84PARR28 pKa = 11.84GARR31 pKa = 11.84RR32 pKa = 11.84PGRR35 pKa = 11.84GPVPSSWHH43 pKa = 5.51PRR45 pKa = 11.84PARR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84SPPAGRR57 pKa = 11.84RR58 pKa = 11.84PPRR61 pKa = 4.22

Molecular weight: 6.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4477

0

4477

1426832

26

1908

318.7

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.957 ± 0.046

0.721 ± 0.01

6.768 ± 0.032

5.633 ± 0.033

2.829 ± 0.022

9.226 ± 0.035

2.268 ± 0.018

3.037 ± 0.025

1.95 ± 0.031

10.323 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.831 ± 0.014

1.704 ± 0.022

5.643 ± 0.026

2.668 ± 0.018

7.863 ± 0.043

5.08 ± 0.025

6.117 ± 0.029

9.815 ± 0.043

1.563 ± 0.018

2.005 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski