Nocardia cyriacigeorgica (strain GUH-2)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia; Nocardia cyriacigeorgica

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5473 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6R6R1|H6R6R1_NOCCG Putative Nucleoside diphosphate kinase OS=Nocardia cyriacigeorgica (strain GUH-2) OX=1127134 GN=NOCYR_1428 PE=3 SV=1
MM1 pKa = 7.11GVEE4 pKa = 4.78FVSDD8 pKa = 3.23TGIGDD13 pKa = 3.6GGVGGFGDD21 pKa = 4.46GGAGGDD27 pKa = 3.28AGFGGGADD35 pKa = 3.77SGTTDD40 pKa = 3.28SGVGGLGDD48 pKa = 3.92SGTSGGDD55 pKa = 3.02SGLGSGLGDD64 pKa = 3.32GGFGGGATVPGLPTDD79 pKa = 3.75SLPDD83 pKa = 3.61LGGDD87 pKa = 3.66SGIGTIPGTGEE98 pKa = 4.19TPQLPAPPSDD108 pKa = 3.9SDD110 pKa = 3.58PGQPVPADD118 pKa = 3.44PVHH121 pKa = 6.25PHH123 pKa = 7.05DD124 pKa = 5.92PNGGWHH130 pKa = 6.67GDD132 pKa = 2.97RR133 pKa = 11.84WPFPDD138 pKa = 4.1PGYY141 pKa = 9.69PFSPFFPQTPSPEE154 pKa = 4.07PLEE157 pKa = 5.8DD158 pKa = 4.36FDD160 pKa = 5.18PPCAPGQRR168 pKa = 11.84GAHH171 pKa = 6.85CDD173 pKa = 3.06TDD175 pKa = 4.75LFDD178 pKa = 3.64GHH180 pKa = 6.85TGQSAPLTPAPSPGTHH196 pKa = 6.57HH197 pKa = 7.13GLPFGLSLPSGSAGQLL213 pKa = 3.25

Molecular weight: 20.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6R3G6|H6R3G6_NOCCG Uncharacterized protein OS=Nocardia cyriacigeorgica (strain GUH-2) OX=1127134 GN=NOCYR_1115 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5473

0

5473

1788460

9

14562

326.8

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.57 ± 0.049

0.713 ± 0.011

6.269 ± 0.035

5.603 ± 0.025

2.899 ± 0.022

8.895 ± 0.033

2.183 ± 0.019

4.206 ± 0.023

1.957 ± 0.025

10.035 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.927 ± 0.016

1.969 ± 0.015

6.038 ± 0.039

2.915 ± 0.018

7.66 ± 0.036

5.1 ± 0.02

6.154 ± 0.029

8.39 ± 0.037

1.445 ± 0.015

2.071 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski