Plantago asiatica mosaic potexvirus (P1AMV)
Average proteome isoelectric point is 7.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q07519|TGB2_P1AMV Movement protein TGB2 OS=Plantago asiatica mosaic potexvirus OX=28354 GN=ORF3 PE=3 SV=1
MM1 pKa = 7.36 SNVRR5 pKa = 11.84 NVFSQLTDD13 pKa = 3.73 PSLKK17 pKa = 10.67 AVIQDD22 pKa = 3.04 EE23 pKa = 4.7 AYY25 pKa = 9.93 RR26 pKa = 11.84 VLKK29 pKa = 10.79 RR30 pKa = 11.84 EE31 pKa = 4.11 LAQTRR36 pKa = 11.84 HH37 pKa = 5.62 TNPFAQSPEE46 pKa = 3.88 AADD49 pKa = 3.47 ALEE52 pKa = 4.01 ALGINSHH59 pKa = 7.12 PYY61 pKa = 10.07 AITSHH66 pKa = 4.72 THH68 pKa = 5.37 AAAKK72 pKa = 10.3 AIEE75 pKa = 4.19 SDD77 pKa = 3.32 LYY79 pKa = 11.07 EE80 pKa = 4.48 VVSHH84 pKa = 6.29 YY85 pKa = 10.61 LPKK88 pKa = 10.33 EE89 pKa = 3.99 NPVSFLFMKK98 pKa = 9.83 PAKK101 pKa = 10.18 LRR103 pKa = 11.84 FFHH106 pKa = 7.18 RR107 pKa = 11.84 GPTHH111 pKa = 6.89 GDD113 pKa = 2.93 HH114 pKa = 6.98 FLNAHH119 pKa = 5.85 VEE121 pKa = 4.32 PKK123 pKa = 10.36 DD124 pKa = 3.63 VPRR127 pKa = 11.84 YY128 pKa = 7.32 PQEE131 pKa = 4.03 TIINRR136 pKa = 11.84 LADD139 pKa = 3.7 IPTQIAFMGDD149 pKa = 3.21 TLHH152 pKa = 6.77 FLSPSFLTALFAHH165 pKa = 6.62 SPRR168 pKa = 11.84 LQTLYY173 pKa = 9.97 ATLVLPPEE181 pKa = 4.68 PSTSRR186 pKa = 11.84 SLYY189 pKa = 7.71 PQIYY193 pKa = 7.8 TLTYY197 pKa = 9.98 SEE199 pKa = 5.37 DD200 pKa = 3.11 GFMYY204 pKa = 10.31 IPGGHH209 pKa = 6.81 AGASYY214 pKa = 9.36 FHH216 pKa = 7.49 KK217 pKa = 10.5 YY218 pKa = 9.84 DD219 pKa = 3.33 QLEE222 pKa = 4.31 WLTVGHH228 pKa = 6.85 LTAPGCPTVTAQRR241 pKa = 11.84 LEE243 pKa = 4.45 TKK245 pKa = 10.02 GANHH249 pKa = 7.04 LFIFQRR255 pKa = 11.84 GNYY258 pKa = 6.08 ITPEE262 pKa = 3.63 RR263 pKa = 11.84 RR264 pKa = 11.84 TFATNDD270 pKa = 3.18 QYY272 pKa = 11.39 VTVPHH277 pKa = 6.85 IFLPEE282 pKa = 3.97 NYY284 pKa = 9.63 NCRR287 pKa = 11.84 TPISKK292 pKa = 7.7 TTMMQMFFYY301 pKa = 10.35 IKK303 pKa = 10.0 SGKK306 pKa = 7.72 EE307 pKa = 3.75 AQRR310 pKa = 11.84 EE311 pKa = 4.1 GHH313 pKa = 6.92 LGQVQQLISTKK324 pKa = 9.37 EE325 pKa = 3.83 LQHH328 pKa = 5.56 YY329 pKa = 8.69 QPSEE333 pKa = 3.44 ITLLVNYY340 pKa = 9.83 FEE342 pKa = 4.74 FTATLDD348 pKa = 3.38 SHH350 pKa = 6.48 TCFEE354 pKa = 4.7 DD355 pKa = 3.9 VPPKK359 pKa = 10.19 LAPKK363 pKa = 7.39 TTPAPPLGISRR374 pKa = 11.84 ILQFFRR380 pKa = 11.84 GRR382 pKa = 11.84 PSFVKK387 pKa = 10.63 LIKK390 pKa = 10.78 ALDD393 pKa = 3.62 WQPITLEE400 pKa = 3.9 FPVRR404 pKa = 11.84 DD405 pKa = 2.97 VRR407 pKa = 11.84 TTVRR411 pKa = 11.84 GPPGFKK417 pKa = 10.69 NPFTQPQPVNPEE429 pKa = 3.94 DD430 pKa = 4.41 ADD432 pKa = 3.47 ISSRR436 pKa = 11.84 FQDD439 pKa = 4.02 FSRR442 pKa = 11.84 ATNSTPKK449 pKa = 8.97 PTVEE453 pKa = 4.06 AVLEE457 pKa = 4.26 ASAEE461 pKa = 4.38 SPDD464 pKa = 3.27 HH465 pKa = 5.65 HH466 pKa = 6.62 TQLKK470 pKa = 8.38 VLCSALSEE478 pKa = 4.33 GQLEE482 pKa = 4.71 AIHH485 pKa = 6.6 ALGHH489 pKa = 5.42 SRR491 pKa = 11.84 CGGPLLPEE499 pKa = 4.54 LTAKK503 pKa = 8.22 STPVEE508 pKa = 4.07 QCPDD512 pKa = 3.91 LPPCPTKK519 pKa = 10.81 SQASPASSNPSGPDD533 pKa = 3.37 AQPTAQNPEE542 pKa = 4.1 TLPWVAWLPKK552 pKa = 10.19 LNALGFEE559 pKa = 4.23 ALEE562 pKa = 4.26 VQHH565 pKa = 6.96 DD566 pKa = 4.02 PSTGEE571 pKa = 3.96 MIMPITDD578 pKa = 3.57 TQEE581 pKa = 3.91 LPRR584 pKa = 11.84 ATLPAHH590 pKa = 5.4 WQGHH594 pKa = 4.66 NLEE597 pKa = 5.54 RR598 pKa = 11.84 ILSTLRR604 pKa = 11.84 SLNRR608 pKa = 11.84 FPTPWSPDD616 pKa = 2.89 RR617 pKa = 11.84 TRR619 pKa = 11.84 ARR621 pKa = 11.84 AFTSDD626 pKa = 3.48 VKK628 pKa = 11.08 NSRR631 pKa = 11.84 TGKK634 pKa = 9.17 ALHH637 pKa = 6.88 RR638 pKa = 11.84 EE639 pKa = 4.03 SQTWKK644 pKa = 8.84 EE645 pKa = 4.27 TQTMLTEE652 pKa = 4.09 NTQTEE657 pKa = 4.15 LAISVIHH664 pKa = 6.17 GAGGSGKK671 pKa = 9.61 SQALQTLLRR680 pKa = 11.84 QDD682 pKa = 3.21 HH683 pKa = 6.32 SLPIEE688 pKa = 4.21 VVLPTNEE695 pKa = 5.49 LRR697 pKa = 11.84 LDD699 pKa = 3.6 WLKK702 pKa = 10.94 KK703 pKa = 10.15 LPHH706 pKa = 6.44 NPPEE710 pKa = 3.84 QFRR713 pKa = 11.84 TFEE716 pKa = 3.92 RR717 pKa = 11.84 AFVSSHH723 pKa = 5.64 SPVVIFDD730 pKa = 4.89 DD731 pKa = 4.56 YY732 pKa = 12.01 GKK734 pKa = 10.67 LPQGYY739 pKa = 9.41 LEE741 pKa = 5.01 AFALTHH747 pKa = 6.82 ANLEE751 pKa = 4.31 LVILTGDD758 pKa = 3.66 PRR760 pKa = 11.84 QSTHH764 pKa = 6.54 HH765 pKa = 6.38 EE766 pKa = 4.49 SNEE769 pKa = 3.55 EE770 pKa = 3.82 ALISKK775 pKa = 8.52 LPPATTIYY783 pKa = 10.61 SAFSRR788 pKa = 11.84 YY789 pKa = 9.46 YY790 pKa = 10.3 INATLRR796 pKa = 11.84 NSKK799 pKa = 10.16 FLANKK804 pKa = 9.92 LGVYY808 pKa = 9.43 SANSCPLNVTYY819 pKa = 10.46 GFQPLPGLHH828 pKa = 6.73 LLVPSLMKK836 pKa = 10.54 KK837 pKa = 10.14 AAFTDD842 pKa = 3.54 AGHH845 pKa = 6.48 KK846 pKa = 8.81 VSSYY850 pKa = 10.99 AGCQGITAPKK860 pKa = 9.87 VQILLDD866 pKa = 4.13 NDD868 pKa = 3.44 TTMCTKK874 pKa = 10.33 EE875 pKa = 3.99 VLYY878 pKa = 9.53 TALSRR883 pKa = 11.84 AVDD886 pKa = 3.91 SIHH889 pKa = 6.59 FVNTNAQSSAFWEE902 pKa = 4.27 KK903 pKa = 11.34 LEE905 pKa = 3.97 ATPYY909 pKa = 10.92 LKK911 pKa = 9.9 TFLSLVRR918 pKa = 11.84 EE919 pKa = 4.2 DD920 pKa = 5.59 KK921 pKa = 10.14 ITEE924 pKa = 4.1 FQAPADD930 pKa = 3.76 APKK933 pKa = 10.26 PVTPPVTHH941 pKa = 6.69 FPVEE945 pKa = 4.42 EE946 pKa = 4.17 PATTLDD952 pKa = 3.73 DD953 pKa = 4.28 FKK955 pKa = 11.74 EE956 pKa = 4.05 EE957 pKa = 4.5 LRR959 pKa = 11.84 DD960 pKa = 3.59 KK961 pKa = 10.66 FDD963 pKa = 4.55 RR964 pKa = 11.84 EE965 pKa = 4.02 IFTSDD970 pKa = 3.38 RR971 pKa = 11.84 GHH973 pKa = 6.15 TNCVQTDD980 pKa = 3.72 DD981 pKa = 4.24 PTTQLFSHH989 pKa = 5.86 QQAKK993 pKa = 10.86 DD994 pKa = 3.4 EE995 pKa = 4.3 ALLWEE1000 pKa = 4.93 MIRR1003 pKa = 11.84 ARR1005 pKa = 11.84 LKK1007 pKa = 10.62 ISTPEE1012 pKa = 3.72 ANIEE1016 pKa = 3.87 EE1017 pKa = 4.21 FRR1019 pKa = 11.84 AKK1021 pKa = 10.32 KK1022 pKa = 10.38 DD1023 pKa = 3.21 LGDD1026 pKa = 3.9 ILWVNYY1032 pKa = 8.42 HH1033 pKa = 6.64 RR1034 pKa = 11.84 AMGLPKK1040 pKa = 9.76 EE1041 pKa = 4.22 QPDD1044 pKa = 4.01 FSEE1047 pKa = 5.16 DD1048 pKa = 2.83 LWNRR1052 pKa = 11.84 CAEE1055 pKa = 4.26 EE1056 pKa = 4.0 VQNTYY1061 pKa = 10.35 LKK1063 pKa = 9.86 KK1064 pKa = 9.47 TMAQLKK1070 pKa = 9.72 GGEE1073 pKa = 4.2 RR1074 pKa = 11.84 RR1075 pKa = 11.84 QSPDD1079 pKa = 2.8 FHH1081 pKa = 6.51 EE1082 pKa = 4.28 HH1083 pKa = 6.21 QILIFLKK1090 pKa = 9.24 SQWVKK1095 pKa = 11.13 KK1096 pKa = 8.03 MEE1098 pKa = 4.12 KK1099 pKa = 10.31 LGAKK1103 pKa = 9.14 EE1104 pKa = 3.91 IKK1106 pKa = 9.72 PGQTIASFQQHH1117 pKa = 5.57 AVMLYY1122 pKa = 8.44 GTMARR1127 pKa = 11.84 YY1128 pKa = 7.8 MRR1130 pKa = 11.84 RR1131 pKa = 11.84 FRR1133 pKa = 11.84 DD1134 pKa = 3.29 ALSPNNIMINCEE1146 pKa = 3.97 KK1147 pKa = 10.25 TPQDD1151 pKa = 3.33 LSRR1154 pKa = 11.84 WVLNYY1159 pKa = 9.73 WDD1161 pKa = 4.51 FSKK1164 pKa = 10.74 PSYY1167 pKa = 11.38 ANDD1170 pKa = 3.11 FTAFDD1175 pKa = 3.83 QSQDD1179 pKa = 3.0 GAMLQFEE1186 pKa = 4.46 ILKK1189 pKa = 10.42 AKK1191 pKa = 10.13 YY1192 pKa = 9.62 FNLPEE1197 pKa = 4.07 WVIEE1201 pKa = 4.53 GYY1203 pKa = 11.05 LDD1205 pKa = 3.28 IKK1207 pKa = 10.77 LSPKK1211 pKa = 10.02 IFLGTLSIMRR1221 pKa = 11.84 LTGEE1225 pKa = 4.65 GPTFDD1230 pKa = 6.01 ANTEE1234 pKa = 4.01 CNIAYY1239 pKa = 6.91 THH1241 pKa = 5.92 TRR1243 pKa = 11.84 FDD1245 pKa = 3.64 IPEE1248 pKa = 4.23 GTAQIYY1254 pKa = 10.83 AGDD1257 pKa = 4.13 DD1258 pKa = 3.36 CAIAQVCPEE1267 pKa = 4.41 KK1268 pKa = 10.73 PSFALLKK1275 pKa = 10.76 NRR1277 pKa = 11.84 IALQAKK1283 pKa = 8.7 PAYY1286 pKa = 8.31 TPQEE1290 pKa = 3.93 RR1291 pKa = 11.84 GQWAEE1296 pKa = 3.63 FCGYY1300 pKa = 10.67 LITPKK1305 pKa = 10.87 GLIKK1309 pKa = 10.8 DD1310 pKa = 3.83 PLKK1313 pKa = 10.94 LHH1315 pKa = 7.1 ASLEE1319 pKa = 4.11 LAKK1322 pKa = 10.14 AQKK1325 pKa = 10.13 KK1326 pKa = 10.46 AGVRR1330 pKa = 11.84 DD1331 pKa = 4.36 AITNVVANYY1340 pKa = 10.89 SLDD1343 pKa = 3.57 AKK1345 pKa = 10.47 LAYY1348 pKa = 10.44 SLGDD1352 pKa = 3.86 DD1353 pKa = 4.29 LQDD1356 pKa = 4.47 LLSPHH1361 pKa = 6.0 QAHH1364 pKa = 6.71 LHH1366 pKa = 4.51 QVTVRR1371 pKa = 11.84 DD1372 pKa = 3.58 LVKK1375 pKa = 10.77 FGGSPFLNSDD1385 pKa = 3.09
Molecular weight: 155.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.162
IPC2_protein 6.084
IPC_protein 6.173
Toseland 6.59
ProMoST 6.547
Dawson 6.504
Bjellqvist 6.504
Wikipedia 6.532
Rodwell 6.504
Grimsley 6.766
Solomon 6.504
Lehninger 6.504
Nozaki 6.795
DTASelect 6.971
Thurlkill 7.0
EMBOSS 6.971
Sillero 6.898
Patrickios 4.253
IPC_peptide 6.532
IPC2_peptide 6.795
IPC2.peptide.svr19 6.789
Protein with the highest isoelectric point:
>sp|Q07520|TGB3_P1AMV Movement protein TGBp3 OS=Plantago asiatica mosaic potexvirus OX=28354 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.51 SGAHH5 pKa = 6.61 HH6 pKa = 6.84 LTPPTDD12 pKa = 3.28 YY13 pKa = 11.02 GKK15 pKa = 9.7 PVLAASIGISLALLVYY31 pKa = 8.77 TATRR35 pKa = 11.84 STLPHH40 pKa = 6.71 VGDD43 pKa = 3.98 NLHH46 pKa = 6.72 ALPHH50 pKa = 6.15 GGRR53 pKa = 11.84 YY54 pKa = 9.33 VDD56 pKa = 3.65 GTKK59 pKa = 10.35 SISYY63 pKa = 8.12 FSPSASKK70 pKa = 9.49 TRR72 pKa = 11.84 DD73 pKa = 3.36 PFPFAFLLILTLSGLILLLSRR94 pKa = 11.84 RR95 pKa = 11.84 RR96 pKa = 11.84 SNPHH100 pKa = 5.81 SCPSCGTPHH109 pKa = 7.11 AA110 pKa = 5.04
Molecular weight: 11.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.896
IPC2_protein 9.238
IPC_protein 9.458
Toseland 9.531
ProMoST 9.458
Dawson 9.867
Bjellqvist 9.692
Wikipedia 10.101
Rodwell 9.984
Grimsley 9.97
Solomon 9.955
Lehninger 9.911
Nozaki 9.706
DTASelect 9.648
Thurlkill 9.692
EMBOSS 9.97
Sillero 9.823
Patrickios 4.8
IPC_peptide 9.94
IPC2_peptide 8.829
IPC2.peptide.svr19 7.937
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2053
110
1385
410.6
45.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.206 ± 1.201
1.364 ± 0.188
4.384 ± 0.54
4.871 ± 1.122
4.676 ± 0.508
4.92 ± 0.381
3.653 ± 0.517
4.384 ± 0.29
4.433 ± 1.046
11.057 ± 0.815
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.315 ± 0.281
3.702 ± 0.214
8.475 ± 0.692
4.53 ± 0.772
4.579 ± 0.152
7.745 ± 1.278
8.475 ± 0.7
4.627 ± 0.262
0.974 ± 0.252
2.63 ± 0.504
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here