Clostridium sp. CAG:710

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5T2G3|R5T2G3_9CLOT 50S ribosomal protein L7/L12 OS=Clostridium sp. CAG:710 OX=1262833 GN=rplL PE=3 SV=1
MM1 pKa = 7.16TALEE5 pKa = 4.08LVKK8 pKa = 10.79KK9 pKa = 10.75KK10 pKa = 7.56MTNSGLDD17 pKa = 3.23SSEE20 pKa = 4.31TIGLSSTYY28 pKa = 10.13QISDD32 pKa = 4.24LDD34 pKa = 4.25CDD36 pKa = 4.87CVDD39 pKa = 4.75GDD41 pKa = 4.67CSTDD45 pKa = 3.3GYY47 pKa = 11.41EE48 pKa = 4.04SS49 pKa = 3.38

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5TMT5|R5TMT5_9CLOT Diguanylate cyclase and phosphoesterase OS=Clostridium sp. CAG:710 OX=1262833 GN=BN761_01139 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.39QPNNRR10 pKa = 11.84KK11 pKa = 9.04KK12 pKa = 10.09AKK14 pKa = 9.37KK15 pKa = 9.11HH16 pKa = 5.24GFFSRR21 pKa = 11.84IKK23 pKa = 10.57GNIINSRR30 pKa = 11.84RR31 pKa = 11.84KK32 pKa = 9.39KK33 pKa = 10.19GRR35 pKa = 11.84KK36 pKa = 9.14KK37 pKa = 10.64LVV39 pKa = 3.05

Molecular weight: 4.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1170

0

1170

345540

30

1554

295.3

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.422 ± 0.057

1.071 ± 0.028

6.189 ± 0.05

7.28 ± 0.079

4.278 ± 0.05

5.276 ± 0.065

1.224 ± 0.026

9.89 ± 0.089

10.144 ± 0.071

8.983 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.334 ± 0.033

7.264 ± 0.076

2.5 ± 0.037

1.985 ± 0.036

3.3 ± 0.047

6.527 ± 0.06

5.492 ± 0.063

6.228 ± 0.063

0.534 ± 0.02

5.077 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski