Amino acid dipepetide frequency for Peptostreptococcus sp. D1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.537AlaAla: 3.537 ± 0.102
0.709AlaCys: 0.709 ± 0.036
3.173AlaAsp: 3.173 ± 0.077
3.582AlaGlu: 3.582 ± 0.077
2.399AlaPhe: 2.399 ± 0.061
3.936AlaGly: 3.936 ± 0.082
0.777AlaHis: 0.777 ± 0.034
5.449AlaIle: 5.449 ± 0.094
4.638AlaLys: 4.638 ± 0.096
5.407AlaLeu: 5.407 ± 0.094
1.66AlaMet: 1.66 ± 0.054
2.817AlaAsn: 2.817 ± 0.065
1.427AlaPro: 1.427 ± 0.052
1.397AlaGln: 1.397 ± 0.053
2.351AlaArg: 2.351 ± 0.056
3.657AlaSer: 3.657 ± 0.08
2.889AlaThr: 2.889 ± 0.064
4.073AlaVal: 4.073 ± 0.088
0.347AlaTrp: 0.347 ± 0.024
2.052AlaTyr: 2.052 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.571CysAla: 0.571 ± 0.033
0.195CysCys: 0.195 ± 0.023
0.762CysAsp: 0.762 ± 0.034
0.763CysGlu: 0.763 ± 0.034
0.464CysPhe: 0.464 ± 0.031
1.041CysGly: 1.041 ± 0.05
0.219CysHis: 0.219 ± 0.018
0.96CysIle: 0.96 ± 0.039
0.825CysLys: 0.825 ± 0.041
0.774CysLeu: 0.774 ± 0.036
0.305CysMet: 0.305 ± 0.024
0.538CysAsn: 0.538 ± 0.034
0.439CysPro: 0.439 ± 0.033
0.188CysGln: 0.188 ± 0.017
0.436CysArg: 0.436 ± 0.026
0.804CysSer: 0.804 ± 0.035
0.508CysThr: 0.508 ± 0.03
0.756CysVal: 0.756 ± 0.037
0.08CysTrp: 0.08 ± 0.012
0.388CysTyr: 0.388 ± 0.028
0.0CysXaa: 0.0 ± 0.0
Asp
3.096AspAla: 3.096 ± 0.07
0.601AspCys: 0.601 ± 0.035
3.73AspAsp: 3.73 ± 0.081
5.374AspGlu: 5.374 ± 0.109
3.223AspPhe: 3.223 ± 0.08
4.05AspGly: 4.05 ± 0.103
0.533AspHis: 0.533 ± 0.026
6.61AspIle: 6.61 ± 0.107
5.532AspLys: 5.532 ± 0.089
4.931AspLeu: 4.931 ± 0.1
1.707AspMet: 1.707 ± 0.054
3.532AspAsn: 3.532 ± 0.078
1.324AspPro: 1.324 ± 0.043
0.867AspGln: 0.867 ± 0.035
2.575AspArg: 2.575 ± 0.062
4.038AspSer: 4.038 ± 0.086
2.743AspThr: 2.743 ± 0.07
4.02AspVal: 4.02 ± 0.071
0.388AspTrp: 0.388 ± 0.026
2.751AspTyr: 2.751 ± 0.067
0.0AspXaa: 0.0 ± 0.0
Glu
4.279GluAla: 4.279 ± 0.093
0.673GluCys: 0.673 ± 0.037
4.143GluAsp: 4.143 ± 0.097
5.775GluGlu: 5.775 ± 0.12
3.081GluPhe: 3.081 ± 0.073
3.7GluGly: 3.7 ± 0.081
0.925GluHis: 0.925 ± 0.039
7.558GluIle: 7.558 ± 0.112
8.29GluLys: 8.29 ± 0.122
6.956GluLeu: 6.956 ± 0.115
2.245GluMet: 2.245 ± 0.057
5.569GluAsn: 5.569 ± 0.094
1.316GluPro: 1.316 ± 0.049
1.756GluGln: 1.756 ± 0.06
2.939GluArg: 2.939 ± 0.064
4.032GluSer: 4.032 ± 0.078
2.608GluThr: 2.608 ± 0.067
4.463GluVal: 4.463 ± 0.088
0.497GluTrp: 0.497 ± 0.028
3.457GluTyr: 3.457 ± 0.076
0.0GluXaa: 0.0 ± 0.0
Phe
2.392PheAla: 2.392 ± 0.065
0.526PheCys: 0.526 ± 0.024
3.287PheAsp: 3.287 ± 0.067
3.307PheGlu: 3.307 ± 0.074
2.096PhePhe: 2.096 ± 0.067
3.027PheGly: 3.027 ± 0.066
0.499PheHis: 0.499 ± 0.03
4.035PheIle: 4.035 ± 0.091
3.236PheLys: 3.236 ± 0.074
3.6PheLeu: 3.6 ± 0.084
1.181PheMet: 1.181 ± 0.043
2.471PheAsn: 2.471 ± 0.067
1.185PhePro: 1.185 ± 0.046
0.807PheGln: 0.807 ± 0.038
1.46PheArg: 1.46 ± 0.051
3.416PheSer: 3.416 ± 0.076
2.178PheThr: 2.178 ± 0.066
3.146PheVal: 3.146 ± 0.084
0.314PheTrp: 0.314 ± 0.022
1.702PheTyr: 1.702 ± 0.056
0.0PheXaa: 0.0 ± 0.0
Gly
4.028GlyAla: 4.028 ± 0.098
0.835GlyCys: 0.835 ± 0.035
3.454GlyAsp: 3.454 ± 0.072
4.16GlyGlu: 4.16 ± 0.087
2.99GlyPhe: 2.99 ± 0.074
4.456GlyGly: 4.456 ± 0.109
1.005GlyHis: 1.005 ± 0.046
6.592GlyIle: 6.592 ± 0.104
6.0GlyLys: 6.0 ± 0.085
5.149GlyLeu: 5.149 ± 0.083
1.86GlyMet: 1.86 ± 0.056
3.373GlyAsn: 3.373 ± 0.073
1.127GlyPro: 1.127 ± 0.042
1.429GlyGln: 1.429 ± 0.05
2.515GlyArg: 2.515 ± 0.059
3.738GlySer: 3.738 ± 0.087
3.195GlyThr: 3.195 ± 0.079
4.851GlyVal: 4.851 ± 0.102
0.505GlyTrp: 0.505 ± 0.032
3.053GlyTyr: 3.053 ± 0.074
0.0GlyXaa: 0.0 ± 0.0
His
0.652HisAla: 0.652 ± 0.037
0.167HisCys: 0.167 ± 0.019
0.739HisAsp: 0.739 ± 0.031
0.919HisGlu: 0.919 ± 0.04
0.592HisPhe: 0.592 ± 0.035
0.886HisGly: 0.886 ± 0.042
0.258HisHis: 0.258 ± 0.02
1.236HisIle: 1.236 ± 0.039
1.002HisLys: 1.002 ± 0.041
1.146HisLeu: 1.146 ± 0.044
0.332HisMet: 0.332 ± 0.021
0.733HisAsn: 0.733 ± 0.032
0.535HisPro: 0.535 ± 0.031
0.334HisGln: 0.334 ± 0.023
0.61HisArg: 0.61 ± 0.029
0.968HisSer: 0.968 ± 0.042
0.69HisThr: 0.69 ± 0.034
0.684HisVal: 0.684 ± 0.033
0.084HisTrp: 0.084 ± 0.012
0.521HisTyr: 0.521 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.517IleAla: 5.517 ± 0.101
1.145IleCys: 1.145 ± 0.048
6.703IleAsp: 6.703 ± 0.108
7.506IleGlu: 7.506 ± 0.129
4.279IlePhe: 4.279 ± 0.09
5.978IleGly: 5.978 ± 0.112
1.184IleHis: 1.184 ± 0.045
7.992IleIle: 7.992 ± 0.141
8.077IleLys: 8.077 ± 0.12
8.231IleLeu: 8.231 ± 0.145
2.204IleMet: 2.204 ± 0.068
5.527IleAsn: 5.527 ± 0.114
3.044IlePro: 3.044 ± 0.063
1.985IleGln: 1.985 ± 0.047
3.362IleArg: 3.362 ± 0.061
7.528IleSer: 7.528 ± 0.12
4.414IleThr: 4.414 ± 0.086
6.738IleVal: 6.738 ± 0.11
0.473IleTrp: 0.473 ± 0.025
3.406IleTyr: 3.406 ± 0.086
0.0IleXaa: 0.0 ± 0.0
Lys
4.944LysAla: 4.944 ± 0.091
0.688LysCys: 0.688 ± 0.033
5.309LysAsp: 5.309 ± 0.101
7.28LysGlu: 7.28 ± 0.139
3.186LysPhe: 3.186 ± 0.066
4.654LysGly: 4.654 ± 0.094
1.176LysHis: 1.176 ± 0.047
8.419LysIle: 8.419 ± 0.124
9.172LysLys: 9.172 ± 0.12
7.66LysLeu: 7.66 ± 0.114
2.71LysMet: 2.71 ± 0.064
6.753LysAsn: 6.753 ± 0.111
2.114LysPro: 2.114 ± 0.07
2.166LysGln: 2.166 ± 0.062
3.263LysArg: 3.263 ± 0.073
5.709LysSer: 5.709 ± 0.111
4.07LysThr: 4.07 ± 0.082
5.254LysVal: 5.254 ± 0.095
0.572LysTrp: 0.572 ± 0.033
4.442LysTyr: 4.442 ± 0.088
0.0LysXaa: 0.0 ± 0.0
Leu
4.851LeuAla: 4.851 ± 0.091
0.986LeuCys: 0.986 ± 0.045
5.53LeuAsp: 5.53 ± 0.097
6.646LeuGlu: 6.646 ± 0.11
3.529LeuPhe: 3.529 ± 0.075
5.587LeuGly: 5.587 ± 0.101
0.971LeuHis: 0.971 ± 0.039
7.437LeuIle: 7.437 ± 0.129
7.716LeuLys: 7.716 ± 0.121
7.229LeuLeu: 7.229 ± 0.12
2.383LeuMet: 2.383 ± 0.06
4.998LeuAsn: 4.998 ± 0.094
2.617LeuPro: 2.617 ± 0.065
1.944LeuGln: 1.944 ± 0.062
3.221LeuArg: 3.221 ± 0.078
6.86LeuSer: 6.86 ± 0.129
4.077LeuThr: 4.077 ± 0.081
5.366LeuVal: 5.366 ± 0.101
0.458LeuTrp: 0.458 ± 0.027
2.984LeuTyr: 2.984 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
1.78MetAla: 1.78 ± 0.058
0.302MetCys: 0.302 ± 0.02
1.659MetAsp: 1.659 ± 0.049
1.848MetGlu: 1.848 ± 0.052
1.043MetPhe: 1.043 ± 0.04
1.755MetGly: 1.755 ± 0.054
0.284MetHis: 0.284 ± 0.02
2.387MetIle: 2.387 ± 0.057
2.637MetLys: 2.637 ± 0.056
2.291MetLeu: 2.291 ± 0.056
0.774MetMet: 0.774 ± 0.034
1.734MetAsn: 1.734 ± 0.05
0.846MetPro: 0.846 ± 0.037
0.632MetGln: 0.632 ± 0.032
1.092MetArg: 1.092 ± 0.046
1.964MetSer: 1.964 ± 0.056
1.33MetThr: 1.33 ± 0.044
1.675MetVal: 1.675 ± 0.047
0.152MetTrp: 0.152 ± 0.017
0.928MetTyr: 0.928 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
3.11AsnAla: 3.11 ± 0.075
0.625AsnCys: 0.625 ± 0.031
3.386AsnAsp: 3.386 ± 0.077
4.149AsnGlu: 4.149 ± 0.081
2.419AsnPhe: 2.419 ± 0.058
3.927AsnGly: 3.927 ± 0.086
0.844AsnHis: 0.844 ± 0.043
6.648AsnIle: 6.648 ± 0.12
5.52AsnLys: 5.52 ± 0.092
4.848AsnLeu: 4.848 ± 0.089
1.598AsnMet: 1.598 ± 0.053
3.936AsnAsn: 3.936 ± 0.093
2.117AsnPro: 2.117 ± 0.064
1.423AsnGln: 1.423 ± 0.052
2.438AsnArg: 2.438 ± 0.059
4.136AsnSer: 4.136 ± 0.082
3.066AsnThr: 3.066 ± 0.083
3.66AsnVal: 3.66 ± 0.074
0.389AsnTrp: 0.389 ± 0.026
2.419AsnTyr: 2.419 ± 0.062
0.0AsnXaa: 0.0 ± 0.0
Pro
1.445ProAla: 1.445 ± 0.057
0.305ProCys: 0.305 ± 0.022
1.623ProAsp: 1.623 ± 0.052
2.172ProGlu: 2.172 ± 0.064
1.256ProPhe: 1.256 ± 0.038
1.601ProGly: 1.601 ± 0.048
0.445ProHis: 0.445 ± 0.027
2.453ProIle: 2.453 ± 0.063
2.111ProLys: 2.111 ± 0.068
2.175ProLeu: 2.175 ± 0.064
0.681ProMet: 0.681 ± 0.034
1.537ProAsn: 1.537 ± 0.059
0.589ProPro: 0.589 ± 0.032
0.814ProGln: 0.814 ± 0.036
0.867ProArg: 0.867 ± 0.038
1.839ProSer: 1.839 ± 0.051
1.48ProThr: 1.48 ± 0.051
2.096ProVal: 2.096 ± 0.056
0.204ProTrp: 0.204 ± 0.017
1.083ProTyr: 1.083 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
1.492GlnAla: 1.492 ± 0.059
0.182GlnCys: 0.182 ± 0.016
1.104GlnAsp: 1.104 ± 0.036
1.532GlnGlu: 1.532 ± 0.057
0.888GlnPhe: 0.888 ± 0.043
1.442GlnGly: 1.442 ± 0.052
0.306GlnHis: 0.306 ± 0.023
2.193GlnIle: 2.193 ± 0.062
2.204GlnLys: 2.204 ± 0.066
1.971GlnLeu: 1.971 ± 0.051
0.732GlnMet: 0.732 ± 0.035
1.439GlnAsn: 1.439 ± 0.049
0.583GlnPro: 0.583 ± 0.033
0.651GlnGln: 0.651 ± 0.041
0.999GlnArg: 0.999 ± 0.04
1.384GlnSer: 1.384 ± 0.053
1.064GlnThr: 1.064 ± 0.048
1.394GlnVal: 1.394 ± 0.045
0.18GlnTrp: 0.18 ± 0.016
0.957GlnTyr: 0.957 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
2.234ArgAla: 2.234 ± 0.067
0.395ArgCys: 0.395 ± 0.024
2.196ArgAsp: 2.196 ± 0.057
3.14ArgGlu: 3.14 ± 0.073
1.711ArgPhe: 1.711 ± 0.046
2.266ArgGly: 2.266 ± 0.063
0.563ArgHis: 0.563 ± 0.031
3.576ArgIle: 3.576 ± 0.081
3.481ArgLys: 3.481 ± 0.066
3.434ArgLeu: 3.434 ± 0.071
1.119ArgMet: 1.119 ± 0.04
2.344ArgAsn: 2.344 ± 0.057
0.894ArgPro: 0.894 ± 0.038
1.056ArgGln: 1.056 ± 0.047
1.605ArgArg: 1.605 ± 0.058
1.906ArgSer: 1.906 ± 0.06
1.693ArgThr: 1.693 ± 0.055
2.679ArgVal: 2.679 ± 0.068
0.264ArgTrp: 0.264 ± 0.02
1.776ArgTyr: 1.776 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
3.274SerAla: 3.274 ± 0.078
0.724SerCys: 0.724 ± 0.037
4.103SerAsp: 4.103 ± 0.086
4.848SerGlu: 4.848 ± 0.079
3.352SerPhe: 3.352 ± 0.074
4.892SerGly: 4.892 ± 0.108
0.937SerHis: 0.937 ± 0.044
6.873SerIle: 6.873 ± 0.133
6.127SerLys: 6.127 ± 0.101
5.799SerLeu: 5.799 ± 0.101
1.735SerMet: 1.735 ± 0.054
4.208SerAsn: 4.208 ± 0.095
1.621SerPro: 1.621 ± 0.051
1.605SerGln: 1.605 ± 0.054
2.516SerArg: 2.516 ± 0.066
5.203SerSer: 5.203 ± 0.123
3.186SerThr: 3.186 ± 0.071
4.406SerVal: 4.406 ± 0.094
0.473SerTrp: 0.473 ± 0.025
2.749SerTyr: 2.749 ± 0.065
0.0SerXaa: 0.0 ± 0.0
Thr
2.814ThrAla: 2.814 ± 0.069
0.424ThrCys: 0.424 ± 0.026
2.841ThrAsp: 2.841 ± 0.073
2.933ThrGlu: 2.933 ± 0.073
1.95ThrPhe: 1.95 ± 0.061
3.684ThrGly: 3.684 ± 0.09
0.736ThrHis: 0.736 ± 0.035
4.441ThrIle: 4.441 ± 0.084
3.633ThrLys: 3.633 ± 0.084
3.984ThrLeu: 3.984 ± 0.089
1.091ThrMet: 1.091 ± 0.042
2.596ThrAsn: 2.596 ± 0.064
1.732ThrPro: 1.732 ± 0.058
1.145ThrGln: 1.145 ± 0.042
1.701ThrArg: 1.701 ± 0.044
3.281ThrSer: 3.281 ± 0.073
2.56ThrThr: 2.56 ± 0.072
3.43ThrVal: 3.43 ± 0.075
0.29ThrTrp: 0.29 ± 0.022
1.74ThrTyr: 1.74 ± 0.054
0.0ThrXaa: 0.0 ± 0.0
Val
3.965ValAla: 3.965 ± 0.084
0.829ValCys: 0.829 ± 0.045
4.596ValAsp: 4.596 ± 0.084
4.959ValGlu: 4.959 ± 0.099
3.164ValPhe: 3.164 ± 0.075
4.481ValGly: 4.481 ± 0.088
0.76ValHis: 0.76 ± 0.04
6.056ValIle: 6.056 ± 0.107
5.191ValLys: 5.191 ± 0.099
5.805ValLeu: 5.805 ± 0.107
1.62ValMet: 1.62 ± 0.049
3.655ValAsn: 3.655 ± 0.087
1.955ValPro: 1.955 ± 0.057
1.358ValGln: 1.358 ± 0.047
2.339ValArg: 2.339 ± 0.065
4.719ValSer: 4.719 ± 0.087
3.057ValThr: 3.057 ± 0.076
4.955ValVal: 4.955 ± 0.108
0.389ValTrp: 0.389 ± 0.026
2.619ValTyr: 2.619 ± 0.059
0.0ValXaa: 0.0 ± 0.0
Trp
0.326TrpAla: 0.326 ± 0.023
0.122TrpCys: 0.122 ± 0.014
0.437TrpAsp: 0.437 ± 0.025
0.454TrpGlu: 0.454 ± 0.027
0.279TrpPhe: 0.279 ± 0.021
0.412TrpGly: 0.412 ± 0.026
0.099TrpHis: 0.099 ± 0.012
0.572TrpIle: 0.572 ± 0.031
0.557TrpLys: 0.557 ± 0.031
0.518TrpLeu: 0.518 ± 0.03
0.197TrpMet: 0.197 ± 0.019
0.431TrpAsn: 0.431 ± 0.028
0.116TrpPro: 0.116 ± 0.013
0.209TrpGln: 0.209 ± 0.02
0.275TrpArg: 0.275 ± 0.019
0.428TrpSer: 0.428 ± 0.027
0.275TrpThr: 0.275 ± 0.023
0.347TrpVal: 0.347 ± 0.022
0.069TrpTrp: 0.069 ± 0.009
0.297TrpTyr: 0.297 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.971TyrAla: 1.971 ± 0.051
0.581TyrCys: 0.581 ± 0.029
2.848TyrAsp: 2.848 ± 0.067
2.916TyrGlu: 2.916 ± 0.075
1.965TyrPhe: 1.965 ± 0.058
2.537TyrGly: 2.537 ± 0.068
0.565TyrHis: 0.565 ± 0.028
3.802TyrIle: 3.802 ± 0.08
3.598TyrLys: 3.598 ± 0.084
3.481TyrLeu: 3.481 ± 0.07
0.978TyrMet: 0.978 ± 0.038
2.467TyrAsn: 2.467 ± 0.064
1.197TyrPro: 1.197 ± 0.05
0.933TyrGln: 0.933 ± 0.038
1.77TyrArg: 1.77 ± 0.058
3.023TyrSer: 3.023 ± 0.079
1.92TyrThr: 1.92 ± 0.053
2.479TyrVal: 2.479 ± 0.066
0.291TyrTrp: 0.291 ± 0.021
1.9TyrTyr: 1.9 ± 0.072
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2116 proteins (665628 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski