Freshwater phage uvFW-CGR-AMD-COM-C440

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0XU06|A0A1B0XU06_9CAUD Putative glycosyltransferase OS=Freshwater phage uvFW-CGR-AMD-COM-C440 OX=1838154 GN=uvFWCGRAMDCOMC440_09 PE=4 SV=1
MM1 pKa = 7.98PEE3 pKa = 4.19PRR5 pKa = 11.84LEE7 pKa = 4.93DD8 pKa = 4.95DD9 pKa = 2.89IAYY12 pKa = 10.14YY13 pKa = 10.17GYY15 pKa = 10.83EE16 pKa = 3.99MCDD19 pKa = 3.47DD20 pKa = 4.39CGDD23 pKa = 3.54RR24 pKa = 11.84HH25 pKa = 7.26DD26 pKa = 5.28PEE28 pKa = 4.18QSHH31 pKa = 6.79AGDD34 pKa = 4.25GEE36 pKa = 4.39PDD38 pKa = 3.75RR39 pKa = 11.84MWGDD43 pKa = 3.25EE44 pKa = 4.05DD45 pKa = 3.86

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0XU44|A0A1B0XU44_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMD-COM-C440 OX=1838154 GN=uvFWCGRAMDCOMC440_045 PE=4 SV=1
MM1 pKa = 7.99SSGQLKK7 pKa = 9.96RR8 pKa = 11.84HH9 pKa = 6.6DD10 pKa = 4.16GFNPVQIKK18 pKa = 10.57NGMIVRR24 pKa = 11.84LRR26 pKa = 11.84KK27 pKa = 10.26DD28 pKa = 3.36GTIKK32 pKa = 10.76AVLGKK37 pKa = 10.27YY38 pKa = 10.1GEE40 pKa = 4.21YY41 pKa = 10.75GKK43 pKa = 8.9QEE45 pKa = 3.96RR46 pKa = 11.84LKK48 pKa = 11.18ARR50 pKa = 3.65

Molecular weight: 5.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

10507

33

1946

184.3

20.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.555 ± 0.732

0.752 ± 0.199

5.73 ± 0.372

5.815 ± 0.41

3.16 ± 0.285

7.709 ± 0.775

1.38 ± 0.215

5.187 ± 0.257

5.882 ± 0.518

7.833 ± 0.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.185

4.873 ± 0.274

4.054 ± 0.236

4.226 ± 0.316

4.749 ± 0.509

6.957 ± 0.584

7.833 ± 0.759

5.891 ± 0.277

1.542 ± 0.152

3.493 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski