bacterium HR18

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2437 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5XL89|A0A2H5XL89_9BACT D-inositol 3-phosphate glycosyltransferase OS=bacterium HR18 OX=2035413 GN=mshA_2 PE=4 SV=1
MM1 pKa = 7.49SFFDD5 pKa = 5.6FGFDD9 pKa = 4.08DD10 pKa = 5.77ADD12 pKa = 3.93DD13 pKa = 4.13ALEE16 pKa = 3.93RR17 pKa = 11.84HH18 pKa = 5.25QLEE21 pKa = 4.44EE22 pKa = 3.98LVAAYY27 pKa = 7.81EE28 pKa = 4.61AYY30 pKa = 10.31GSSAYY35 pKa = 10.06FDD37 pKa = 3.74SDD39 pKa = 3.43ALEE42 pKa = 4.9EE43 pKa = 4.15IATYY47 pKa = 10.51YY48 pKa = 10.14YY49 pKa = 9.8EE50 pKa = 3.73QGRR53 pKa = 11.84FEE55 pKa = 4.19EE56 pKa = 4.33ALGVIDD62 pKa = 5.89RR63 pKa = 11.84LLKK66 pKa = 10.08LHH68 pKa = 6.43PTASDD73 pKa = 2.24AWMRR77 pKa = 11.84RR78 pKa = 11.84GILLSHH84 pKa = 7.21LGQHH88 pKa = 5.95EE89 pKa = 4.48EE90 pKa = 4.05ALQAYY95 pKa = 7.78QQALTLNPVDD105 pKa = 3.94TEE107 pKa = 4.35TLVNLGITLDD117 pKa = 3.44NLGRR121 pKa = 11.84FEE123 pKa = 4.42EE124 pKa = 4.29ALEE127 pKa = 4.47AYY129 pKa = 8.21EE130 pKa = 5.19QALQLDD136 pKa = 4.37PLNEE140 pKa = 3.46EE141 pKa = 4.09AYY143 pKa = 9.01YY144 pKa = 11.02NRR146 pKa = 11.84GITLEE151 pKa = 3.9RR152 pKa = 11.84MDD154 pKa = 4.8RR155 pKa = 11.84LAEE158 pKa = 4.6AIAAFEE164 pKa = 3.87QAAALNPEE172 pKa = 4.42HH173 pKa = 7.1PEE175 pKa = 3.29VWYY178 pKa = 10.14EE179 pKa = 4.61LGYY182 pKa = 11.03CYY184 pKa = 10.69DD185 pKa = 3.8RR186 pKa = 11.84LGDD189 pKa = 3.98DD190 pKa = 4.02EE191 pKa = 6.28RR192 pKa = 11.84SLACYY197 pKa = 9.77DD198 pKa = 3.21RR199 pKa = 11.84HH200 pKa = 6.48LALDD204 pKa = 4.87PYY206 pKa = 11.26SADD209 pKa = 2.4AWYY212 pKa = 10.81NRR214 pKa = 11.84GIVLNRR220 pKa = 11.84MGRR223 pKa = 11.84YY224 pKa = 8.17EE225 pKa = 4.12EE226 pKa = 5.36AVASYY231 pKa = 10.93DD232 pKa = 3.55FAIAIQDD239 pKa = 3.91DD240 pKa = 4.09FASAYY245 pKa = 9.18YY246 pKa = 10.68NRR248 pKa = 11.84GNALTNLGDD257 pKa = 3.52LRR259 pKa = 11.84GAIEE263 pKa = 4.31SYY265 pKa = 10.85EE266 pKa = 3.99KK267 pKa = 10.84VLEE270 pKa = 4.18IEE272 pKa = 4.79GGDD275 pKa = 3.27PATYY279 pKa = 10.87YY280 pKa = 10.95NIALAYY286 pKa = 9.86EE287 pKa = 4.21EE288 pKa = 4.1LQEE291 pKa = 4.15YY292 pKa = 6.92EE293 pKa = 3.76TAIAYY298 pKa = 7.53FQQALEE304 pKa = 4.17EE305 pKa = 4.52DD306 pKa = 3.69PTYY309 pKa = 11.47AEE311 pKa = 3.47AWYY314 pKa = 10.45GLGCCYY320 pKa = 10.48DD321 pKa = 3.19ALEE324 pKa = 4.38RR325 pKa = 11.84FEE327 pKa = 4.92EE328 pKa = 4.53AVACLEE334 pKa = 4.07RR335 pKa = 11.84AVALRR340 pKa = 11.84PEE342 pKa = 4.52ASEE345 pKa = 3.84FWYY348 pKa = 10.51AKK350 pKa = 10.45ADD352 pKa = 3.52CEE354 pKa = 4.34YY355 pKa = 10.22NARR358 pKa = 11.84RR359 pKa = 11.84VEE361 pKa = 4.15AALHH365 pKa = 5.74SYY367 pKa = 9.9HH368 pKa = 6.78RR369 pKa = 11.84VVSLDD374 pKa = 3.24PTNRR378 pKa = 11.84EE379 pKa = 3.2AWLDD383 pKa = 3.57YY384 pKa = 11.4AEE386 pKa = 4.36TLFEE390 pKa = 4.41VGLTEE395 pKa = 4.22EE396 pKa = 4.15ALEE399 pKa = 4.69AYY401 pKa = 9.85RR402 pKa = 11.84QALSLNPDD410 pKa = 2.42ARR412 pKa = 11.84AYY414 pKa = 9.57IRR416 pKa = 11.84QARR419 pKa = 11.84ALLALGRR426 pKa = 11.84SEE428 pKa = 4.36EE429 pKa = 4.6GIRR432 pKa = 11.84SLKK435 pKa = 9.03MALRR439 pKa = 11.84LDD441 pKa = 4.14PSAKK445 pKa = 10.29DD446 pKa = 3.56EE447 pKa = 4.26LPEE450 pKa = 4.32FYY452 pKa = 10.1RR453 pKa = 11.84DD454 pKa = 3.08ASIRR458 pKa = 11.84RR459 pKa = 11.84QLGLDD464 pKa = 3.54GG465 pKa = 4.79

Molecular weight: 52.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5XMM0|A0A2H5XMM0_9BACT 30S ribosomal protein S19 OS=bacterium HR18 OX=2035413 GN=rpsS PE=3 SV=1
MM1 pKa = 7.37LVEE4 pKa = 4.05KK5 pKa = 10.51VRR7 pKa = 11.84RR8 pKa = 11.84FIEE11 pKa = 4.16LNGLLRR17 pKa = 11.84PGEE20 pKa = 4.04RR21 pKa = 11.84LLVGVSGGVDD31 pKa = 3.2SVVLLEE37 pKa = 4.09VLRR40 pKa = 11.84QLGYY44 pKa = 10.25SLIVAHH50 pKa = 6.34VNYY53 pKa = 9.96KK54 pKa = 10.59LRR56 pKa = 11.84GADD59 pKa = 3.27SDD61 pKa = 3.76ADD63 pKa = 3.84EE64 pKa = 4.86AFVRR68 pKa = 11.84ALCRR72 pKa = 11.84QYY74 pKa = 10.84RR75 pKa = 11.84IPIRR79 pKa = 11.84VARR82 pKa = 11.84LNLKK86 pKa = 10.25AKK88 pKa = 10.05VRR90 pKa = 11.84GGSIQAAARR99 pKa = 11.84SVRR102 pKa = 11.84YY103 pKa = 10.01AFFARR108 pKa = 11.84VAQRR112 pKa = 11.84EE113 pKa = 4.58GIEE116 pKa = 4.03AVAVGHH122 pKa = 6.28HH123 pKa = 6.23QDD125 pKa = 3.92DD126 pKa = 3.81QAEE129 pKa = 4.26TLLLNLLRR137 pKa = 11.84SSGLEE142 pKa = 3.89GLTGMAPVRR151 pKa = 11.84PFEE154 pKa = 4.3VGQKK158 pKa = 10.04LRR160 pKa = 11.84LVRR163 pKa = 11.84PLLSVTRR170 pKa = 11.84AEE172 pKa = 4.02IEE174 pKa = 3.62SWARR178 pKa = 11.84AKK180 pKa = 10.56GLSWRR185 pKa = 11.84EE186 pKa = 3.87DD187 pKa = 3.46VSNTSLNYY195 pKa = 9.58RR196 pKa = 11.84RR197 pKa = 11.84VLVRR201 pKa = 11.84QVVLPLLRR209 pKa = 11.84QHH211 pKa = 6.95FGPNVAEE218 pKa = 4.21RR219 pKa = 11.84LAHH222 pKa = 5.07TAEE225 pKa = 4.35LLRR228 pKa = 11.84AYY230 pKa = 7.74WQSTFAQVLMQHH242 pKa = 6.42WLSASEE248 pKa = 3.94SDD250 pKa = 4.25AVGGYY255 pKa = 9.53LRR257 pKa = 11.84QEE259 pKa = 4.49ALAGMPRR266 pKa = 11.84VWQQRR271 pKa = 11.84LILEE275 pKa = 4.23ALKK278 pKa = 10.35RR279 pKa = 11.84WLPGAPRR286 pKa = 11.84HH287 pKa = 6.5RR288 pKa = 11.84RR289 pKa = 11.84LAWQVLRR296 pKa = 11.84LLEE299 pKa = 4.29AQPGRR304 pKa = 11.84RR305 pKa = 11.84LKK307 pKa = 10.87LPQGTIWRR315 pKa = 11.84DD316 pKa = 3.08RR317 pKa = 11.84KK318 pKa = 9.67GLRR321 pKa = 11.84FRR323 pKa = 11.84RR324 pKa = 11.84CAPAAPISEE333 pKa = 4.34EE334 pKa = 4.1GALEE338 pKa = 4.1PGRR341 pKa = 11.84PLVLEE346 pKa = 4.34GGVFEE351 pKa = 6.05AVLLSQKK358 pKa = 9.21PACLNAGTPQVVYY371 pKa = 10.83VDD373 pKa = 4.05ADD375 pKa = 4.05RR376 pKa = 11.84LQWPLWVRR384 pKa = 11.84RR385 pKa = 11.84WRR387 pKa = 11.84PGDD390 pKa = 3.34RR391 pKa = 11.84LQPLGMQGHH400 pKa = 6.45KK401 pKa = 10.14KK402 pKa = 10.69VSDD405 pKa = 3.9LLTDD409 pKa = 3.64LRR411 pKa = 11.84VPVDD415 pKa = 3.99RR416 pKa = 11.84RR417 pKa = 11.84AHH419 pKa = 5.97SYY421 pKa = 8.57VVCQGEE427 pKa = 4.29EE428 pKa = 4.21IVWVAGYY435 pKa = 10.55RR436 pKa = 11.84LAEE439 pKa = 4.23PFRR442 pKa = 11.84VRR444 pKa = 11.84PEE446 pKa = 4.19TVHH449 pKa = 6.86IAQLQFTPIEE459 pKa = 4.28DD460 pKa = 3.33

Molecular weight: 52.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2437

0

2437

885271

29

2540

363.3

40.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.668 ± 0.061

0.756 ± 0.016

4.542 ± 0.034

6.5 ± 0.05

3.799 ± 0.031

7.507 ± 0.048

2.375 ± 0.024

4.481 ± 0.034

2.335 ± 0.032

12.087 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.854 ± 0.018

2.424 ± 0.034

5.751 ± 0.04

4.565 ± 0.038

8.144 ± 0.04

4.376 ± 0.032

5.321 ± 0.034

7.589 ± 0.038

1.658 ± 0.024

3.267 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski