Herbaspirillum sp. K2R10-39

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Herbaspirillum; unclassified Herbaspirillum

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4168 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A418X3Y8|A0A418X3Y8_9BURK N-acetyl-gamma-glutamyl-phosphate reductase OS=Herbaspirillum sp. K2R10-39 OX=2320862 GN=argC PE=3 SV=1
MM1 pKa = 7.28NAVTDD6 pKa = 4.48LEE8 pKa = 4.53MPSPIVFTDD17 pKa = 3.61SAAAKK22 pKa = 8.97VAQLIEE28 pKa = 4.39EE29 pKa = 4.51EE30 pKa = 4.63GNPDD34 pKa = 2.85LKK36 pKa = 11.13LRR38 pKa = 11.84VFVQGGGCSGFQYY51 pKa = 10.94GFTFDD56 pKa = 5.2EE57 pKa = 4.78IVNDD61 pKa = 5.33DD62 pKa = 4.44DD63 pKa = 3.75TTMDD67 pKa = 3.4KK68 pKa = 11.38NGVQLLIDD76 pKa = 3.82AMSYY80 pKa = 10.32QYY82 pKa = 11.5LVGAEE87 pKa = 4.01IDD89 pKa = 3.89YY90 pKa = 11.28KK91 pKa = 11.39DD92 pKa = 4.47DD93 pKa = 4.08LEE95 pKa = 4.56GAQFVIKK102 pKa = 10.5NPNATTTCGCGSSFSVV118 pKa = 3.54

Molecular weight: 12.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A418WWP0|A0A418WWP0_9BURK Exo-alpha-sialidase OS=Herbaspirillum sp. K2R10-39 OX=2320862 GN=D3870_20570 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.19RR14 pKa = 11.84THH16 pKa = 5.61GFRR19 pKa = 11.84VRR21 pKa = 11.84MATRR25 pKa = 11.84SGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4168

0

4168

1333771

29

5427

320.0

34.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.753 ± 0.058

0.942 ± 0.013

5.36 ± 0.028

5.495 ± 0.041

3.728 ± 0.027

7.866 ± 0.053

2.261 ± 0.022

5.388 ± 0.027

4.113 ± 0.038

10.102 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.021

3.266 ± 0.037

4.768 ± 0.029

3.87 ± 0.025

6.349 ± 0.036

5.747 ± 0.033

5.335 ± 0.038

7.232 ± 0.034

1.279 ± 0.016

2.456 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski