Salmonella phage Seszw_1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411BF29|A0A411BF29_9CAUD Uncharacterized protein OS=Salmonella phage Seszw_1 OX=2479482 GN=Seszw_24 PE=4 SV=1
MM1 pKa = 7.66CIDD4 pKa = 3.41IAILGFNMNMLEE16 pKa = 4.86LVDD19 pKa = 6.25AYY21 pKa = 11.13LQDD24 pKa = 3.68AGLYY28 pKa = 10.23DD29 pKa = 3.78GWTSQLQFWNDD40 pKa = 3.18TGDD43 pKa = 3.49GNEE46 pKa = 3.97QFIVLQSNGGTQVMDD61 pKa = 4.77GIGGDD66 pKa = 4.17FYY68 pKa = 11.56FSLYY72 pKa = 10.79VVGKK76 pKa = 9.37QGQYY80 pKa = 11.08NLQDD84 pKa = 2.97VDD86 pKa = 3.83AKK88 pKa = 10.87ANEE91 pKa = 3.92IIEE94 pKa = 4.39YY95 pKa = 10.2IKK97 pKa = 9.03THH99 pKa = 6.97PIDD102 pKa = 3.39SCVNYY107 pKa = 9.96IQLQAPLGRR116 pKa = 11.84PMLTEE121 pKa = 4.25EE122 pKa = 4.25KK123 pKa = 10.49RR124 pKa = 11.84PVHH127 pKa = 6.06EE128 pKa = 4.22LLLRR132 pKa = 11.84VVKK135 pKa = 10.78

Molecular weight: 15.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411BFB0|A0A411BFB0_9CAUD Uncharacterized protein OS=Salmonella phage Seszw_1 OX=2479482 GN=Seszw_33 PE=4 SV=1
MM1 pKa = 7.37SKK3 pKa = 10.58CSTIHH8 pKa = 5.29QLVMADD14 pKa = 2.9IRR16 pKa = 11.84EE17 pKa = 4.26SNARR21 pKa = 11.84NKK23 pKa = 10.24KK24 pKa = 9.88SRR26 pKa = 11.84NQKK29 pKa = 8.53NWYY32 pKa = 9.45GGIGFLKK39 pKa = 10.94AMFKK43 pKa = 10.57NSKK46 pKa = 8.53KK47 pKa = 9.74HH48 pKa = 4.13YY49 pKa = 9.3RR50 pKa = 11.84RR51 pKa = 11.84DD52 pKa = 3.54RR53 pKa = 11.84ILRR56 pKa = 11.84RR57 pKa = 11.84LCDD60 pKa = 4.02LNMKK64 pKa = 10.33KK65 pKa = 10.1LVKK68 pKa = 10.45EE69 pKa = 3.85MTKK72 pKa = 10.83

Molecular weight: 8.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

13274

35

969

184.4

20.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.784 ± 0.605

1.499 ± 0.181

6.155 ± 0.262

6.569 ± 0.344

3.307 ± 0.146

7.104 ± 0.31

1.838 ± 0.217

6.69 ± 0.206

6.599 ± 0.366

7.21 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.149 ± 0.199

4.708 ± 0.258

3.39 ± 0.239

4.015 ± 0.334

5.243 ± 0.27

6.667 ± 0.41

5.454 ± 0.389

6.381 ± 0.268

1.499 ± 0.112

3.737 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski