Arthrobacter phage Sporto

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6THE0|A0A5J6THE0_9CAUD Uncharacterized protein OS=Arthrobacter phage Sporto OX=2599835 GN=11 PE=4 SV=1
MM1 pKa = 6.88SQQVALTTVDD11 pKa = 3.03NPYY14 pKa = 11.36DD15 pKa = 3.44VFTQFNEE22 pKa = 3.47WNAWDD27 pKa = 4.33LSMGYY32 pKa = 7.69GTCAYY37 pKa = 9.46LARR40 pKa = 11.84VVRR43 pKa = 11.84TSDD46 pKa = 3.37EE47 pKa = 4.3LSVTDD52 pKa = 3.77QDD54 pKa = 4.16LALEE58 pKa = 4.06YY59 pKa = 10.85GIDD62 pKa = 3.56EE63 pKa = 5.2IIRR66 pKa = 11.84EE67 pKa = 4.31DD68 pKa = 3.88VLALYY73 pKa = 10.21KK74 pKa = 10.56KK75 pKa = 10.41VVADD79 pKa = 3.94HH80 pKa = 7.41DD81 pKa = 4.3PVKK84 pKa = 11.0VDD86 pKa = 3.51TTSEE90 pKa = 3.84

Molecular weight: 10.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6THE9|A0A5J6THE9_9CAUD Uncharacterized protein OS=Arthrobacter phage Sporto OX=2599835 GN=21 PE=4 SV=1
MM1 pKa = 7.78AEE3 pKa = 3.8VDD5 pKa = 4.18NFLAHH10 pKa = 6.27YY11 pKa = 9.52GVTGMKK17 pKa = 9.15WGVRR21 pKa = 11.84KK22 pKa = 10.29GGVKK26 pKa = 10.23SRR28 pKa = 11.84IQGAASDD35 pKa = 3.95SVEE38 pKa = 3.69RR39 pKa = 11.84RR40 pKa = 11.84LVRR43 pKa = 11.84DD44 pKa = 3.24RR45 pKa = 11.84AIATGTATTRR55 pKa = 11.84DD56 pKa = 3.66YY57 pKa = 11.26RR58 pKa = 11.84RR59 pKa = 11.84NIAKK63 pKa = 10.08GGTFGVVTGTRR74 pKa = 11.84QKK76 pKa = 10.9SAAKK80 pKa = 8.74RR81 pKa = 11.84TKK83 pKa = 10.41KK84 pKa = 10.79LEE86 pKa = 4.07ALKK89 pKa = 11.01DD90 pKa = 3.55RR91 pKa = 11.84VDD93 pKa = 3.54NGKK96 pKa = 7.97TSTWDD101 pKa = 3.26KK102 pKa = 11.35VGIAMNTHH110 pKa = 6.75PGDD113 pKa = 3.6LVVSRR118 pKa = 11.84RR119 pKa = 11.84DD120 pKa = 3.24KK121 pKa = 10.93RR122 pKa = 11.84ALPDD126 pKa = 3.43SPAAKK131 pKa = 10.5VNTGKK136 pKa = 10.29QKK138 pKa = 10.78ALKK141 pKa = 10.05IMAGAAGAVAISVASNKK158 pKa = 9.37QNQAMAADD166 pKa = 4.37IVRR169 pKa = 11.84MGMDD173 pKa = 3.03AAKK176 pKa = 10.13RR177 pKa = 11.84AKK179 pKa = 9.61TNRR182 pKa = 11.84TNIRR186 pKa = 11.84NDD188 pKa = 3.14YY189 pKa = 9.94SDD191 pKa = 3.61SHH193 pKa = 6.46GLPTGPTIRR202 pKa = 11.84LHH204 pKa = 5.97QNPTTGNWVV213 pKa = 3.04

Molecular weight: 22.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

17161

40

1516

204.3

22.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.933 ± 0.526

0.635 ± 0.111

6.101 ± 0.297

6.328 ± 0.368

3.724 ± 0.209

7.82 ± 0.533

1.812 ± 0.202

5.973 ± 0.273

6.445 ± 0.421

7.861 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.96 ± 0.284

4.44 ± 0.181

4.079 ± 0.304

3.374 ± 0.167

4.965 ± 0.292

5.984 ± 0.213

6.433 ± 0.358

7.097 ± 0.256

1.573 ± 0.162

3.461 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski