Trichogramma brassicae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita; Parasitoida; Chalcidoidea; Trichogrammatidae; Trichogramma

Average proteome isoelectric point is 7.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16797 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H5J4X4|A0A6H5J4X4_9HYME Uncharacterized protein (Fragment) OS=Trichogramma brassicae OX=86971 GN=TBRA_LOCUS15205 PE=4 SV=1
MM1 pKa = 7.61ASADD5 pKa = 3.62SSEE8 pKa = 4.51ASCATDD14 pKa = 3.42PNFAVICSFIEE25 pKa = 4.06QFGKK29 pKa = 9.97HH30 pKa = 6.16CGLEE34 pKa = 4.29TPDD37 pKa = 3.71IASLQDD43 pKa = 3.61WIDD46 pKa = 3.43NTQDD50 pKa = 3.73EE51 pKa = 4.9IVKK54 pKa = 8.7TWLLL58 pKa = 3.58

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H5IPH3|A0A6H5IPH3_9HYME Uncharacterized protein OS=Trichogramma brassicae OX=86971 GN=TBRA_LOCUS11193 PE=4 SV=1
MM1 pKa = 7.34SSARR5 pKa = 11.84RR6 pKa = 11.84IVEE9 pKa = 3.59RR10 pKa = 11.84RR11 pKa = 11.84PRR13 pKa = 11.84TRR15 pKa = 11.84AAAAAAASWLLLLLLLASWSGSCQAAAAAATTSSASTSSNIISSSSISNGGGGPTTASTSATIATTSSKK84 pKa = 10.61KK85 pKa = 10.22VATSSSSSSISSSSSSSSSSSSSSLNPVPAVTIQSRR121 pKa = 11.84RR122 pKa = 11.84KK123 pKa = 7.88RR124 pKa = 11.84RR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84GRR130 pKa = 11.84RR131 pKa = 11.84RR132 pKa = 11.84RR133 pKa = 11.84KK134 pKa = 8.55LLRR137 pKa = 11.84RR138 pKa = 11.84RR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 11.84LLHH144 pKa = 6.44RR145 pKa = 11.84LL146 pKa = 3.3

Molecular weight: 15.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16797

0

16797

8277567

49

8693

492.8

55.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.733 ± 0.02

2.276 ± 0.017

5.257 ± 0.017

6.001 ± 0.021

3.433 ± 0.013

5.453 ± 0.017

2.9 ± 0.011

5.026 ± 0.015

5.558 ± 0.023

8.863 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.97 ± 0.008

4.239 ± 0.015

5.129 ± 0.023

4.357 ± 0.023

7.925 ± 0.031

8.279 ± 0.026

5.695 ± 0.016

5.818 ± 0.011

1.127 ± 0.006

2.962 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski