Pteropus associated gemycircularvirus 7
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTQ5|A0A140CTQ5_9VIRU RepA OS=Pteropus associated gemycircularvirus 7 OX=1985401 PE=3 SV=1
MM1 pKa = 7.72 APFILKK7 pKa = 9.68 NVRR10 pKa = 11.84 RR11 pKa = 11.84 ALITYY16 pKa = 7.03 SQCGNLDD23 pKa = 3.41 PFEE26 pKa = 4.42 VSNHH30 pKa = 5.21 FSNLGAEE37 pKa = 4.53 CIVGRR42 pKa = 11.84 EE43 pKa = 3.85 RR44 pKa = 11.84 HH45 pKa = 5.69 SDD47 pKa = 3.32 GGIHH51 pKa = 6.34 LHH53 pKa = 5.85 VFVDD57 pKa = 4.6 FGRR60 pKa = 11.84 QFSSRR65 pKa = 11.84 KK66 pKa = 7.81 TDD68 pKa = 3.11 VFDD71 pKa = 5.05 VGGCHH76 pKa = 6.86 PNIAKK81 pKa = 10.4 CGRR84 pKa = 11.84 TPWKK88 pKa = 10.45 AYY90 pKa = 10.52 DD91 pKa = 3.69 YY92 pKa = 10.6 AIKK95 pKa = 10.92 DD96 pKa = 3.52 GDD98 pKa = 4.01 VVAGGLEE105 pKa = 4.14 RR106 pKa = 11.84 PDD108 pKa = 3.99 EE109 pKa = 4.22 EE110 pKa = 5.45 SGDD113 pKa = 3.82 GSGSVDD119 pKa = 3.91 STWHH123 pKa = 6.36 SILAAEE129 pKa = 4.52 TPEE132 pKa = 4.32 EE133 pKa = 4.32 FWSLCHH139 pKa = 7.16 DD140 pKa = 4.78 LAPRR144 pKa = 11.84 DD145 pKa = 3.72 LARR148 pKa = 11.84 SFPSLQKK155 pKa = 10.22 YY156 pKa = 9.88 CDD158 pKa = 2.84 WRR160 pKa = 11.84 YY161 pKa = 10.12 RR162 pKa = 11.84 PVTRR166 pKa = 11.84 PYY168 pKa = 9.3 STPAGYY174 pKa = 10.65 DD175 pKa = 3.22 FDD177 pKa = 4.43 TSCAPEE183 pKa = 4.25 LDD185 pKa = 3.52 EE186 pKa = 6.07 WLLQANLGLRR196 pKa = 11.84 EE197 pKa = 4.05 LGLRR201 pKa = 3.92
Molecular weight: 22.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.12
IPC2_protein 5.181
IPC_protein 5.143
Toseland 5.219
ProMoST 5.359
Dawson 5.232
Bjellqvist 5.321
Wikipedia 5.156
Rodwell 5.156
Grimsley 5.194
Solomon 5.232
Lehninger 5.194
Nozaki 5.397
DTASelect 5.588
Thurlkill 5.296
EMBOSS 5.296
Sillero 5.461
Patrickios 3.757
IPC_peptide 5.245
IPC2_peptide 5.474
IPC2.peptide.svr19 5.376
Protein with the highest isoelectric point:
>tr|A0A140CTQ4|A0A140CTQ4_9VIRU Replication-associated protein OS=Pteropus associated gemycircularvirus 7 OX=1985401 PE=3 SV=1
MM1 pKa = 7.49 PRR3 pKa = 11.84 YY4 pKa = 9.86 ANRR7 pKa = 11.84 RR8 pKa = 11.84 ISKK11 pKa = 10.11 KK12 pKa = 6.42 YY13 pKa = 8.64 TKK15 pKa = 9.42 TKK17 pKa = 8.96 RR18 pKa = 11.84 KK19 pKa = 8.87 PYY21 pKa = 9.61 RR22 pKa = 11.84 RR23 pKa = 11.84 PARR26 pKa = 11.84 PSKK29 pKa = 10.66 KK30 pKa = 10.22 YY31 pKa = 10.44 GGKK34 pKa = 9.96 SSLKK38 pKa = 10.53 KK39 pKa = 9.91 MLNLVSTKK47 pKa = 10.34 KK48 pKa = 10.15 RR49 pKa = 11.84 DD50 pKa = 3.49 TMLTYY55 pKa = 11.15 SNTSASKK62 pKa = 9.82 PAGDD66 pKa = 4.05 TNYY69 pKa = 10.23 GAHH72 pKa = 7.52 DD73 pKa = 3.57 ATLIGGQSYY82 pKa = 10.16 IFPWVATARR91 pKa = 11.84 PAITTDD97 pKa = 3.07 NNAGFPIDD105 pKa = 3.71 QACRR109 pKa = 11.84 TASTCYY115 pKa = 9.54 MRR117 pKa = 11.84 GLKK120 pKa = 9.81 EE121 pKa = 4.55 RR122 pKa = 11.84 ITMQTNTGSAWQWRR136 pKa = 11.84 RR137 pKa = 11.84 ICFTMKK143 pKa = 10.4 GDD145 pKa = 3.72 QLYY148 pKa = 8.1 STDD151 pKa = 3.35 GSNLRR156 pKa = 11.84 WSLMANEE163 pKa = 4.41 VGGVTGMVRR172 pKa = 11.84 VLNSVSGSNAGSTLVDD188 pKa = 4.88 LIFRR192 pKa = 11.84 GKK194 pKa = 10.47 FGQDD198 pKa = 2.75 WNNVMSAPLDD208 pKa = 3.59 QNLITVKK215 pKa = 10.28 YY216 pKa = 9.67 DD217 pKa = 3.06 KK218 pKa = 10.58 TRR220 pKa = 11.84 SIQSGNDD227 pKa = 3.22 DD228 pKa = 4.51 GILRR232 pKa = 11.84 TYY234 pKa = 10.68 SRR236 pKa = 11.84 WHH238 pKa = 6.38 PMAKK242 pKa = 9.96 NLVYY246 pKa = 10.74 GDD248 pKa = 4.43 DD249 pKa = 3.84 EE250 pKa = 5.0 NGGFEE255 pKa = 3.95 DD256 pKa = 3.46 TRR258 pKa = 11.84 RR259 pKa = 11.84 YY260 pKa = 9.63 SAGGKK265 pKa = 10.08 AGMGDD270 pKa = 4.07 YY271 pKa = 10.92 YY272 pKa = 11.33 VIDD275 pKa = 5.13 IINSALGSAPTDD287 pKa = 3.49 QLILGINSILYY298 pKa = 7.96 WHH300 pKa = 7.02 EE301 pKa = 3.84 KK302 pKa = 9.39
Molecular weight: 33.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.167
IPC2_protein 9.385
IPC_protein 9.414
Toseland 9.882
ProMoST 9.692
Dawson 10.145
Bjellqvist 9.838
Wikipedia 10.335
Rodwell 10.467
Grimsley 10.233
Solomon 10.16
Lehninger 10.116
Nozaki 9.897
DTASelect 9.838
Thurlkill 9.97
EMBOSS 10.306
Sillero 10.058
Patrickios 7.571
IPC_peptide 10.16
IPC2_peptide 8.536
IPC2.peptide.svr19 8.411
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
820
201
317
273.3
30.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.951 ± 0.006
2.317 ± 0.587
7.439 ± 0.51
4.146 ± 1.073
4.146 ± 0.93
9.146 ± 0.196
2.561 ± 0.667
4.39 ± 0.534
5.0 ± 0.906
7.683 ± 0.523
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.829 ± 0.782
4.024 ± 0.969
5.122 ± 0.63
2.439 ± 0.237
6.585 ± 0.574
8.293 ± 0.148
5.732 ± 1.091
5.122 ± 0.501
2.683 ± 0.219
4.39 ± 0.417
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here