Flavonifractor sp. An100

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Flavonifractor; unclassified Flavonifractor

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2837 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4WDK9|A0A1Y4WDK9_9FIRM Sodium/proline symporter OS=Flavonifractor sp. An100 OX=1965538 GN=B5E43_05940 PE=3 SV=1
MM1 pKa = 7.46KK2 pKa = 10.19KK3 pKa = 10.07YY4 pKa = 10.72LAMLLAGALSVSLLAGCGGDD24 pKa = 3.42SGSSASSTGSAGSAGSASSTGSDD47 pKa = 2.91SSAADD52 pKa = 3.23GTTFKK57 pKa = 11.16LGGTGPLTGGASIYY71 pKa = 10.99GLAAQRR77 pKa = 11.84GAQIAVDD84 pKa = 5.0EE85 pKa = 4.29IAEE88 pKa = 4.32AGGDD92 pKa = 3.35IQFTLRR98 pKa = 11.84YY99 pKa = 9.15EE100 pKa = 4.69DD101 pKa = 4.68DD102 pKa = 3.44VHH104 pKa = 7.95DD105 pKa = 4.74AEE107 pKa = 5.17KK108 pKa = 10.8AVNAYY113 pKa = 7.7NTLKK117 pKa = 10.12EE118 pKa = 3.69WGMQISLGSVTSKK131 pKa = 10.47PGEE134 pKa = 3.98ATSVLNYY141 pKa = 9.51EE142 pKa = 4.34DD143 pKa = 5.67RR144 pKa = 11.84IFALTPSASSPAVIEE159 pKa = 4.45SKK161 pKa = 11.32DD162 pKa = 3.66NVFQMCFSDD171 pKa = 4.13PNQGLASAQYY181 pKa = 10.3ISEE184 pKa = 4.2QNLGEE189 pKa = 4.14KK190 pKa = 10.54VFIIWKK196 pKa = 9.7NDD198 pKa = 3.04DD199 pKa = 3.72VYY201 pKa = 11.32STGIKK206 pKa = 10.23DD207 pKa = 3.59QFVTEE212 pKa = 4.57AEE214 pKa = 4.07ALNLNVVGDD223 pKa = 3.96ATFTTDD229 pKa = 2.55SQTDD233 pKa = 3.6FTVQLTQAQQAGADD247 pKa = 3.97LLFLPIYY254 pKa = 10.09YY255 pKa = 10.24DD256 pKa = 3.53AASLILSQANAMGYY270 pKa = 10.4DD271 pKa = 3.59PAIFGVDD278 pKa = 3.3GMDD281 pKa = 5.41GILTVDD287 pKa = 3.77GFDD290 pKa = 3.39PALAEE295 pKa = 4.09GVMLLTPFNADD306 pKa = 3.28AQDD309 pKa = 3.58EE310 pKa = 4.36ATQAFVQKK318 pKa = 10.36YY319 pKa = 4.86QAQYY323 pKa = 11.52GEE325 pKa = 4.48VPNQFAADD333 pKa = 4.02AYY335 pKa = 10.34DD336 pKa = 3.58CVYY339 pKa = 11.15ALKK342 pKa = 10.43QALEE346 pKa = 4.38TAGCTPDD353 pKa = 3.74MSAEE357 pKa = 4.47DD358 pKa = 3.53ICTKK362 pKa = 10.42LIEE365 pKa = 4.37VFPTITFEE373 pKa = 4.31GLTGDD378 pKa = 3.95GAGITWDD385 pKa = 3.34STGAVSKK392 pKa = 10.55SPKK395 pKa = 10.53GMVIQNGAYY404 pKa = 10.28VGMDD408 pKa = 3.03

Molecular weight: 42.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4WAV0|A0A1Y4WAV0_9FIRM Acetolactate synthase OS=Flavonifractor sp. An100 OX=1965538 GN=B5E43_06015 PE=3 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.8QPKK8 pKa = 9.6KK9 pKa = 7.95RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.69EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 10.07RR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.04GRR39 pKa = 11.84ARR41 pKa = 11.84LTHH44 pKa = 6.28

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2837

0

2837

881498

35

3337

310.7

34.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.093 ± 0.057

1.596 ± 0.018

5.427 ± 0.036

6.84 ± 0.053

3.661 ± 0.034

7.951 ± 0.041

1.85 ± 0.024

5.503 ± 0.039

4.68 ± 0.047

10.244 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.021

3.221 ± 0.025

4.356 ± 0.031

4.128 ± 0.03

5.653 ± 0.049

5.741 ± 0.037

5.553 ± 0.039

7.139 ± 0.04

1.159 ± 0.019

3.46 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski