Arcobacter anaerophilus
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2919 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q0XZV5|A0A4Q0XZV5_9PROT Uncharacterized protein OS=Arcobacter anaerophilus OX=877500 GN=CRV06_06135 PE=4 SV=1
MM1 pKa = 7.54 EE2 pKa = 5.48 SIIYY6 pKa = 8.55 EE7 pKa = 3.91 LRR9 pKa = 11.84 ANNIKK14 pKa = 10.7 EE15 pKa = 4.04 EE16 pKa = 4.79 DD17 pKa = 3.44 IQSIMEE23 pKa = 4.1 KK24 pKa = 9.54 VKK26 pKa = 10.88 NRR28 pKa = 11.84 ISEE31 pKa = 4.14 EE32 pKa = 4.08 NIDD35 pKa = 5.12 IEE37 pKa = 4.38 LQKK40 pKa = 10.97 LGYY43 pKa = 8.86 PKK45 pKa = 10.81 LFTVDD50 pKa = 3.13 YY51 pKa = 10.74 DD52 pKa = 5.05 DD53 pKa = 5.11 YY54 pKa = 12.03 DD55 pKa = 4.78 LYY57 pKa = 11.28 DD58 pKa = 4.23 YY59 pKa = 11.66 DD60 pKa = 5.78 DD61 pKa = 5.64 GYY63 pKa = 11.58 DD64 pKa = 3.62 DD65 pKa = 3.76 EE66 pKa = 5.7 QYY68 pKa = 11.82
Molecular weight: 8.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.884
IPC_protein 3.834
Toseland 3.63
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.139
Thurlkill 3.681
EMBOSS 3.757
Sillero 3.948
Patrickios 1.888
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.847
Protein with the highest isoelectric point:
>tr|A0A4Q0XYP4|A0A4Q0XYP4_9PROT Uncharacterized protein OS=Arcobacter anaerophilus OX=877500 GN=CRV06_10755 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.31 RR14 pKa = 11.84 THH16 pKa = 6.08 GFRR19 pKa = 11.84 IRR21 pKa = 11.84 MKK23 pKa = 9.04 TKK25 pKa = 10.16 NGRR28 pKa = 11.84 KK29 pKa = 8.88 VIKK32 pKa = 10.14 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.79 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2919
0
2919
924466
37
2724
316.7
36.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.836 ± 0.04
0.909 ± 0.017
5.629 ± 0.038
7.399 ± 0.044
5.285 ± 0.042
5.369 ± 0.045
1.554 ± 0.018
9.23 ± 0.047
9.95 ± 0.067
9.761 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.272 ± 0.02
6.11 ± 0.042
2.756 ± 0.026
2.795 ± 0.023
2.996 ± 0.026
6.603 ± 0.036
4.951 ± 0.04
5.794 ± 0.033
0.732 ± 0.014
4.07 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here