Streptococcus satellite phage Javan256

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZMJ1|A0A4D5ZMJ1_9VIRU RelB/StbD replicon stabilization protein OS=Streptococcus satellite phage Javan256 OX=2558593 GN=JavanS256_0016 PE=4 SV=1
MM1 pKa = 6.53TTEE4 pKa = 4.46QILDD8 pKa = 3.79SYY10 pKa = 11.58EE11 pKa = 4.31SLTSDD16 pKa = 2.9MMKK19 pKa = 10.43AVSALNIVRR28 pKa = 11.84RR29 pKa = 11.84WLEE32 pKa = 3.73NVKK35 pKa = 10.61KK36 pKa = 10.47SDD38 pKa = 2.86EE39 pKa = 4.28GYY41 pKa = 10.7YY42 pKa = 10.85DD43 pKa = 5.25FIAFKK48 pKa = 10.1EE49 pKa = 4.36AYY51 pKa = 10.13ADD53 pKa = 4.02IINLAMNDD61 pKa = 3.86LQRR64 pKa = 11.84LASEE68 pKa = 4.33HH69 pKa = 5.95YY70 pKa = 10.41KK71 pKa = 10.65FIEE74 pKa = 4.1RR75 pKa = 11.84AEE77 pKa = 3.95KK78 pKa = 11.0DD79 pKa = 3.51EE80 pKa = 4.43FSEE83 pKa = 5.22AEE85 pKa = 3.87

Molecular weight: 9.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZH88|A0A4D5ZH88_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan256 OX=2558593 GN=JavanS256_0011 PE=4 SV=1
MM1 pKa = 7.55NIKK4 pKa = 10.55DD5 pKa = 3.92KK6 pKa = 10.89IKK8 pKa = 11.03KK9 pKa = 9.53NGQKK13 pKa = 10.04VYY15 pKa = 10.21YY16 pKa = 9.67ASVYY20 pKa = 10.7LGVDD24 pKa = 2.95QLTGKK29 pKa = 10.14KK30 pKa = 10.31ARR32 pKa = 11.84TTVTATTKK40 pKa = 10.63KK41 pKa = 10.27GVKK44 pKa = 8.67TKK46 pKa = 10.83ARR48 pKa = 11.84DD49 pKa = 3.59VINAFAANGYY59 pKa = 5.7TVKK62 pKa = 10.68DD63 pKa = 3.48KK64 pKa = 9.74PTITTYY70 pKa = 11.08KK71 pKa = 10.18EE72 pKa = 4.03LVKK75 pKa = 10.72VWWDD79 pKa = 3.31SYY81 pKa = 11.64KK82 pKa = 10.2NTVKK86 pKa = 10.73PNTRR90 pKa = 11.84QSMEE94 pKa = 3.66GLVRR98 pKa = 11.84VHH100 pKa = 6.91LLPVFGDD107 pKa = 3.68YY108 pKa = 10.69KK109 pKa = 10.62LSKK112 pKa = 9.42LTTPIIQHH120 pKa = 5.63QVNKK124 pKa = 9.59WADD127 pKa = 3.39KK128 pKa = 11.42ANTEE132 pKa = 4.1QKK134 pKa = 10.97GAFTNYY140 pKa = 10.64SLLHH144 pKa = 5.79NMNKK148 pKa = 10.25RR149 pKa = 11.84ILKK152 pKa = 10.11YY153 pKa = 10.19GVSLQVIPHH162 pKa = 6.2NPANDD167 pKa = 3.43VIVPRR172 pKa = 11.84KK173 pKa = 8.24RR174 pKa = 11.84QKK176 pKa = 10.72EE177 pKa = 4.15KK178 pKa = 8.73TTVKK182 pKa = 10.72YY183 pKa = 10.72LGNKK187 pKa = 7.16EE188 pKa = 4.11LKK190 pKa = 10.43QFLDD194 pKa = 3.68YY195 pKa = 11.33LDD197 pKa = 4.79TLDD200 pKa = 3.68QSNYY204 pKa = 9.49EE205 pKa = 3.97NLFDD209 pKa = 3.81VVLYY213 pKa = 8.25KK214 pKa = 10.5TLLATGCRR222 pKa = 11.84ISEE225 pKa = 4.1ALALEE230 pKa = 4.39WSDD233 pKa = 3.55IDD235 pKa = 4.56LDD237 pKa = 4.06NGIISINKK245 pKa = 7.21TLNRR249 pKa = 11.84YY250 pKa = 7.65QEE252 pKa = 4.1INSPKK257 pKa = 10.13SSAGYY262 pKa = 10.09RR263 pKa = 11.84DD264 pKa = 3.43IPIDD268 pKa = 3.67NATLLMLKK276 pKa = 9.77QYY278 pKa = 11.18KK279 pKa = 9.65NRR281 pKa = 11.84QQVQSWQLGRR291 pKa = 11.84SEE293 pKa = 4.28TVVFSVFTEE302 pKa = 4.94KK303 pKa = 10.81YY304 pKa = 10.17AYY306 pKa = 10.08SCNLRR311 pKa = 11.84RR312 pKa = 11.84RR313 pKa = 11.84LDD315 pKa = 3.44KK316 pKa = 10.72HH317 pKa = 6.28FKK319 pKa = 9.95AAGVTNVSFHH329 pKa = 6.03GFRR332 pKa = 11.84HH333 pKa = 4.73THH335 pKa = 4.12TTMMLYY341 pKa = 10.52AQASPKK347 pKa = 10.17DD348 pKa = 3.49VQYY351 pKa = 11.49RR352 pKa = 11.84LGHH355 pKa = 6.09SSLMMTEE362 pKa = 3.5NVYY365 pKa = 9.01WHH367 pKa = 6.72TNQEE371 pKa = 4.08NAKK374 pKa = 9.53KK375 pKa = 10.29AVSNYY380 pKa = 6.06EE381 pKa = 3.8TAINSLL387 pKa = 3.69

Molecular weight: 44.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

3301

62

483

183.4

21.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.119 ± 0.413

0.636 ± 0.213

5.332 ± 0.254

7.907 ± 0.626

3.666 ± 0.413

4.605 ± 0.297

2.181 ± 0.304

6.513 ± 0.716

9.391 ± 0.432

9.603 ± 0.365

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.193

5.029 ± 0.472

2.939 ± 0.388

4.968 ± 0.261

4.756 ± 0.318

5.635 ± 0.341

6.453 ± 0.581

5.726 ± 0.415

1.181 ± 0.145

4.938 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski