Wenling hoplichthys paramyxovirus
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1GNJ8|A0A2P1GNJ8_9MONO Fusion glycoprotein F0 OS=Wenling hoplichthys paramyxovirus OX=2116453 PE=3 SV=1
MM1 pKa = 7.43 TMGTLVFMLLVLLAGEE17 pKa = 4.58 CKK19 pKa = 10.66 GGDD22 pKa = 3.91 EE23 pKa = 4.51 SCLYY27 pKa = 10.19 PPALIQQEE35 pKa = 4.07 GKK37 pKa = 9.88 IFYY40 pKa = 10.21 NGVPGTTRR48 pKa = 11.84 LVSTDD53 pKa = 3.51 RR54 pKa = 11.84 VICPSTGCNIISDD67 pKa = 4.05 GRR69 pKa = 11.84 LSASADD75 pKa = 3.4 AYY77 pKa = 10.96 GSMDD81 pKa = 3.11 MHH83 pKa = 6.83 KK84 pKa = 10.24 RR85 pKa = 11.84 DD86 pKa = 3.36 YY87 pKa = 11.19 EE88 pKa = 4.22 FYY90 pKa = 10.2 KK91 pKa = 10.93 DD92 pKa = 4.18 KK93 pKa = 10.71 IVSRR97 pKa = 11.84 AGGALVANILRR108 pKa = 11.84 TRR110 pKa = 11.84 ANTALGFYY118 pKa = 9.23 EE119 pKa = 5.71 SCAEE123 pKa = 3.68 WKK125 pKa = 10.08 IFYY128 pKa = 9.85 EE129 pKa = 4.57 GVVFSPYY136 pKa = 9.12 MRR138 pKa = 11.84 QACTGTNGEE147 pKa = 4.09 WSRR150 pKa = 11.84 MKK152 pKa = 10.39 EE153 pKa = 3.58 QFYY156 pKa = 10.12 TKK158 pKa = 9.62 FYY160 pKa = 11.06 EE161 pKa = 3.95 MATRR165 pKa = 11.84 FQCFKK170 pKa = 10.36 FVRR173 pKa = 11.84 VPYY176 pKa = 9.97 IRR178 pKa = 11.84 PAEE181 pKa = 4.22 STQEE185 pKa = 3.95 VAPGCGAGGTINGVEE200 pKa = 4.64 YY201 pKa = 9.42 ITDD204 pKa = 3.65 PRR206 pKa = 11.84 NGTIITSVVTDD217 pKa = 3.67 LKK219 pKa = 10.46 ITGEE223 pKa = 4.18 YY224 pKa = 10.75 LMPFCYY230 pKa = 9.33 FVYY233 pKa = 10.23 RR234 pKa = 11.84 SRR236 pKa = 11.84 DD237 pKa = 2.4 IWLAEE242 pKa = 3.52 NRR244 pKa = 11.84 GYY246 pKa = 11.21 DD247 pKa = 3.26 SDD249 pKa = 4.66 PFLKK253 pKa = 10.58 VYY255 pKa = 10.72 NEE257 pKa = 3.92 QGEE260 pKa = 4.63 TLFDD264 pKa = 3.75 WNQLTDD270 pKa = 4.05 GYY272 pKa = 11.1 GEE274 pKa = 4.07 PKK276 pKa = 9.61 AEE278 pKa = 4.44 KK279 pKa = 9.49 MIKK282 pKa = 9.81 CSFIIVEE289 pKa = 4.4 KK290 pKa = 9.62 TSKK293 pKa = 8.97 ITIQSWSASQFITRR307 pKa = 11.84 AAYY310 pKa = 9.78 YY311 pKa = 10.2 FNEE314 pKa = 4.76 TIPRR318 pKa = 11.84 RR319 pKa = 11.84 PIQVIEE325 pKa = 4.48 SKK327 pKa = 9.51 WAQSRR332 pKa = 11.84 MSDD335 pKa = 4.27 PITQEE340 pKa = 3.86 SACRR344 pKa = 11.84 RR345 pKa = 11.84 LGDD348 pKa = 3.28 YY349 pKa = 10.72 CIYY352 pKa = 10.55 FRR354 pKa = 11.84 IGTYY358 pKa = 10.44 LGIFDD363 pKa = 5.86 DD364 pKa = 4.74 EE365 pKa = 5.43 LGDD368 pKa = 3.75 LQVRR372 pKa = 11.84 DD373 pKa = 3.81 ILSGQKK379 pKa = 10.41 GGGPNFCGGRR389 pKa = 11.84 SYY391 pKa = 11.23 PGGCPASCNPSPFCTDD407 pKa = 4.03 YY408 pKa = 11.37 ISMGGATAHH417 pKa = 6.2 MASPYY422 pKa = 10.05 SSYY425 pKa = 11.2 RR426 pKa = 11.84 SFGSLYY432 pKa = 10.41 EE433 pKa = 4.12 KK434 pKa = 8.64 WTLTYY439 pKa = 10.24 PGGEE443 pKa = 4.21 SVSTLLHH450 pKa = 5.46 QLSLSMASEE459 pKa = 4.31 WNTGSITPEE468 pKa = 4.09 GEE470 pKa = 3.93 PTLSLLGMDD479 pKa = 4.08 SEE481 pKa = 4.37 ISNFEE486 pKa = 3.93 TLGYY490 pKa = 9.18 TKK492 pKa = 10.65 DD493 pKa = 3.51 PEE495 pKa = 4.29 TTRR498 pKa = 11.84 WCVTLSCEE506 pKa = 3.97 NCTNLFDD513 pKa = 4.04 TRR515 pKa = 11.84 TGRR518 pKa = 11.84 PRR520 pKa = 11.84 IAPFVASRR528 pKa = 11.84 IKK530 pKa = 11.03 AEE532 pKa = 3.95 GLAEE536 pKa = 4.07 IEE538 pKa = 5.0 MRR540 pKa = 11.84 DD541 pKa = 3.49 YY542 pKa = 11.14 WNDD545 pKa = 2.71 VSLIIALCLLLALIIWLIVTSVLLTDD571 pKa = 3.29 VRR573 pKa = 11.84 RR574 pKa = 11.84 TKK576 pKa = 10.29 KK577 pKa = 10.03 HH578 pKa = 5.51 PRR580 pKa = 11.84 SSIDD584 pKa = 3.18 PSMEE588 pKa = 3.99 GYY590 pKa = 10.3 VADD593 pKa = 4.49 LL594 pKa = 3.98
Molecular weight: 66.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.085
IPC2_protein 5.359
IPC_protein 5.296
Toseland 5.207
ProMoST 5.423
Dawson 5.283
Bjellqvist 5.41
Wikipedia 5.143
Rodwell 5.207
Grimsley 5.13
Solomon 5.283
Lehninger 5.245
Nozaki 5.397
DTASelect 5.55
Thurlkill 5.245
EMBOSS 5.194
Sillero 5.486
Patrickios 3.274
IPC_peptide 5.296
IPC2_peptide 5.474
IPC2.peptide.svr19 5.488
Protein with the highest isoelectric point:
>tr|A0A2P1GN21|A0A2P1GN21_9MONO Nucleocapsid OS=Wenling hoplichthys paramyxovirus OX=2116453 PE=3 SV=1
MM1 pKa = 7.72 AARR4 pKa = 11.84 EE5 pKa = 4.17 LSRR8 pKa = 11.84 AAYY11 pKa = 10.02 DD12 pKa = 3.36 HH13 pKa = 6.02 EE14 pKa = 4.64 TGIKK18 pKa = 10.41 GPTDD22 pKa = 3.16 GTKK25 pKa = 10.32 CYY27 pKa = 7.48 TTYY30 pKa = 11.06 NPWGVGYY37 pKa = 7.67 DD38 pKa = 3.4 TSQISVFAIGDD49 pKa = 3.45 IGKK52 pKa = 10.11 DD53 pKa = 3.18 KK54 pKa = 10.64 ILKK57 pKa = 9.41 NLRR60 pKa = 11.84 INLGLLTEE68 pKa = 4.47 GMTRR72 pKa = 11.84 EE73 pKa = 3.9 QAADD77 pKa = 4.95 LITGTSFSAVKK88 pKa = 10.7 VMGVNEE94 pKa = 4.79 TIRR97 pKa = 11.84 IKK99 pKa = 10.78 PNSNMEE105 pKa = 4.39 ALRR108 pKa = 11.84 KK109 pKa = 8.51 ITGLKK114 pKa = 9.72 MGVQTALGPVCCPKK128 pKa = 10.76 AYY130 pKa = 10.11 VFPVGKK136 pKa = 9.9 QLDD139 pKa = 3.61 VRR141 pKa = 11.84 FVHH144 pKa = 7.01 VIFTVLPYY152 pKa = 10.56 KK153 pKa = 10.8 AGTHH157 pKa = 5.75 KK158 pKa = 10.94 LGGGILNLQKK168 pKa = 9.84 TKK170 pKa = 10.4 IVGGISVNVQTTGKK184 pKa = 8.52 MGKK187 pKa = 8.82 IDD189 pKa = 4.6 LPLTIPFMEE198 pKa = 4.32 WKK200 pKa = 10.21 KK201 pKa = 8.74 STEE204 pKa = 4.2 GVFEE208 pKa = 4.39 LMGMVMGASLKK219 pKa = 10.16 VALGGVNGLSIHH231 pKa = 5.27 VTAIGQIHH239 pKa = 7.44 KK240 pKa = 9.81 DD241 pKa = 3.48 LKK243 pKa = 8.61 GTGMNKK249 pKa = 9.84 NMMLCPVFMYY259 pKa = 10.53 PDD261 pKa = 3.31 IVFSNCLKK269 pKa = 8.61 MWKK272 pKa = 8.07 VTSAKK277 pKa = 10.26 LILQIQPRR285 pKa = 11.84 VFRR288 pKa = 11.84 PIEE291 pKa = 4.05 EE292 pKa = 4.37 PLVSEE297 pKa = 5.14 RR298 pKa = 11.84 DD299 pKa = 3.75 LLTKK303 pKa = 10.26 ALKK306 pKa = 10.74 SGGEE310 pKa = 4.06 EE311 pKa = 3.72 EE312 pKa = 4.87 DD313 pKa = 3.5
Molecular weight: 34.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.828
IPC2_protein 8.639
IPC_protein 8.507
Toseland 9.428
ProMoST 9.092
Dawson 9.648
Bjellqvist 9.355
Wikipedia 9.765
Rodwell 10.101
Grimsley 9.677
Solomon 9.677
Lehninger 9.663
Nozaki 9.589
DTASelect 9.297
Thurlkill 9.516
EMBOSS 9.838
Sillero 9.619
Patrickios 5.626
IPC_peptide 9.677
IPC2_peptide 7.922
IPC2.peptide.svr19 7.923
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
4722
140
2055
524.7
58.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.612 ± 0.474
2.308 ± 0.333
5.04 ± 0.22
7.264 ± 0.622
3.346 ± 0.293
7.116 ± 0.46
1.588 ± 0.274
7.793 ± 0.444
6.777 ± 0.779
9.022 ± 0.464
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.283 ± 0.277
4.299 ± 0.401
3.685 ± 0.252
2.944 ± 0.239
5.548 ± 0.446
8.492 ± 0.563
6.501 ± 0.428
5.294 ± 0.437
1.207 ± 0.203
2.88 ± 0.42
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here