Capybara microvirus Cap1_SP_121
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7V5|A0A4P8W7V5_9VIRU Nonstructural protein OS=Capybara microvirus Cap1_SP_121 OX=2584812 PE=4 SV=1
MM1 pKa = 7.58 IFGSRR6 pKa = 11.84 TNLPKK11 pKa = 10.0 TVYY14 pKa = 10.49 APAGEE19 pKa = 4.58 KK20 pKa = 9.94 EE21 pKa = 4.25 VNTYY25 pKa = 10.29 ILEE28 pKa = 4.17 TDD30 pKa = 3.21 KK31 pKa = 11.69 NGNTFLTVDD40 pKa = 3.74 GTEE43 pKa = 4.73 DD44 pKa = 3.53 IYY46 pKa = 11.73 AKK48 pKa = 9.29 TQADD52 pKa = 4.06 LEE54 pKa = 4.35 DD55 pKa = 3.96 SKK57 pKa = 11.32 IEE59 pKa = 4.07 NIIARR64 pKa = 11.84 VQYY67 pKa = 11.06 GDD69 pKa = 3.31 ISALSARR76 pKa = 11.84 QGIYY80 pKa = 10.84 GDD82 pKa = 3.3 IAEE85 pKa = 4.91 MPKK88 pKa = 10.0 SWAEE92 pKa = 3.88 AQTKK96 pKa = 8.25 MMAMEE101 pKa = 4.35 QIFNKK106 pKa = 10.24 LPLEE110 pKa = 3.98 MRR112 pKa = 11.84 EE113 pKa = 4.3 SYY115 pKa = 10.51 GFNYY119 pKa = 10.04 KK120 pKa = 10.59 AFIADD125 pKa = 3.57 YY126 pKa = 10.61 GSEE129 pKa = 3.95 KK130 pKa = 10.32 FMKK133 pKa = 10.52 AIGITKK139 pKa = 9.64 PEE141 pKa = 4.37 PDD143 pKa = 3.17 AAAPEE148 pKa = 4.19 QEE150 pKa = 4.0 KK151 pKa = 11.41 GEE153 pKa = 4.29 TKK155 pKa = 10.25 EE156 pKa = 3.85
Molecular weight: 17.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.743
IPC2_protein 4.736
IPC_protein 4.609
Toseland 4.457
ProMoST 4.635
Dawson 4.533
Bjellqvist 4.724
Wikipedia 4.38
Rodwell 4.444
Grimsley 4.368
Solomon 4.533
Lehninger 4.482
Nozaki 4.647
DTASelect 4.749
Thurlkill 4.444
EMBOSS 4.393
Sillero 4.711
Patrickios 4.037
IPC_peptide 4.546
IPC2_peptide 4.698
IPC2.peptide.svr19 4.69
Protein with the highest isoelectric point:
>tr|A0A4P8W598|A0A4P8W598_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_121 OX=2584812 PE=4 SV=1
MM1 pKa = 7.4 NKK3 pKa = 9.5 EE4 pKa = 3.7 QTTTISIEE12 pKa = 3.9 LTQAEE17 pKa = 4.45 IKK19 pKa = 9.33 TIKK22 pKa = 9.9 RR23 pKa = 11.84 AKK25 pKa = 9.81 KK26 pKa = 10.15 LNADD30 pKa = 3.43 IYY32 pKa = 10.36 GKK34 pKa = 10.54 RR35 pKa = 11.84 IQFEE39 pKa = 4.18 NYY41 pKa = 8.91 IKK43 pKa = 10.55 GRR45 pKa = 11.84 LMADD49 pKa = 2.58 SRR51 pKa = 11.84 MILSMWYY58 pKa = 9.62 EE59 pKa = 4.09 SRR61 pKa = 11.84 AEE63 pKa = 4.04 EE64 pKa = 4.68 DD65 pKa = 3.22 WQQMIEE71 pKa = 4.04 NMEE74 pKa = 4.2 KK75 pKa = 9.26 KK76 pKa = 8.66 TT77 pKa = 3.62
Molecular weight: 9.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.479
IPC2_protein 8.317
IPC_protein 8.448
Toseland 9.165
ProMoST 8.77
Dawson 9.355
Bjellqvist 8.96
Wikipedia 9.502
Rodwell 9.648
Grimsley 9.399
Solomon 9.531
Lehninger 9.516
Nozaki 9.033
DTASelect 9.004
Thurlkill 9.18
EMBOSS 9.531
Sillero 9.268
Patrickios 5.283
IPC_peptide 9.531
IPC2_peptide 7.527
IPC2.peptide.svr19 7.651
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1502
77
579
214.6
24.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.389 ± 1.16
0.732 ± 0.378
4.461 ± 0.605
8.322 ± 1.378
2.863 ± 0.767
7.124 ± 1.32
1.398 ± 0.472
5.459 ± 0.877
6.391 ± 1.283
6.924 ± 0.891
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.328 ± 0.494
4.66 ± 0.588
3.795 ± 0.727
4.927 ± 0.396
4.594 ± 0.718
7.19 ± 1.31
6.858 ± 0.484
4.261 ± 0.562
2.53 ± 0.481
4.794 ± 0.654
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here