Mycobacterium phage Weirdo19

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M2YSX4|A0A6M2YSX4_9CAUD Uncharacterized protein OS=Mycobacterium phage Weirdo19 OX=2601610 PE=4 SV=1
MM1 pKa = 7.13STTDD5 pKa = 3.39AAPFRR10 pKa = 11.84QVEE13 pKa = 4.12ILVDD17 pKa = 3.74GEE19 pKa = 4.34RR20 pKa = 11.84THH22 pKa = 6.86TLLASWFDD30 pKa = 3.21ITEE33 pKa = 4.55DD34 pKa = 4.83GEE36 pKa = 4.72TLTLNATRR44 pKa = 11.84WRR46 pKa = 11.84PDD48 pKa = 2.58GMIPPNPLIAEE59 pKa = 4.31VEE61 pKa = 4.41SAATSPTACSTPPNAAPCGDD81 pKa = 3.76VADD84 pKa = 4.96DD85 pKa = 5.45AFDD88 pKa = 4.52GDD90 pKa = 4.91DD91 pKa = 4.15RR92 pKa = 11.84YY93 pKa = 11.34CEE95 pKa = 3.98LTLEE99 pKa = 4.17YY100 pKa = 9.62HH101 pKa = 6.48ACVLPGEE108 pKa = 4.9HH109 pKa = 6.61IVGQAHH115 pKa = 6.34EE116 pKa = 4.7CDD118 pKa = 5.25CGVTWW123 pKa = 4.8

Molecular weight: 13.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M2YTE7|A0A6M2YTE7_9CAUD Uncharacterized protein OS=Mycobacterium phage Weirdo19 OX=2601610 PE=4 SV=1
MM1 pKa = 7.31GASQRR6 pKa = 11.84RR7 pKa = 11.84YY8 pKa = 8.75FRR10 pKa = 11.84YY11 pKa = 9.21MNSAAWRR18 pKa = 11.84ARR20 pKa = 11.84QARR23 pKa = 11.84YY24 pKa = 8.37FATHH28 pKa = 6.7PEE30 pKa = 3.9RR31 pKa = 11.84CALCGSGDD39 pKa = 3.76EE40 pKa = 4.79VEE42 pKa = 4.89LHH44 pKa = 5.39HH45 pKa = 6.06HH46 pKa = 5.72TYY48 pKa = 10.49RR49 pKa = 11.84RR50 pKa = 11.84LGAEE54 pKa = 3.96LDD56 pKa = 3.66EE57 pKa = 6.17DD58 pKa = 4.1LTALCAAHH66 pKa = 6.46HH67 pKa = 6.61AEE69 pKa = 3.71VHH71 pKa = 6.52DD72 pKa = 3.61YY73 pKa = 10.93HH74 pKa = 7.02RR75 pKa = 11.84AHH77 pKa = 7.39DD78 pKa = 3.98VTLTEE83 pKa = 3.87ATRR86 pKa = 11.84AVAARR91 pKa = 11.84FGVPIVVRR99 pKa = 11.84RR100 pKa = 11.84PRR102 pKa = 11.84TEE104 pKa = 3.8LTRR107 pKa = 11.84KK108 pKa = 7.73EE109 pKa = 3.71QNKK112 pKa = 9.18ARR114 pKa = 11.84KK115 pKa = 8.6ARR117 pKa = 11.84KK118 pKa = 8.17AQRR121 pKa = 11.84IQAQRR126 pKa = 11.84EE127 pKa = 3.96AARR130 pKa = 3.98

Molecular weight: 15.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

16768

39

1359

188.4

20.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.508 ± 0.448

1.014 ± 0.146

6.202 ± 0.23

5.833 ± 0.335

2.696 ± 0.153

9.578 ± 0.591

2.302 ± 0.199

3.876 ± 0.192

2.445 ± 0.217

8.075 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.004 ± 0.107

2.38 ± 0.195

7.168 ± 0.308

2.875 ± 0.164

8.051 ± 0.446

4.288 ± 0.215

6.459 ± 0.207

7.419 ± 0.242

1.843 ± 0.134

1.986 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski