Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Embecovirus; Murine coronavirus; Murine hepatitis virus

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P0C5A7|NS12_CVMA5 Non-structural protein of 12.7 kDa OS=Murine coronavirus (strain A59) OX=11142 GN=5a PE=3 SV=1
MM1 pKa = 7.46FNLFLTDD8 pKa = 3.48TVWYY12 pKa = 8.66VGQIIFIFAVCLMVTIIVVAFLASIKK38 pKa = 10.74LCIQLCGLCNTLVLSPSIYY57 pKa = 10.4LYY59 pKa = 10.85DD60 pKa = 4.56RR61 pKa = 11.84SKK63 pKa = 11.14QLYY66 pKa = 8.86KK67 pKa = 10.82YY68 pKa = 9.71YY69 pKa = 10.26NEE71 pKa = 3.95EE72 pKa = 3.47MRR74 pKa = 11.84LPLLEE79 pKa = 4.79VDD81 pKa = 5.76DD82 pKa = 4.29II83 pKa = 4.66

Molecular weight: 9.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P69614|IORF_CVMA5 Protein I OS=Murine coronavirus (strain A59) OX=11142 GN=N PE=3 SV=2
MM1 pKa = 7.37EE2 pKa = 4.95SSRR5 pKa = 11.84RR6 pKa = 11.84PLGLTKK12 pKa = 10.42PSVDD16 pKa = 3.44QIIKK20 pKa = 10.12IEE22 pKa = 4.14AEE24 pKa = 4.63GISQSRR30 pKa = 11.84LQLLNPTPGVWFPITPGFLALPSSKK55 pKa = 10.06RR56 pKa = 11.84EE57 pKa = 3.79RR58 pKa = 11.84SFSLQKK64 pKa = 10.79DD65 pKa = 4.05KK66 pKa = 11.56EE67 pKa = 4.45CLLPMEE73 pKa = 5.42SPLQSKK79 pKa = 10.4RR80 pKa = 11.84DD81 pKa = 3.48IGIDD85 pKa = 3.27TTAVLLKK92 pKa = 10.22HH93 pKa = 6.53LMGSRR98 pKa = 11.84SNYY101 pKa = 9.76CPDD104 pKa = 4.45GIFTILAQGPMLEE117 pKa = 4.52PVMEE121 pKa = 4.28TALKK125 pKa = 10.49VSSGLQTAKK134 pKa = 9.63RR135 pKa = 11.84TPIPALILSKK145 pKa = 10.01GTQAVMRR152 pKa = 11.84LFLLGLRR159 pKa = 11.84PARR162 pKa = 11.84YY163 pKa = 9.25CLRR166 pKa = 11.84AFMLKK171 pKa = 10.34ALEE174 pKa = 4.32GLHH177 pKa = 6.79LLADD181 pKa = 4.46LVRR184 pKa = 11.84GHH186 pKa = 6.34NPVGQIIALEE196 pKa = 4.02AVPTSASLPLLL207 pKa = 3.91

Molecular weight: 22.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

14869

83

7176

1351.7

150.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.055 ± 0.207

3.827 ± 0.32

5.596 ± 0.305

4.21 ± 0.244

5.327 ± 0.182

6.181 ± 0.369

1.728 ± 0.149

4.291 ± 0.443

5.764 ± 0.412

9.227 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.257

4.889 ± 0.533

3.82 ± 0.446

3.443 ± 0.393

3.665 ± 0.375

7.095 ± 0.295

5.683 ± 0.233

9.947 ± 1.058

1.311 ± 0.082

4.661 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski