Canid alphaherpesvirus 1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A172DSI5|A0A172DSI5_9ALPH Membrane protein UL56 OS=Canid alphaherpesvirus 1 OX=170325 GN=UL56 PE=4 SV=1
MM1 pKa = 7.76ADD3 pKa = 3.35EE4 pKa = 5.43EE5 pKa = 5.12YY6 pKa = 11.19NCTICLEE13 pKa = 4.3PPKK16 pKa = 11.06NMTVTMSCLHH26 pKa = 6.23KK27 pKa = 10.53FCYY30 pKa = 10.1DD31 pKa = 4.22CLSEE35 pKa = 4.01WTKK38 pKa = 11.0VSNTCPLCKK47 pKa = 10.29SIIQSMIHH55 pKa = 6.65SINDD59 pKa = 3.27DD60 pKa = 3.8KK61 pKa = 11.35EE62 pKa = 4.13FKK64 pKa = 9.96EE65 pKa = 3.87IKK67 pKa = 9.78IVSEE71 pKa = 4.36SIEE74 pKa = 4.48DD75 pKa = 3.78STDD78 pKa = 3.38LLMEE82 pKa = 4.48EE83 pKa = 3.88NAQRR87 pKa = 11.84FFNSDD92 pKa = 3.33EE93 pKa = 3.97EE94 pKa = 4.49DD95 pKa = 3.99ANDD98 pKa = 5.24DD99 pKa = 4.26DD100 pKa = 6.47DD101 pKa = 5.24RR102 pKa = 11.84PLWGEE107 pKa = 4.71DD108 pKa = 3.17YY109 pKa = 11.31DD110 pKa = 4.38EE111 pKa = 5.37NYY113 pKa = 10.6SIYY116 pKa = 10.18QSEE119 pKa = 4.29PASSQIQPPSHH130 pKa = 6.68NSMEE134 pKa = 4.25QILFRR139 pKa = 11.84NPNTRR144 pKa = 11.84EE145 pKa = 3.54LLIRR149 pKa = 11.84FSYY152 pKa = 9.4NRR154 pKa = 11.84LMVYY158 pKa = 10.06YY159 pKa = 9.0EE160 pKa = 4.24HH161 pKa = 7.46EE162 pKa = 4.62GNAEE166 pKa = 4.1DD167 pKa = 3.48VTEE170 pKa = 5.17IIMEE174 pKa = 5.0FIDD177 pKa = 4.99EE178 pKa = 4.37YY179 pKa = 11.65GLDD182 pKa = 3.67RR183 pKa = 11.84DD184 pKa = 4.19EE185 pKa = 4.32LTEE188 pKa = 4.05LLEE191 pKa = 4.73PLLQTYY197 pKa = 7.45TIRR200 pKa = 11.84FVNSFFTRR208 pKa = 11.84INLLSRR214 pKa = 11.84RR215 pKa = 11.84PFRR218 pKa = 11.84ISTGVQFLDD227 pKa = 5.54DD228 pKa = 6.05DD229 pKa = 6.18DD230 pKa = 7.46DD231 pKa = 7.11DD232 pKa = 7.03DD233 pKa = 6.47DD234 pKa = 4.84EE235 pKa = 6.14NSVDD239 pKa = 4.04SEE241 pKa = 4.49SDD243 pKa = 3.41SSSSLCTDD251 pKa = 4.4DD252 pKa = 5.5LTIPDD257 pKa = 4.67DD258 pKa = 4.01TQSDD262 pKa = 3.5ISDD265 pKa = 3.82LDD267 pKa = 3.49EE268 pKa = 4.5TNISTGQSVRR278 pKa = 11.84EE279 pKa = 4.02NYY281 pKa = 7.67RR282 pKa = 11.84TRR284 pKa = 11.84LRR286 pKa = 11.84SYY288 pKa = 9.4TEE290 pKa = 3.15NSTRR294 pKa = 11.84YY295 pKa = 10.05SRR297 pKa = 11.84VLGNIMSPTGGSYY310 pKa = 10.21EE311 pKa = 4.05PEE313 pKa = 3.47ISEE316 pKa = 3.96EE317 pKa = 4.21PEE319 pKa = 3.63NVEE322 pKa = 5.61IIDD325 pKa = 3.95LTIDD329 pKa = 3.29SDD331 pKa = 4.63DD332 pKa = 5.4DD333 pKa = 3.64IGG335 pKa = 4.75

Molecular weight:
38.71 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A172DSI1|A0A172DSI1_9ALPH Virion host shutoff protein OS=Canid alphaherpesvirus 1 OX=170325 GN=UL41 PE=3 SV=1
MM1 pKa = 7.82ASFNPADD8 pKa = 4.44LKK10 pKa = 10.57TITAANLRR18 pKa = 11.84GLLPAQIITIINQSNHH34 pKa = 6.29PNNASPAEE42 pKa = 3.94ILDD45 pKa = 3.57AQRR48 pKa = 11.84NLLIGTSLSMVALRR62 pKa = 11.84QRR64 pKa = 11.84HH65 pKa = 5.51ANHH68 pKa = 6.92TIARR72 pKa = 11.84VPMFAEE78 pKa = 3.9YY79 pKa = 10.6DD80 pKa = 3.68GLFWARR86 pKa = 11.84PTIGLKK92 pKa = 9.08RR93 pKa = 11.84TFSPRR98 pKa = 11.84LTQIVPDD105 pKa = 3.87EE106 pKa = 4.12

Molecular weight:
11.72 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

35638

70

3080

495.0

56.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.31 ± 0.176

1.858 ± 0.145

5.211 ± 0.185

5.794 ± 0.213

4.978 ± 0.191

4.332 ± 0.178

1.844 ± 0.084

8.623 ± 0.258

6.378 ± 0.262

9.661 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.032 ± 0.079

7.203 ± 0.2

4.737 ± 0.2

3.275 ± 0.136

4.265 ± 0.169

9.568 ± 0.273

6.075 ± 0.105

5.138 ± 0.15

0.901 ± 0.056

3.819 ± 0.153

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski