Canid alphaherpesvirus 1
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A172DSI5|A0A172DSI5_9ALPH Membrane protein UL56 OS=Canid alphaherpesvirus 1 OX=170325 GN=UL56 PE=4 SV=1
MM1 pKa = 7.76 ADD3 pKa = 3.35 EE4 pKa = 5.43 EE5 pKa = 5.12 YY6 pKa = 11.19 NCTICLEE13 pKa = 4.3 PPKK16 pKa = 11.06 NMTVTMSCLHH26 pKa = 6.23 KK27 pKa = 10.53 FCYY30 pKa = 10.1 DD31 pKa = 4.22 CLSEE35 pKa = 4.01 WTKK38 pKa = 11.0 VSNTCPLCKK47 pKa = 10.29 SIIQSMIHH55 pKa = 6.65 SINDD59 pKa = 3.27 DD60 pKa = 3.8 KK61 pKa = 11.35 EE62 pKa = 4.13 FKK64 pKa = 9.96 EE65 pKa = 3.87 IKK67 pKa = 9.78 IVSEE71 pKa = 4.36 SIEE74 pKa = 4.48 DD75 pKa = 3.78 STDD78 pKa = 3.38 LLMEE82 pKa = 4.48 EE83 pKa = 3.88 NAQRR87 pKa = 11.84 FFNSDD92 pKa = 3.33 EE93 pKa = 3.97 EE94 pKa = 4.49 DD95 pKa = 3.99 ANDD98 pKa = 5.24 DD99 pKa = 4.26 DD100 pKa = 6.47 DD101 pKa = 5.24 RR102 pKa = 11.84 PLWGEE107 pKa = 4.71 DD108 pKa = 3.17 YY109 pKa = 11.31 DD110 pKa = 4.38 EE111 pKa = 5.37 NYY113 pKa = 10.6 SIYY116 pKa = 10.18 QSEE119 pKa = 4.29 PASSQIQPPSHH130 pKa = 6.68 NSMEE134 pKa = 4.25 QILFRR139 pKa = 11.84 NPNTRR144 pKa = 11.84 EE145 pKa = 3.54 LLIRR149 pKa = 11.84 FSYY152 pKa = 9.4 NRR154 pKa = 11.84 LMVYY158 pKa = 10.06 YY159 pKa = 9.0 EE160 pKa = 4.24 HH161 pKa = 7.46 EE162 pKa = 4.62 GNAEE166 pKa = 4.1 DD167 pKa = 3.48 VTEE170 pKa = 5.17 IIMEE174 pKa = 5.0 FIDD177 pKa = 4.99 EE178 pKa = 4.37 YY179 pKa = 11.65 GLDD182 pKa = 3.67 RR183 pKa = 11.84 DD184 pKa = 4.19 EE185 pKa = 4.32 LTEE188 pKa = 4.05 LLEE191 pKa = 4.73 PLLQTYY197 pKa = 7.45 TIRR200 pKa = 11.84 FVNSFFTRR208 pKa = 11.84 INLLSRR214 pKa = 11.84 RR215 pKa = 11.84 PFRR218 pKa = 11.84 ISTGVQFLDD227 pKa = 5.54 DD228 pKa = 6.05 DD229 pKa = 6.18 DD230 pKa = 7.46 DD231 pKa = 7.11 DD232 pKa = 7.03 DD233 pKa = 6.47 DD234 pKa = 4.84 EE235 pKa = 6.14 NSVDD239 pKa = 4.04 SEE241 pKa = 4.49 SDD243 pKa = 3.41 SSSSLCTDD251 pKa = 4.4 DD252 pKa = 5.5 LTIPDD257 pKa = 4.67 DD258 pKa = 4.01 TQSDD262 pKa = 3.5 ISDD265 pKa = 3.82 LDD267 pKa = 3.49 EE268 pKa = 4.5 TNISTGQSVRR278 pKa = 11.84 EE279 pKa = 4.02 NYY281 pKa = 7.67 RR282 pKa = 11.84 TRR284 pKa = 11.84 LRR286 pKa = 11.84 SYY288 pKa = 9.4 TEE290 pKa = 3.15 NSTRR294 pKa = 11.84 YY295 pKa = 10.05 SRR297 pKa = 11.84 VLGNIMSPTGGSYY310 pKa = 10.21 EE311 pKa = 4.05 PEE313 pKa = 3.47 ISEE316 pKa = 3.96 EE317 pKa = 4.21 PEE319 pKa = 3.63 NVEE322 pKa = 5.61 IIDD325 pKa = 3.95 LTIDD329 pKa = 3.29 SDD331 pKa = 4.63 DD332 pKa = 5.4 DD333 pKa = 3.64 IGG335 pKa = 4.75
Molecular weight: 38.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.983
IPC2_protein 3.884
IPC_protein 3.897
Toseland 3.681
ProMoST 4.024
Dawson 3.872
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.592
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.177
Thurlkill 3.719
EMBOSS 3.783
Sillero 4.012
Patrickios 2.931
IPC_peptide 3.859
IPC2_peptide 3.986
IPC2.peptide.svr19 3.916
Protein with the highest isoelectric point:
>tr|A0A172DSI1|A0A172DSI1_9ALPH Virion host shutoff protein OS=Canid alphaherpesvirus 1 OX=170325 GN=UL41 PE=3 SV=1
MM1 pKa = 7.82 ASFNPADD8 pKa = 4.44 LKK10 pKa = 10.57 TITAANLRR18 pKa = 11.84 GLLPAQIITIINQSNHH34 pKa = 6.29 PNNASPAEE42 pKa = 3.94 ILDD45 pKa = 3.57 AQRR48 pKa = 11.84 NLLIGTSLSMVALRR62 pKa = 11.84 QRR64 pKa = 11.84 HH65 pKa = 5.51 ANHH68 pKa = 6.92 TIARR72 pKa = 11.84 VPMFAEE78 pKa = 3.9 YY79 pKa = 10.6 DD80 pKa = 3.68 GLFWARR86 pKa = 11.84 PTIGLKK92 pKa = 9.08 RR93 pKa = 11.84 TFSPRR98 pKa = 11.84 LTQIVPDD105 pKa = 3.87 EE106 pKa = 4.12
Molecular weight: 11.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.273
IPC2_protein 9.326
IPC_protein 10.072
Toseland 10.409
ProMoST 10.101
Dawson 10.526
Bjellqvist 10.218
Wikipedia 10.73
Rodwell 10.643
Grimsley 10.57
Solomon 10.657
Lehninger 10.643
Nozaki 10.365
DTASelect 10.218
Thurlkill 10.409
EMBOSS 10.804
Sillero 10.452
Patrickios 10.54
IPC_peptide 10.657
IPC2_peptide 8.931
IPC2.peptide.svr19 8.699
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
35638
70
3080
495.0
56.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.31 ± 0.176
1.858 ± 0.145
5.211 ± 0.185
5.794 ± 0.213
4.978 ± 0.191
4.332 ± 0.178
1.844 ± 0.084
8.623 ± 0.258
6.378 ± 0.262
9.661 ± 0.215
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.032 ± 0.079
7.203 ± 0.2
4.737 ± 0.2
3.275 ± 0.136
4.265 ± 0.169
9.568 ± 0.273
6.075 ± 0.105
5.138 ± 0.15
0.901 ± 0.056
3.819 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here