Streptococcus phage IPP28

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SAR4|A0A1S5SAR4_9CAUD Uncharacterized protein OS=Streptococcus phage IPP28 OX=1916168 GN=IPP28_00042 PE=4 SV=1
MM1 pKa = 7.37ILKK4 pKa = 9.85FRR6 pKa = 11.84VWHH9 pKa = 6.49HH10 pKa = 4.98EE11 pKa = 3.66LGRR14 pKa = 11.84LMSVKK19 pKa = 10.46CMFFQDD25 pKa = 3.77SEE27 pKa = 4.43IEE29 pKa = 4.12EE30 pKa = 4.53FEE32 pKa = 5.65LNDD35 pKa = 3.79TLMNDD40 pKa = 4.39YY41 pKa = 9.15ITAYY45 pKa = 9.2PDD47 pKa = 3.84EE48 pKa = 5.96IEE50 pKa = 5.04LMQSTGILDD59 pKa = 3.7KK60 pKa = 11.18NGKK63 pKa = 8.27EE64 pKa = 4.06VFEE67 pKa = 4.21GDD69 pKa = 2.68IVRR72 pKa = 11.84FFDD75 pKa = 3.17SLYY78 pKa = 10.49TVFYY82 pKa = 10.0DD83 pKa = 4.7IKK85 pKa = 10.87DD86 pKa = 3.47RR87 pKa = 11.84WVVDD91 pKa = 3.16YY92 pKa = 9.58MSNFSSEE99 pKa = 3.87EE100 pKa = 3.91SIEE103 pKa = 4.21IIGNIYY109 pKa = 10.06EE110 pKa = 4.02NLVYY114 pKa = 10.53IFF116 pKa = 4.7

Molecular weight: 13.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SAQ6|A0A1S5SAQ6_9CAUD Putative terminase large subunit OS=Streptococcus phage IPP28 OX=1916168 GN=IPP28_00033 PE=4 SV=1
MM1 pKa = 7.48EE2 pKa = 4.94FVSPIKK8 pKa = 11.06DD9 pKa = 3.42NDD11 pKa = 4.55DD12 pKa = 3.23IQAMKK17 pKa = 10.62DD18 pKa = 3.6YY19 pKa = 10.67LRR21 pKa = 11.84EE22 pKa = 4.13WNEE25 pKa = 3.47MYY27 pKa = 11.37YY28 pKa = 9.99MLFITGLNTGLRR40 pKa = 11.84VGDD43 pKa = 3.65ILTLKK48 pKa = 10.66VKK50 pKa = 10.31DD51 pKa = 3.59VQGWHH56 pKa = 5.33IKK58 pKa = 9.99LRR60 pKa = 11.84EE61 pKa = 4.04RR62 pKa = 11.84KK63 pKa = 7.72TGKK66 pKa = 10.21QITRR70 pKa = 11.84RR71 pKa = 11.84MTKK74 pKa = 8.23EE75 pKa = 3.51LKK77 pKa = 10.21KK78 pKa = 10.38EE79 pKa = 3.45MRR81 pKa = 11.84RR82 pKa = 11.84YY83 pKa = 9.81VEE85 pKa = 4.35GKK87 pKa = 9.12PFHH90 pKa = 6.84HH91 pKa = 6.93FLFKK95 pKa = 10.72SRR97 pKa = 11.84QGQNKK102 pKa = 9.82AITRR106 pKa = 11.84EE107 pKa = 3.48RR108 pKa = 11.84AYY110 pKa = 10.73QIIHH114 pKa = 6.19EE115 pKa = 4.43AAEE118 pKa = 3.91EE119 pKa = 3.97LGIDD123 pKa = 3.49NVGTHH128 pKa = 5.72TMRR131 pKa = 11.84KK132 pKa = 6.62TFGYY136 pKa = 10.16KK137 pKa = 10.08YY138 pKa = 9.91YY139 pKa = 11.16NKK141 pKa = 9.47TKK143 pKa = 10.72DD144 pKa = 3.11VGTLQKK150 pKa = 9.79MFNHH154 pKa = 6.53SSPAITLRR162 pKa = 11.84YY163 pKa = 9.45IGIEE167 pKa = 3.91QAEE170 pKa = 4.45LDD172 pKa = 3.58DD173 pKa = 4.2ALRR176 pKa = 11.84NFVII180 pKa = 4.81

Molecular weight: 21.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10324

34

1954

198.5

22.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.809 ± 0.514

0.542 ± 0.111

5.86 ± 0.339

8.262 ± 0.371

4.388 ± 0.267

5.821 ± 0.303

1.472 ± 0.2

7.022 ± 0.31

8.33 ± 0.39

8.727 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.528 ± 0.274

5.599 ± 0.294

2.615 ± 0.217

4.175 ± 0.369

4.717 ± 0.29

5.938 ± 0.511

6.005 ± 0.345

6.306 ± 0.247

1.395 ± 0.129

3.487 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski