Shrimp hemocyte iridescent virus
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 170 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A291B0Y7|A0A291B0Y7_9VIRU Uncharacterized protein OS=Shrimp hemocyte iridescent virus OX=2039780 GN=147R PE=4 SV=1
MM1 pKa = 7.64 FNNCVLKK8 pKa = 10.78 AVFSDD13 pKa = 3.52 DD14 pKa = 3.94 TIEE17 pKa = 4.16 KK18 pKa = 10.9 LEE20 pKa = 4.18 MLDD23 pKa = 2.86 ITLPTKK29 pKa = 10.84 LNFVGGEE36 pKa = 4.12 YY37 pKa = 10.96 YY38 pKa = 10.67 CISRR42 pKa = 11.84 NNIISSTAEE51 pKa = 3.87 LQLTTSKK58 pKa = 10.18 IQYY61 pKa = 8.99 AKK63 pKa = 10.27 IVIFSCMFGIGKK75 pKa = 9.19 EE76 pKa = 4.21 CVIEE80 pKa = 4.62 DD81 pKa = 4.68 DD82 pKa = 4.01 GTVIPFNSQPTQVFQIDD99 pKa = 4.34 DD100 pKa = 3.8 IFQEE104 pKa = 4.74 EE105 pKa = 4.73 YY106 pKa = 10.6 LLTMSRR112 pKa = 11.84 SHH114 pKa = 6.61 MDD116 pKa = 3.67 SIVSDD121 pKa = 3.85 YY122 pKa = 11.31 QLLLDD127 pKa = 4.21 YY128 pKa = 11.38 LCDD131 pKa = 3.63 NNMMDD136 pKa = 4.51 EE137 pKa = 4.34 FTKK140 pKa = 10.78 SEE142 pKa = 5.16 LIFKK146 pKa = 10.05 LQTLANQCIDD156 pKa = 3.62 FEE158 pKa = 4.54 NPQEE162 pKa = 4.28 PEE164 pKa = 3.58 EE165 pKa = 4.98 HH166 pKa = 6.69 EE167 pKa = 4.2 FQEE170 pKa = 5.01 DD171 pKa = 3.08 SGYY174 pKa = 11.11 EE175 pKa = 4.01 DD176 pKa = 3.73 NSHH179 pKa = 6.22 NEE181 pKa = 3.42 WWW183 pKa = 3.44
Molecular weight: 21.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.805
IPC2_protein 3.999
IPC_protein 3.961
Toseland 3.77
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.821
Rodwell 3.795
Grimsley 3.681
Solomon 3.923
Lehninger 3.872
Nozaki 4.037
DTASelect 4.215
Thurlkill 3.808
EMBOSS 3.834
Sillero 4.075
Patrickios 0.846
IPC_peptide 3.923
IPC2_peptide 4.062
IPC2.peptide.svr19 3.96
Protein with the highest isoelectric point:
>tr|A0A291B0N4|A0A291B0N4_9VIRU Uncharacterized protein OS=Shrimp hemocyte iridescent virus OX=2039780 GN=46R PE=4 SV=1
MM1 pKa = 7.81 GPFLPTNEE9 pKa = 5.23 IIPGMSPSRR18 pKa = 11.84 VRR20 pKa = 11.84 RR21 pKa = 11.84 IQQKK25 pKa = 7.71 YY26 pKa = 4.34 TTPKK30 pKa = 10.35 RR31 pKa = 11.84 PSTYY35 pKa = 8.68 YY36 pKa = 10.38 DD37 pKa = 2.98 ARR39 pKa = 11.84 SRR41 pKa = 11.84 PFKK44 pKa = 9.73 PANDD48 pKa = 4.59 IIPGMSAAEE57 pKa = 3.83 VRR59 pKa = 11.84 RR60 pKa = 11.84 IKK62 pKa = 10.55 KK63 pKa = 10.13 KK64 pKa = 8.76 YY65 pKa = 8.25 AQPAEE70 pKa = 3.83 EE71 pKa = 4.35 SVYY74 pKa = 10.75 FDD76 pKa = 5.62 ALDD79 pKa = 3.92 LDD81 pKa = 4.23
Molecular weight: 9.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.15
IPC2_protein 9.282
IPC_protein 9.399
Toseland 9.78
ProMoST 9.575
Dawson 10.043
Bjellqvist 9.75
Wikipedia 10.248
Rodwell 10.306
Grimsley 10.145
Solomon 10.101
Lehninger 10.058
Nozaki 9.736
DTASelect 9.75
Thurlkill 9.867
EMBOSS 10.204
Sillero 9.955
Patrickios 7.614
IPC_peptide 10.087
IPC2_peptide 8.346
IPC2.peptide.svr19 8.306
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
170
0
170
49110
21
1167
288.9
33.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.612 ± 0.138
1.69 ± 0.092
6.241 ± 0.137
8.414 ± 0.204
5.343 ± 0.139
4.292 ± 0.145
1.765 ± 0.072
8.045 ± 0.126
9.636 ± 0.21
7.807 ± 0.142
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.792 ± 0.087
6.728 ± 0.137
3.944 ± 0.171
3.437 ± 0.137
4.311 ± 0.175
6.373 ± 0.168
5.054 ± 0.201
5.661 ± 0.114
0.829 ± 0.05
4.026 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here