Clostridium sp. CAG:921

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1350 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5KZ07|R5KZ07_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:921 OX=1262847 GN=BN811_00092 PE=4 SV=1
MM1 pKa = 7.89IYY3 pKa = 8.89NTNKK7 pKa = 10.52LLDD10 pKa = 3.93IEE12 pKa = 4.65NGIIDD17 pKa = 3.91GPVNKK22 pKa = 9.55FLHH25 pKa = 6.62EE26 pKa = 4.11YY27 pKa = 10.95EE28 pKa = 4.41EE29 pKa = 5.32DD30 pKa = 3.78NEE32 pKa = 4.14NDD34 pKa = 3.38EE35 pKa = 4.2EE36 pKa = 4.24MKK38 pKa = 10.88EE39 pKa = 3.97IVNDD43 pKa = 3.29INNYY47 pKa = 8.63LTFSMITNSLALIIMIIIVILYY69 pKa = 9.36AFNAMM74 pKa = 4.23

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5KY77|R5KY77_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:921 OX=1262847 GN=BN811_01138 PE=4 SV=1
MM1 pKa = 7.59AKK3 pKa = 10.28KK4 pKa = 10.86SMIAKK9 pKa = 7.99QQRR12 pKa = 11.84EE13 pKa = 4.37PKK15 pKa = 10.41FSTRR19 pKa = 11.84YY20 pKa = 9.02YY21 pKa = 10.38NRR23 pKa = 11.84CKK25 pKa = 10.18ICGRR29 pKa = 11.84PHH31 pKa = 7.87SYY33 pKa = 8.05MRR35 pKa = 11.84KK36 pKa = 8.84YY37 pKa = 10.37GVCRR41 pKa = 11.84LCFRR45 pKa = 11.84KK46 pKa = 9.55LAYY49 pKa = 9.88QGEE52 pKa = 4.36IPGVKK57 pKa = 9.33KK58 pKa = 10.94ASWW61 pKa = 3.03

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1350

0

1350

360185

29

1884

266.8

30.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.798 ± 0.06

1.28 ± 0.028

5.868 ± 0.054

7.399 ± 0.079

4.444 ± 0.059

5.245 ± 0.068

1.091 ± 0.023

9.965 ± 0.081

10.085 ± 0.069

8.48 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.493 ± 0.035

7.209 ± 0.081

2.259 ± 0.03

2.371 ± 0.037

3.132 ± 0.052

6.858 ± 0.071

5.093 ± 0.061

6.622 ± 0.07

0.513 ± 0.016

4.772 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski