Phytophthora infestans RNA virus 1
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7SHT6|C7SHT6_9VIRU Uncharacterized protein ORF2A OS=Phytophthora infestans RNA virus 1 OX=640897 GN=ORF2A PE=4 SV=1
MM1 pKa = 7.22 QINVGGRR8 pKa = 11.84 EE9 pKa = 3.7 LCAIFVGLICWMFICEE25 pKa = 4.76 FIVNEE30 pKa = 3.97 YY31 pKa = 9.04 EE32 pKa = 3.89 QFYY35 pKa = 8.08 GTEE38 pKa = 4.32 YY39 pKa = 10.35 TVEE42 pKa = 4.12 TSPMCALFEE51 pKa = 4.47 GCSLNVEE58 pKa = 4.26 SFLKK62 pKa = 11.06 NEE64 pKa = 4.26 DD65 pKa = 3.15 VRR67 pKa = 11.84 SIMITALFVGKK78 pKa = 8.9 MLEE81 pKa = 4.1 MLDD84 pKa = 3.21 ITVKK88 pKa = 10.86 FEE90 pKa = 3.84 FSSAEE95 pKa = 3.76 EE96 pKa = 4.54 GEE98 pKa = 4.87 SVFADD103 pKa = 4.54 FNRR106 pKa = 11.84 DD107 pKa = 3.05 LRR109 pKa = 11.84 DD110 pKa = 3.17 VRR112 pKa = 11.84 RR113 pKa = 11.84 SVEE116 pKa = 4.06 LKK118 pKa = 9.83 ASSMKK123 pKa = 10.49 KK124 pKa = 9.79 IIADD128 pKa = 3.66 FRR130 pKa = 11.84 TKK132 pKa = 10.01 IRR134 pKa = 11.84 SIVYY138 pKa = 10.01 LGVSNIDD145 pKa = 3.31 IPNVHH150 pKa = 6.84 AMRR153 pKa = 11.84 YY154 pKa = 9.06 PIDD157 pKa = 3.63 FFMSRR162 pKa = 11.84 LYY164 pKa = 10.87 RR165 pKa = 11.84 MKK167 pKa = 10.19 FLKK170 pKa = 10.26 EE171 pKa = 3.92 YY172 pKa = 10.24 EE173 pKa = 4.16 EE174 pKa = 4.12 SRR176 pKa = 11.84 EE177 pKa = 3.79 KK178 pKa = 10.56 YY179 pKa = 9.96 RR180 pKa = 11.84 RR181 pKa = 11.84 VNPTLAMKK189 pKa = 10.21 LAQLLRR195 pKa = 11.84 YY196 pKa = 10.05 DD197 pKa = 4.02 NEE199 pKa = 4.29 FLLEE203 pKa = 4.28 SNVAEE208 pKa = 4.05 ALKK211 pKa = 10.71 EE212 pKa = 4.16 SVEE215 pKa = 4.22 EE216 pKa = 3.87 VLEE219 pKa = 4.3 EE220 pKa = 4.3 VFSNPSFEE228 pKa = 4.16 EE229 pKa = 3.86 VKK231 pKa = 10.48 IEE233 pKa = 4.38 LNGRR237 pKa = 11.84 LNSGMAMFDD246 pKa = 4.23 DD247 pKa = 4.21 INVNGSSAAGYY258 pKa = 7.42 PYY260 pKa = 9.8 PAGVKK265 pKa = 9.99 RR266 pKa = 11.84 RR267 pKa = 11.84 DD268 pKa = 3.48 VLEE271 pKa = 3.99 EE272 pKa = 4.1 AKK274 pKa = 10.8 ASAYY278 pKa = 10.4 DD279 pKa = 4.13 LYY281 pKa = 11.42 NDD283 pKa = 3.39 SDD285 pKa = 4.02 LFEE288 pKa = 7.53 GYY290 pKa = 9.63 MSDD293 pKa = 3.48 HH294 pKa = 6.57 RR295 pKa = 11.84 WYY297 pKa = 7.24 TTGRR301 pKa = 11.84 AKK303 pKa = 10.13 LVKK306 pKa = 9.42 MGKK309 pKa = 8.53 PDD311 pKa = 3.46 KK312 pKa = 10.87 ARR314 pKa = 11.84 LVLYY318 pKa = 10.82 SGFSYY323 pKa = 11.15 SLLGFVYY330 pKa = 10.05 SQVWTGFMNRR340 pKa = 11.84 QCRR343 pKa = 11.84 GWSAVGMSWMNGGAAKK359 pKa = 10.17 VASFFEE365 pKa = 4.65 DD366 pKa = 3.62 CFGIAVSGFEE376 pKa = 4.46 YY377 pKa = 8.23 MTLDD381 pKa = 3.29 VAEE384 pKa = 4.58 WDD386 pKa = 3.61 SSVCRR391 pKa = 11.84 EE392 pKa = 4.46 LLHH395 pKa = 5.85 ACKK398 pKa = 9.83 RR399 pKa = 11.84 FHH401 pKa = 6.16 MRR403 pKa = 11.84 VLEE406 pKa = 4.14 RR407 pKa = 11.84 TLSPEE412 pKa = 3.36 NARR415 pKa = 11.84 YY416 pKa = 9.53 KK417 pKa = 10.56 EE418 pKa = 4.14 YY419 pKa = 10.52 FGRR422 pKa = 11.84 IYY424 pKa = 11.05 DD425 pKa = 3.74 EE426 pKa = 4.38 MIEE429 pKa = 4.25 AKK431 pKa = 10.25 VVLPGGHH438 pKa = 5.95 SFRR441 pKa = 11.84 LHH443 pKa = 7.09 HH444 pKa = 6.12 GMKK447 pKa = 10.21 SGWIMTANDD456 pKa = 3.55 NTLMHH461 pKa = 6.56 EE462 pKa = 4.36 FVVRR466 pKa = 11.84 TLQKK470 pKa = 10.04 IGQIPDD476 pKa = 3.52 MKK478 pKa = 10.2 RR479 pKa = 11.84 QLYY482 pKa = 10.78 GDD484 pKa = 4.78 DD485 pKa = 3.51 NLSRR489 pKa = 11.84 KK490 pKa = 8.97 PIGMSKK496 pKa = 9.94 QLLVDD501 pKa = 4.25 GYY503 pKa = 11.63 GMFGFRR509 pKa = 11.84 LSHH512 pKa = 5.33 IHH514 pKa = 4.54 VSRR517 pKa = 11.84 RR518 pKa = 11.84 LSEE521 pKa = 3.97 VDD523 pKa = 4.03 FLSKK527 pKa = 10.31 FIIFKK532 pKa = 10.69 DD533 pKa = 3.4 GFYY536 pKa = 10.64 FPWRR540 pKa = 11.84 EE541 pKa = 3.7 QTEE544 pKa = 4.17 THH546 pKa = 6.22 ARR548 pKa = 11.84 LLMPEE553 pKa = 4.28 EE554 pKa = 4.16 FDD556 pKa = 3.47 PNYY559 pKa = 10.54 RR560 pKa = 11.84 IVPDD564 pKa = 3.39 SRR566 pKa = 11.84 VAAEE570 pKa = 4.32 HH571 pKa = 6.64 LLGHH575 pKa = 7.32 LLDD578 pKa = 4.58 NPFNANVRR586 pKa = 11.84 HH587 pKa = 5.16 VCFTLLEE594 pKa = 4.43 YY595 pKa = 10.39 IKK597 pKa = 10.82 SNYY600 pKa = 8.21 GVRR603 pKa = 11.84 TINVEE608 pKa = 3.84 EE609 pKa = 4.71 FINTRR614 pKa = 11.84 WSFMLRR620 pKa = 11.84 DD621 pKa = 3.82 IKK623 pKa = 10.62 RR624 pKa = 11.84 LCGEE628 pKa = 4.02 IPTVPSVEE636 pKa = 5.06 FIQEE640 pKa = 4.14 LYY642 pKa = 11.08 GVFSEE647 pKa = 4.28 PVSFNWAGVGEE658 pKa = 4.44 YY659 pKa = 10.32 ARR661 pKa = 11.84 KK662 pKa = 9.58 LPKK665 pKa = 10.15 FSFISKK671 pKa = 10.12 APSAEE676 pKa = 3.85 PFFRR680 pKa = 11.84 SQKK683 pKa = 10.52 AAFEE687 pKa = 4.43 LSVSEE692 pKa = 4.3 KK693 pKa = 10.09 FGHH696 pKa = 6.58 RR697 pKa = 11.84 KK698 pKa = 7.53 TSALNRR704 pKa = 11.84 ACGYY708 pKa = 10.91 KK709 pKa = 10.31 KK710 pKa = 9.95 MPLHH714 pKa = 6.87 IFGQAGGKK722 pKa = 9.62 LIEE725 pKa = 5.17 IINTCGLKK733 pKa = 10.53 CEE735 pKa = 4.33 SVLDD739 pKa = 4.51 LGTHH743 pKa = 7.07 PGAAMATLQSMHH755 pKa = 6.29 KK756 pKa = 9.55 VKK758 pKa = 10.77 SATCVSLFPEE768 pKa = 4.06 IDD770 pKa = 3.31 KK771 pKa = 11.52 SKK773 pKa = 10.43 GFCPRR778 pKa = 11.84 VLRR781 pKa = 11.84 GKK783 pKa = 10.12 DD784 pKa = 3.37 FEE786 pKa = 4.22 FFEE789 pKa = 4.44 MSAEE793 pKa = 3.97 KK794 pKa = 10.33 FKK796 pKa = 9.59 PTKK799 pKa = 10.34 HH800 pKa = 6.68 YY801 pKa = 11.32 DD802 pKa = 3.0 MCFDD806 pKa = 5.88 DD807 pKa = 5.78 IYY809 pKa = 11.49 DD810 pKa = 3.62 FEE812 pKa = 4.24 EE813 pKa = 4.41 ADD815 pKa = 3.42 RR816 pKa = 11.84 FKK818 pKa = 10.8 RR819 pKa = 11.84 AEE821 pKa = 3.77 QMFGHH826 pKa = 6.62 VDD828 pKa = 2.98 RR829 pKa = 11.84 HH830 pKa = 5.06 FAGALTRR837 pKa = 11.84 AEE839 pKa = 4.23 KK840 pKa = 10.41 FKK842 pKa = 10.2 PFCGSYY848 pKa = 9.33 VMKK851 pKa = 10.77 VRR853 pKa = 11.84 GFTPLVLKK861 pKa = 10.54 GLYY864 pKa = 9.79 DD865 pKa = 4.3 LYY867 pKa = 11.55 RR868 pKa = 11.84 MWGYY872 pKa = 11.38 LDD874 pKa = 3.16 IRR876 pKa = 11.84 KK877 pKa = 7.53 TFYY880 pKa = 11.02 SNPWNPEE887 pKa = 3.7 FYY889 pKa = 9.96 VVLVKK894 pKa = 10.73 RR895 pKa = 11.84 HH896 pKa = 5.14 DD897 pKa = 3.48 QYY899 pKa = 11.8 VRR901 pKa = 11.84 RR902 pKa = 11.84 STFNGAVNAYY912 pKa = 10.1 LNGIAPRR919 pKa = 11.84 LSFFVNARR927 pKa = 11.84 AFNYY931 pKa = 10.54 DD932 pKa = 3.04 RR933 pKa = 11.84 MSLGRR938 pKa = 11.84 EE939 pKa = 3.91 TLNNPLRR946 pKa = 11.84 DD947 pKa = 3.84 DD948 pKa = 3.75 EE949 pKa = 4.64 TVQRR953 pKa = 11.84 AFDD956 pKa = 3.97 AYY958 pKa = 10.82 AEE960 pKa = 4.23 TLRR963 pKa = 11.84 AQSFNDD969 pKa = 3.85 DD970 pKa = 3.8 YY971 pKa = 10.29 PTEE974 pKa = 4.14 VMFF977 pKa = 5.93
Molecular weight: 112.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.516
IPC2_protein 6.678
IPC_protein 6.678
Toseland 6.678
ProMoST 7.132
Dawson 7.19
Bjellqvist 7.278
Wikipedia 7.146
Rodwell 7.19
Grimsley 6.781
Solomon 7.205
Lehninger 7.205
Nozaki 7.556
DTASelect 7.483
Thurlkill 7.527
EMBOSS 7.527
Sillero 7.644
Patrickios 4.215
IPC_peptide 7.205
IPC2_peptide 7.044
IPC2.peptide.svr19 6.889
Protein with the highest isoelectric point:
>tr|C7SHT7|C7SHT7_9VIRU Uncharacterized protein ORF3A OS=Phytophthora infestans RNA virus 1 OX=640897 GN=ORF3A PE=4 SV=1
MM1 pKa = 7.59 VSEE4 pKa = 4.59 VVLVDD9 pKa = 3.55 YY10 pKa = 11.24 SRR12 pKa = 11.84 DD13 pKa = 3.16 RR14 pKa = 11.84 HH15 pKa = 5.28 AVTRR19 pKa = 11.84 AAQWVFSLPARR30 pKa = 11.84 LDD32 pKa = 4.09 GVCPWVRR39 pKa = 11.84 EE40 pKa = 4.24 EE41 pKa = 4.33 EE42 pKa = 4.26 CSLLCQVICFFRR54 pKa = 11.84 WLAEE58 pKa = 3.7 WKK60 pKa = 10.15 RR61 pKa = 11.84 WVACLLPSNEE71 pKa = 3.73 VRR73 pKa = 11.84 GMRR76 pKa = 11.84 TEE78 pKa = 3.86 IGVQEE83 pKa = 4.21 AQLGVNSPINRR94 pKa = 11.84 SWMEE98 pKa = 3.82 LRR100 pKa = 11.84 SLAVFVSVIVCGVILSVIGFLRR122 pKa = 11.84 HH123 pKa = 5.31 RR124 pKa = 11.84 RR125 pKa = 11.84 ALRR128 pKa = 11.84 STRR131 pKa = 11.84 LLTVMGLAQLLIVTIAGMTVTLTDD155 pKa = 3.93 FLCVISLMEE164 pKa = 4.24 LVTLEE169 pKa = 4.08 MAVLLEE175 pKa = 4.1 SFLEE179 pKa = 4.29 FFEE182 pKa = 4.02 MRR184 pKa = 11.84 RR185 pKa = 11.84 NRR187 pKa = 11.84 LVPVSISS194 pKa = 2.98
Molecular weight: 22.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.24
IPC2_protein 7.19
IPC_protein 7.176
Toseland 6.664
ProMoST 7.6
Dawson 7.819
Bjellqvist 8.244
Wikipedia 7.717
Rodwell 7.819
Grimsley 6.649
Solomon 7.907
Lehninger 7.936
Nozaki 8.682
DTASelect 7.966
Thurlkill 8.009
EMBOSS 8.053
Sillero 8.39
Patrickios 4.533
IPC_peptide 7.892
IPC2_peptide 8.375
IPC2.peptide.svr19 8.328
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2018
194
977
672.7
75.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.938 ± 0.418
2.23 ± 0.316
4.559 ± 0.519
6.541 ± 0.856
7.433 ± 0.522
7.83 ± 1.459
1.685 ± 0.379
5.104 ± 0.494
4.609 ± 0.971
9.713 ± 1.079
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.072 ± 0.744
4.014 ± 0.37
2.924 ± 0.449
1.685 ± 0.236
6.739 ± 0.666
7.136 ± 0.14
3.865 ± 0.208
8.87 ± 1.391
1.437 ± 0.31
3.617 ± 0.717
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here