Sanxia tombus-like virus 7
Average proteome isoelectric point is 8.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KGC2|A0A1L3KGC2_9VIRU Uncharacterized protein OS=Sanxia tombus-like virus 7 OX=1923391 PE=4 SV=1
MM1 pKa = 7.35 TARR4 pKa = 11.84 CVLAMPRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 GTATNGAGYY22 pKa = 10.78 APVSLGTGVRR32 pKa = 11.84 ANRR35 pKa = 11.84 RR36 pKa = 11.84 TPPMQLEE43 pKa = 4.23 GTDD46 pKa = 3.62 RR47 pKa = 11.84 IGVINWDD54 pKa = 3.47 GALDD58 pKa = 3.78 TGHH61 pKa = 6.92 VLFDD65 pKa = 4.23 LLVEE69 pKa = 4.2 PGIFKK74 pKa = 10.6 SLRR77 pKa = 11.84 DD78 pKa = 3.52 VAKK81 pKa = 10.41 ARR83 pKa = 11.84 QYY85 pKa = 9.87 IQWHH89 pKa = 5.12 ALEE92 pKa = 5.26 FKK94 pKa = 10.25 FSCGTNTAKK103 pKa = 10.76 DD104 pKa = 3.84 GSYY107 pKa = 10.38 VAAFIADD114 pKa = 4.17 PDD116 pKa = 4.58 DD117 pKa = 5.0 KK118 pKa = 11.76 NPDD121 pKa = 3.76 DD122 pKa = 3.69 PTLAVEE128 pKa = 4.81 EE129 pKa = 4.44 VAGAPGMVQDD139 pKa = 4.69 SIWRR143 pKa = 11.84 TRR145 pKa = 11.84 TVSVGRR151 pKa = 11.84 GRR153 pKa = 11.84 GNSTQNAIVDD163 pKa = 3.73 PTKK166 pKa = 10.36 YY167 pKa = 10.55 GHH169 pKa = 5.44 YY170 pKa = 8.98 TSTAGEE176 pKa = 3.97 PRR178 pKa = 11.84 FYY180 pKa = 11.2 SPGSIRR186 pKa = 11.84 ALVDD190 pKa = 3.12 GRR192 pKa = 11.84 VGQAGTMVLYY202 pKa = 8.63 CTYY205 pKa = 10.65 RR206 pKa = 11.84 CTLHH210 pKa = 5.8 VQARR214 pKa = 11.84 QDD216 pKa = 3.75 DD217 pKa = 4.42 AQLAPAPVIRR227 pKa = 11.84 NVFPWVWPDD236 pKa = 3.26 DD237 pKa = 3.53 TDD239 pKa = 4.54 YY240 pKa = 11.61 AITDD244 pKa = 4.15 PGPNGVVIRR253 pKa = 11.84 WDD255 pKa = 3.46 DD256 pKa = 3.46 SFPEE260 pKa = 4.31 YY261 pKa = 10.42 EE262 pKa = 4.82 RR263 pKa = 11.84 PTTDD267 pKa = 2.37 TVFQVPVPVVCSAKK281 pKa = 9.18 GTNAGGRR288 pKa = 11.84 VICPYY293 pKa = 10.37 ILYY296 pKa = 10.07 QPSSDD301 pKa = 3.51 QFYY304 pKa = 8.11 MTDD307 pKa = 4.03 DD308 pKa = 3.94 LDD310 pKa = 4.14 GAAVLQATSPSAASVFQIMPAGMTFTVVYY339 pKa = 9.9 TEE341 pKa = 4.57 GEE343 pKa = 4.16 VTRR346 pKa = 11.84 GVRR349 pKa = 11.84 EE350 pKa = 3.99 LPGGGLTNRR359 pKa = 11.84 PPAPRR364 pKa = 11.84 GIRR367 pKa = 11.84 RR368 pKa = 11.84 KK369 pKa = 10.24 AYY371 pKa = 9.79
Molecular weight: 40.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.013
IPC2_protein 6.071
IPC_protein 6.16
Toseland 6.287
ProMoST 6.576
Dawson 6.491
Bjellqvist 6.44
Wikipedia 6.504
Rodwell 6.478
Grimsley 6.364
Solomon 6.504
Lehninger 6.491
Nozaki 6.737
DTASelect 6.912
Thurlkill 6.912
EMBOSS 6.898
Sillero 6.868
Patrickios 4.088
IPC_peptide 6.517
IPC2_peptide 6.751
IPC2.peptide.svr19 6.702
Protein with the highest isoelectric point:
>tr|A0A1L3KGH8|A0A1L3KGH8_9VIRU Uncharacterized protein OS=Sanxia tombus-like virus 7 OX=1923391 PE=4 SV=1
MM1 pKa = 7.4 ATTAQAGGSKK11 pKa = 10.0 APKK14 pKa = 9.43 GARR17 pKa = 11.84 PVQTGNSANGVSGSNASMKK36 pKa = 10.38 KK37 pKa = 9.87 ILDD40 pKa = 3.99 ALPGVSPKK48 pKa = 10.06 FVRR51 pKa = 11.84 RR52 pKa = 11.84 VLRR55 pKa = 11.84 GRR57 pKa = 11.84 RR58 pKa = 11.84 LGITATVLAVYY69 pKa = 10.1 NAIQRR74 pKa = 11.84 ADD76 pKa = 3.92 PEE78 pKa = 4.71 DD79 pKa = 5.1 PICSSRR85 pKa = 11.84 SHH87 pKa = 5.17 TNKK90 pKa = 9.39 WLNAHH95 pKa = 6.57 LNGVASQRR103 pKa = 11.84 QAKK106 pKa = 8.3 TKK108 pKa = 10.46 GRR110 pKa = 11.84 TEE112 pKa = 3.91 PLIKK116 pKa = 10.16 QAADD120 pKa = 3.26 EE121 pKa = 4.46 LPNNKK126 pKa = 8.32 RR127 pKa = 11.84 TTGKK131 pKa = 10.6 AEE133 pKa = 4.15 PKK135 pKa = 9.99 QGNAKK140 pKa = 9.69 SRR142 pKa = 11.84 RR143 pKa = 11.84 AEE145 pKa = 3.92 RR146 pKa = 11.84 RR147 pKa = 11.84 AHH149 pKa = 5.84 LQPLVRR155 pKa = 11.84 GADD158 pKa = 3.3 AKK160 pKa = 10.83 LAAAKK165 pKa = 9.82 HH166 pKa = 5.65 RR167 pKa = 11.84 SAGGPAMGMSVAWQAEE183 pKa = 4.45 ANKK186 pKa = 10.6 APLPVTKK193 pKa = 10.24 RR194 pKa = 11.84 RR195 pKa = 11.84 PKK197 pKa = 9.87 EE198 pKa = 3.66 SRR200 pKa = 11.84 IKK202 pKa = 10.42 YY203 pKa = 9.73 LIRR206 pKa = 11.84 SYY208 pKa = 10.73 EE209 pKa = 3.88 AVIARR214 pKa = 11.84 GDD216 pKa = 3.61 KK217 pKa = 10.41 LHH219 pKa = 5.67 QQGKK223 pKa = 9.0 IVPEE227 pKa = 4.0 NLHH230 pKa = 5.98 LQADD234 pKa = 3.84 KK235 pKa = 11.48 LEE237 pKa = 4.21 NLLRR241 pKa = 11.84 EE242 pKa = 4.14 EE243 pKa = 4.18 RR244 pKa = 11.84 EE245 pKa = 4.01 KK246 pKa = 11.27 VRR248 pKa = 11.84 GHH250 pKa = 5.95 NKK252 pKa = 9.34 VNPVRR257 pKa = 11.84 LNAKK261 pKa = 9.35 KK262 pKa = 10.09 RR263 pKa = 11.84 RR264 pKa = 11.84 SGLRR268 pKa = 11.84 RR269 pKa = 11.84 QNPEE273 pKa = 3.74 LEE275 pKa = 4.16 AVRR278 pKa = 11.84 SKK280 pKa = 10.74 RR281 pKa = 11.84 AAAVPMNLNKK291 pKa = 10.52 LLGQTQGPLSSVLGNAGLAKK311 pKa = 9.45 TVEE314 pKa = 4.61 SILPEE319 pKa = 4.26 NGLTVTIKK327 pKa = 10.47 PSSQRR332 pKa = 11.84 EE333 pKa = 3.81 RR334 pKa = 11.84 KK335 pKa = 8.83 NARR338 pKa = 11.84 KK339 pKa = 9.17 AQVQRR344 pKa = 11.84 KK345 pKa = 7.87
Molecular weight: 37.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.207
IPC2_protein 10.014
IPC_protein 11.228
Toseland 11.506
ProMoST 11.828
Dawson 11.52
Bjellqvist 11.403
Wikipedia 11.901
Rodwell 11.623
Grimsley 11.55
Solomon 11.901
Lehninger 11.828
Nozaki 11.491
DTASelect 11.403
Thurlkill 11.491
EMBOSS 11.959
Sillero 11.491
Patrickios 11.33
IPC_peptide 11.915
IPC2_peptide 10.804
IPC2.peptide.svr19 9.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1722
345
527
430.5
47.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.266 ± 0.826
1.626 ± 0.373
4.413 ± 0.891
5.285 ± 0.704
2.381 ± 0.781
7.491 ± 0.871
2.439 ± 0.476
3.194 ± 0.442
5.11 ± 1.072
7.085 ± 1.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.207 ± 0.469
4.181 ± 0.485
6.214 ± 0.658
4.297 ± 0.605
9.001 ± 0.339
6.33 ± 0.493
5.691 ± 0.603
7.607 ± 0.605
1.568 ± 0.246
2.613 ± 0.436
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here