Staphylococcus phage P630

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Biseptimavirus; Staphylococcus virus P630

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9H0U8|A0A1X9H0U8_9CAUD Uncharacterized protein OS=Staphylococcus phage P630 OX=1735646 GN=P630_63 PE=4 SV=1
MM1 pKa = 6.89QQQAYY6 pKa = 9.38INATIDD12 pKa = 2.95IRR14 pKa = 11.84IPTEE18 pKa = 3.49VEE20 pKa = 3.58YY21 pKa = 10.91QYY23 pKa = 11.51FDD25 pKa = 4.86DD26 pKa = 3.89VDD28 pKa = 3.85KK29 pKa = 11.42EE30 pKa = 4.27KK31 pKa = 10.38EE32 pKa = 4.13TLADD36 pKa = 3.57YY37 pKa = 10.81LYY39 pKa = 10.9NNPDD43 pKa = 3.71EE44 pKa = 4.28ILEE47 pKa = 3.92YY48 pKa = 11.25DD49 pKa = 3.43NLKK52 pKa = 9.73IRR54 pKa = 11.84NVNIEE59 pKa = 3.84VEE61 pKa = 4.2

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9H083|A0A1X9H083_9CAUD Uncharacterized protein OS=Staphylococcus phage P630 OX=1735646 GN=P630_14 PE=4 SV=1
MM1 pKa = 7.2VALLKK6 pKa = 10.65SLEE9 pKa = 3.97RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84LMITISTMLQFGLFLIALIGLVIKK36 pKa = 10.76LIEE39 pKa = 4.3LSNKK43 pKa = 9.19KK44 pKa = 10.07

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12402

39

1509

193.8

22.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.644 ± 0.42

0.54 ± 0.088

6.402 ± 0.365

7.862 ± 0.483

4.217 ± 0.337

5.628 ± 0.614

1.661 ± 0.128

7.862 ± 0.277

9.547 ± 0.312

8.362 ± 0.355

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.435 ± 0.175

6.596 ± 0.233

2.556 ± 0.169

3.903 ± 0.188

4.33 ± 0.223

5.652 ± 0.271

5.475 ± 0.221

5.773 ± 0.207

1.145 ± 0.149

4.411 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski