Hydrogenophaga sp. IBVHS2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Hydrogenophaga; unclassified Hydrogenophaga

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2952 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2Q8V0|A0A1Y2Q8V0_9BURK Cytochrome C assembly protein OS=Hydrogenophaga sp. IBVHS2 OX=1985170 GN=CAP38_09975 PE=4 SV=1
MM1 pKa = 8.07PEE3 pKa = 4.33TPPDD7 pKa = 3.63SPRR10 pKa = 11.84DD11 pKa = 3.76DD12 pKa = 3.75SHH14 pKa = 7.48PPASLPCVLVFNANDD29 pKa = 3.67PSGAGGLSADD39 pKa = 3.97LTAMSSASVHH49 pKa = 5.32VLSVVTGAYY58 pKa = 10.11VRR60 pKa = 11.84DD61 pKa = 3.53TSEE64 pKa = 3.99VHH66 pKa = 6.36DD67 pKa = 5.37HH68 pKa = 6.6IAFDD72 pKa = 3.81EE73 pKa = 4.36EE74 pKa = 4.6AVVDD78 pKa = 4.0QARR81 pKa = 11.84CALEE85 pKa = 4.41DD86 pKa = 3.59MPVQAFKK93 pKa = 11.21VGFVGTPEE101 pKa = 4.1NLSAIAGITSDD112 pKa = 3.78YY113 pKa = 10.64TDD115 pKa = 3.86VPVVAYY121 pKa = 9.63MPDD124 pKa = 3.82LSWWDD129 pKa = 3.38EE130 pKa = 4.05MAIDD134 pKa = 4.36TYY136 pKa = 11.61LDD138 pKa = 3.49AFTEE142 pKa = 4.16LLLPQTTVLVGNHH155 pKa = 5.01STLCRR160 pKa = 11.84WLLPEE165 pKa = 4.31WEE167 pKa = 4.55GDD169 pKa = 3.41RR170 pKa = 11.84PPNARR175 pKa = 11.84EE176 pKa = 3.61IARR179 pKa = 11.84AAAVHH184 pKa = 5.85GVPYY188 pKa = 10.28TLVTGFNAADD198 pKa = 3.34QHH200 pKa = 6.63LEE202 pKa = 3.83SHH204 pKa = 6.67LASPEE209 pKa = 4.08SVLASARR216 pKa = 11.84YY217 pKa = 9.12EE218 pKa = 3.88RR219 pKa = 11.84FEE221 pKa = 4.15ATFSGAGDD229 pKa = 3.74TLSAALCALIGGGADD244 pKa = 3.5LQSACAEE251 pKa = 3.93ALTYY255 pKa = 10.72LDD257 pKa = 3.41QCLDD261 pKa = 3.39AGFQPGMGHH270 pKa = 7.25AVPDD274 pKa = 3.71RR275 pKa = 11.84LFWAHH280 pKa = 6.67EE281 pKa = 4.17DD282 pKa = 4.25EE283 pKa = 5.55PDD285 pKa = 4.97DD286 pKa = 5.56DD287 pKa = 5.66AADD290 pKa = 4.07PQQDD294 pKa = 3.38AGTDD298 pKa = 3.6STLDD302 pKa = 3.64ADD304 pKa = 5.59DD305 pKa = 5.23FPLDD309 pKa = 3.9TTRR312 pKa = 11.84HH313 pKa = 4.6

Molecular weight: 33.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2Q4S1|A0A1Y2Q4S1_9BURK ABC transporter OS=Hydrogenophaga sp. IBVHS2 OX=1985170 GN=CAP38_13960 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.41GRR39 pKa = 11.84KK40 pKa = 8.68RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2952

0

2952

976957

24

5839

330.9

35.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.856 ± 0.071

0.912 ± 0.015

5.396 ± 0.039

5.1 ± 0.04

3.32 ± 0.027

8.48 ± 0.046

2.368 ± 0.027

4.02 ± 0.031

2.72 ± 0.048

11.068 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.022

2.344 ± 0.027

5.653 ± 0.038

4.262 ± 0.025

7.482 ± 0.047

5.011 ± 0.032

5.195 ± 0.033

7.865 ± 0.036

1.572 ± 0.022

1.917 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski