Hydrogenophaga sp. IBVHS2
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2952 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y2Q8V0|A0A1Y2Q8V0_9BURK Cytochrome C assembly protein OS=Hydrogenophaga sp. IBVHS2 OX=1985170 GN=CAP38_09975 PE=4 SV=1
MM1 pKa = 8.07 PEE3 pKa = 4.33 TPPDD7 pKa = 3.63 SPRR10 pKa = 11.84 DD11 pKa = 3.76 DD12 pKa = 3.75 SHH14 pKa = 7.48 PPASLPCVLVFNANDD29 pKa = 3.67 PSGAGGLSADD39 pKa = 3.97 LTAMSSASVHH49 pKa = 5.32 VLSVVTGAYY58 pKa = 10.11 VRR60 pKa = 11.84 DD61 pKa = 3.53 TSEE64 pKa = 3.99 VHH66 pKa = 6.36 DD67 pKa = 5.37 HH68 pKa = 6.6 IAFDD72 pKa = 3.81 EE73 pKa = 4.36 EE74 pKa = 4.6 AVVDD78 pKa = 4.0 QARR81 pKa = 11.84 CALEE85 pKa = 4.41 DD86 pKa = 3.59 MPVQAFKK93 pKa = 11.21 VGFVGTPEE101 pKa = 4.1 NLSAIAGITSDD112 pKa = 3.78 YY113 pKa = 10.64 TDD115 pKa = 3.86 VPVVAYY121 pKa = 9.63 MPDD124 pKa = 3.82 LSWWDD129 pKa = 3.38 EE130 pKa = 4.05 MAIDD134 pKa = 4.36 TYY136 pKa = 11.61 LDD138 pKa = 3.49 AFTEE142 pKa = 4.16 LLLPQTTVLVGNHH155 pKa = 5.01 STLCRR160 pKa = 11.84 WLLPEE165 pKa = 4.31 WEE167 pKa = 4.55 GDD169 pKa = 3.41 RR170 pKa = 11.84 PPNARR175 pKa = 11.84 EE176 pKa = 3.61 IARR179 pKa = 11.84 AAAVHH184 pKa = 5.85 GVPYY188 pKa = 10.28 TLVTGFNAADD198 pKa = 3.34 QHH200 pKa = 6.63 LEE202 pKa = 3.83 SHH204 pKa = 6.67 LASPEE209 pKa = 4.08 SVLASARR216 pKa = 11.84 YY217 pKa = 9.12 EE218 pKa = 3.88 RR219 pKa = 11.84 FEE221 pKa = 4.15 ATFSGAGDD229 pKa = 3.74 TLSAALCALIGGGADD244 pKa = 3.5 LQSACAEE251 pKa = 3.93 ALTYY255 pKa = 10.72 LDD257 pKa = 3.41 QCLDD261 pKa = 3.39 AGFQPGMGHH270 pKa = 7.25 AVPDD274 pKa = 3.71 RR275 pKa = 11.84 LFWAHH280 pKa = 6.67 EE281 pKa = 4.17 DD282 pKa = 4.25 EE283 pKa = 5.55 PDD285 pKa = 4.97 DD286 pKa = 5.56 DD287 pKa = 5.66 AADD290 pKa = 4.07 PQQDD294 pKa = 3.38 AGTDD298 pKa = 3.6 STLDD302 pKa = 3.64 ADD304 pKa = 5.59 DD305 pKa = 5.23 FPLDD309 pKa = 3.9 TTRR312 pKa = 11.84 HH313 pKa = 4.6
Molecular weight: 33.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.923
IPC_protein 3.935
Toseland 3.719
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.872
Rodwell 3.77
Grimsley 3.63
Solomon 3.923
Lehninger 3.884
Nozaki 4.037
DTASelect 4.304
Thurlkill 3.77
EMBOSS 3.872
Sillero 4.062
Patrickios 1.926
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.942
Protein with the highest isoelectric point:
>tr|A0A1Y2Q4S1|A0A1Y2Q4S1_9BURK ABC transporter OS=Hydrogenophaga sp. IBVHS2 OX=1985170 GN=CAP38_13960 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.07 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.91 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.7 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.41 GRR39 pKa = 11.84 KK40 pKa = 8.68 RR41 pKa = 11.84 LSVV44 pKa = 3.2
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2952
0
2952
976957
24
5839
330.9
35.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.856 ± 0.071
0.912 ± 0.015
5.396 ± 0.039
5.1 ± 0.04
3.32 ± 0.027
8.48 ± 0.046
2.368 ± 0.027
4.02 ± 0.031
2.72 ± 0.048
11.068 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.459 ± 0.022
2.344 ± 0.027
5.653 ± 0.038
4.262 ± 0.025
7.482 ± 0.047
5.011 ± 0.032
5.195 ± 0.033
7.865 ± 0.036
1.572 ± 0.022
1.917 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here