Sewage-associated circular DNA virus-12

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075J453|A0A075J453_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-12 OX=1519388 PE=4 SV=1
MM1 pKa = 7.48ALKK4 pKa = 10.4ARR6 pKa = 11.84YY7 pKa = 8.15WCFTLNNWCDD17 pKa = 3.61GEE19 pKa = 4.26LLEE22 pKa = 4.82IAKK25 pKa = 10.56FSGRR29 pKa = 11.84YY30 pKa = 5.61LTYY33 pKa = 9.95GFEE36 pKa = 4.33IGEE39 pKa = 4.44SGTPHH44 pKa = 5.81LQGYY48 pKa = 8.58CEE50 pKa = 4.22YY51 pKa = 9.75EE52 pKa = 4.14NPVTMGRR59 pKa = 11.84VKK61 pKa = 10.71KK62 pKa = 10.46DD63 pKa = 2.84ISDD66 pKa = 3.64RR67 pKa = 11.84CHH69 pKa = 7.16VEE71 pKa = 3.87VRR73 pKa = 11.84KK74 pKa = 8.62GTQLQAVSYY83 pKa = 8.5CHH85 pKa = 7.42KK86 pKa = 10.6DD87 pKa = 3.93GNWLEE92 pKa = 4.22YY93 pKa = 10.83GEE95 pKa = 4.43KK96 pKa = 10.44AAGQGHH102 pKa = 6.96RR103 pKa = 11.84SDD105 pKa = 4.79LDD107 pKa = 3.58DD108 pKa = 3.53VARR111 pKa = 11.84DD112 pKa = 3.82VLDD115 pKa = 3.99KK116 pKa = 11.05KK117 pKa = 11.33SLMEE121 pKa = 4.24IANDD125 pKa = 4.56HH126 pKa = 5.37PTSWIRR132 pKa = 11.84YY133 pKa = 7.42SRR135 pKa = 11.84GIQSLANSQPPPRR148 pKa = 11.84WRR150 pKa = 11.84DD151 pKa = 3.48VLVLVYY157 pKa = 9.92WGVTGTGKK165 pKa = 7.99TRR167 pKa = 11.84RR168 pKa = 11.84AMLEE172 pKa = 3.49PSAYY176 pKa = 9.72KK177 pKa = 10.64LNQNTNGTLWFDD189 pKa = 4.03GYY191 pKa = 10.97DD192 pKa = 3.69GQSTLVIDD200 pKa = 5.94DD201 pKa = 4.29FYY203 pKa = 11.9GWIKK207 pKa = 10.77YY208 pKa = 10.42GEE210 pKa = 4.82LLTLLDD216 pKa = 4.97GYY218 pKa = 9.6PYY220 pKa = 10.28RR221 pKa = 11.84CQVKK225 pKa = 10.41GGMAWARR232 pKa = 11.84WDD234 pKa = 3.7TVIITSNKK242 pKa = 9.7CPDD245 pKa = 3.16DD246 pKa = 4.1WYY248 pKa = 10.54PLVPDD253 pKa = 4.16TAALKK258 pKa = 10.48RR259 pKa = 11.84RR260 pKa = 11.84ISAIRR265 pKa = 11.84YY266 pKa = 7.13FEE268 pKa = 5.37PILDD272 pKa = 3.77QKK274 pKa = 11.42

Molecular weight: 31.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075J453|A0A075J453_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-12 OX=1519388 PE=4 SV=1
MM1 pKa = 7.57FLRR4 pKa = 11.84PQMPRR9 pKa = 11.84GYY11 pKa = 10.61YY12 pKa = 9.68KK13 pKa = 8.91PTYY16 pKa = 9.84RR17 pKa = 11.84KK18 pKa = 7.74RR19 pKa = 11.84TYY21 pKa = 10.52RR22 pKa = 11.84KK23 pKa = 7.73RR24 pKa = 11.84TYY26 pKa = 7.41TRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84NTYY33 pKa = 9.58ARR35 pKa = 11.84KK36 pKa = 9.36KK37 pKa = 7.86WSPSIKK43 pKa = 10.25YY44 pKa = 10.05KK45 pKa = 10.98SEE47 pKa = 3.92TKK49 pKa = 9.78WYY51 pKa = 10.06DD52 pKa = 3.11GTAGGGGIGNMPAAWNRR69 pKa = 11.84YY70 pKa = 8.71DD71 pKa = 4.07LSCPITQGVTSVSRR85 pKa = 11.84VGNNIIAKK93 pKa = 9.82SLGISFDD100 pKa = 3.36VTRR103 pKa = 11.84NASSSTPQQRR113 pKa = 11.84MRR115 pKa = 11.84FMVIDD120 pKa = 3.72YY121 pKa = 8.08PRR123 pKa = 11.84SEE125 pKa = 4.18GSIPAAAEE133 pKa = 4.26FFQTTGVFLPQRR145 pKa = 11.84NLSWVKK151 pKa = 9.94EE152 pKa = 3.95FHH154 pKa = 6.23VLADD158 pKa = 3.62RR159 pKa = 11.84VITVDD164 pKa = 3.18SASRR168 pKa = 11.84NSQMGKK174 pKa = 10.24AFPII178 pKa = 4.07

Molecular weight: 20.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

452

178

274

226.0

25.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.195 ± 0.326

1.77 ± 0.72

5.973 ± 1.552

3.761 ± 0.902

3.097 ± 0.833

8.186 ± 0.526

1.327 ± 0.456

5.088 ± 0.316

5.973 ± 0.123

6.858 ± 2.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.559

3.982 ± 0.305

5.088 ± 0.651

3.761 ± 0.102

8.186 ± 1.483

6.858 ± 1.605

6.637 ± 0.732

5.531 ± 0.052

3.319 ± 0.639

5.973 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski