Streptococcus satellite phage Javan332

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKG1|A0A4D5ZKG1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan332 OX=2558642 GN=JavanS332_0011 PE=4 SV=1
MM1 pKa = 7.05VKK3 pKa = 10.23KK4 pKa = 10.44DD5 pKa = 3.18IYY7 pKa = 10.72DD8 pKa = 3.49WPEE11 pKa = 4.68GEE13 pKa = 5.69DD14 pKa = 3.86DD15 pKa = 5.88NLDD18 pKa = 3.76EE19 pKa = 4.85LLKK22 pKa = 10.69KK23 pKa = 10.6AYY25 pKa = 9.01EE26 pKa = 4.02RR27 pKa = 11.84NKK29 pKa = 10.56NKK31 pKa = 9.3TVEE34 pKa = 4.12EE35 pKa = 4.75LDD37 pKa = 4.05AEE39 pKa = 3.9WDD41 pKa = 3.79EE42 pKa = 4.49FVKK45 pKa = 10.72SLKK48 pKa = 10.89LEE50 pKa = 4.53TII52 pKa = 3.99

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZK04|A0A4D5ZK04_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan332 OX=2558642 GN=JavanS332_0015 PE=4 SV=1
MM1 pKa = 7.64FDD3 pKa = 4.35GFPEE7 pKa = 4.13RR8 pKa = 11.84PRR10 pKa = 11.84YY11 pKa = 8.12MKK13 pKa = 9.81RR14 pKa = 11.84DD15 pKa = 3.77RR16 pKa = 11.84YY17 pKa = 9.2HH18 pKa = 5.94KK19 pKa = 10.09HH20 pKa = 4.58YY21 pKa = 10.97KK22 pKa = 10.24KK23 pKa = 10.13FLKK26 pKa = 10.6YY27 pKa = 9.68IEE29 pKa = 4.58KK30 pKa = 10.6GDD32 pKa = 3.91RR33 pKa = 11.84FWLNGSRR40 pKa = 11.84LL41 pKa = 3.57

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

3324

39

498

138.5

16.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.077 ± 0.294

0.421 ± 0.103

5.686 ± 0.36

8.574 ± 0.666

4.302 ± 0.342

5.054 ± 0.37

1.805 ± 0.216

6.408 ± 0.49

10.078 ± 0.491

9.597 ± 0.431

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.798 ± 0.286

4.422 ± 0.311

3.008 ± 0.468

4.874 ± 0.308

5.235 ± 0.417

5.024 ± 0.274

6.408 ± 0.427

4.723 ± 0.445

0.903 ± 0.107

4.603 ± 0.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski