Laccaria bicolor (strain S238N-H82 / ATCC MYA-4686) (Bicoloured deceiver) (Laccaria laccata var. bicolor)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Tricholomataceae; Laccaria; Laccaria bicolor

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17895 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0DLC1|B0DLC1_LACBS Predicted protein OS=Laccaria bicolor (strain S238N-H82 / ATCC MYA-4686) OX=486041 GN=LACBIDRAFT_304307 PE=4 SV=1
MM1 pKa = 7.64CLFFSDD7 pKa = 4.19YY8 pKa = 10.46NVSVFSDD15 pKa = 3.9YY16 pKa = 11.13NVSAFFITNVIGDD29 pKa = 3.56MSIFILAICPVLSHH43 pKa = 6.53EE44 pKa = 4.8KK45 pKa = 10.38SLSYY49 pKa = 10.31LIKK52 pKa = 10.36GVQDD56 pKa = 3.8SVPALPDD63 pKa = 3.19NTDD66 pKa = 3.64DD67 pKa = 3.78VVTMISATEE76 pKa = 3.91PDD78 pKa = 3.88APVPPSNSLSSATAEE93 pKa = 4.05GMNDD97 pKa = 3.74EE98 pKa = 5.81DD99 pKa = 4.69EE100 pKa = 5.42DD101 pKa = 4.81DD102 pKa = 4.21PGRR105 pKa = 11.84PHH107 pKa = 7.75LPPCIEE113 pKa = 4.99LISLGHH119 pKa = 6.59FDD121 pKa = 4.29NIKK124 pKa = 9.85IAALNSVV131 pKa = 3.55

Molecular weight: 14.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0DS62|B0DS62_LACBS Predicted protein OS=Laccaria bicolor (strain S238N-H82 / ATCC MYA-4686) OX=486041 GN=LACBIDRAFT_316158 PE=4 SV=1
MM1 pKa = 7.33GRR3 pKa = 11.84GRR5 pKa = 11.84ALGLEE10 pKa = 4.21RR11 pKa = 11.84WRR13 pKa = 11.84LIGAGRR19 pKa = 11.84ILGRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84VLRR30 pKa = 11.84TPTANKK36 pKa = 7.64ITSAIPPTLLNHH48 pKa = 7.06AA49 pKa = 4.96

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17895

0

17895

6645138

42

4776

371.3

41.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.734 ± 0.018

1.491 ± 0.008

5.416 ± 0.015

5.674 ± 0.017

3.91 ± 0.013

6.211 ± 0.016

2.794 ± 0.009

5.019 ± 0.014

4.789 ± 0.019

9.396 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.082 ± 0.006

3.676 ± 0.011

6.592 ± 0.023

3.825 ± 0.013

6.047 ± 0.018

8.862 ± 0.023

6.212 ± 0.014

6.19 ± 0.014

1.488 ± 0.007

2.591 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski